We narrowed to 11,284 results for: Vars;

1. Designed VEGF-binding scFv G6des13

   Plasmid

   #110213

   Purpose

   Yeast Surface Display of G6des13

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   G6des13
   

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   L A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95F

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEMS2214

   Plasmid

   #158417

   Purpose

   AAV plasmid with Ple265 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple265 (PCP2 Human)
   

   Use

   AAV

   Promoter

   Ple265

   Available Since

   Feb. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIYN3

   Plasmid

   #197099

   Purpose

   To express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.

   Depositor

   Kensaku Sakamoto

   Article

   Mukai et al Biochem Biophys Res Commun. 2011 Aug 12;

   Insert

   TyrRS variant, Nap3 tRNA
   

   Expression

   Bacterial

   Mutation

   Ala70, Thr158, Ser159, Arg286 (TyrRS)

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Designed VEGF-binding scFv G6des1

   Plasmid

   #110212

   Purpose

   Yeast Surface Display of G6des1

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   G6des1
   

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   L A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-ASAP2s

   Plasmid

   #101276

   Purpose

   Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn (human synapsin)

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. NKP90 RFP (787)

   Plasmid

   #62001

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   NKP90 (TMEM126A Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   pgk

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pEMS2284

   Plasmid

   #158419

   Purpose

   AAV plasmid with Ple349 (PDE6H MiniPromoter) driving expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple349 (PDE6H Human)
   

   Use

   AAV

   Promoter

   Ple349

   Available Since

   Feb. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pαH-S-RRAR

   Plasmid

   #164569

   Purpose

   SARS-CoV-2 Spike protein with Furin site restored (S-RRAR variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-RRAR variant)
   

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3_GLP1R-V2R-NTEV-TCS-GV-2xHA

   Plasmid

   #194380

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)
   

   Tags

   2xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_HCLS1-1/1

    Plasmid

    #91437

    Purpose

    Protein expression and purification of human SH3 domain construct HCLS1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    HCLS1-1/1 (HCLS1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiU6-hASCL1 gRNA_1-MS2-Puro

    Plasmid

    #192670

    Purpose

    Lentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human ASCL1 activating gRNA #1 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEMS2021

    Plasmid

    #49128

    Purpose

    AAV plasmid with DCX (Ple302) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple302-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    DCX

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pJC5-4-insulator-BREreporter-insulator

    Plasmid

    #171499

    Purpose

    injections to make Bmp reporter mouse transgenic line

    Depositor

    James Briscoe

    Article

    Ivanovitch et al PLoS Biol. 2021 May 17;19(5):e3001200. d

    Insert

    Insulator BRE-MLP:H2B-Turqoise
    

    Expression

    Mammalian

    Promoter

    BRE-MLP

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

14. pET-KIX (586-672)

    Plasmid

    #99336

    Purpose

    Bacterial expression of mouse CBP KIX domain

    Depositor

    Peter Wright

    Article

    Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

    Insert

    CBP KIX (586-672) (Crebbp Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-PSAT

    Plasmid

    #74115

    Purpose

    Expression of PSAT

    Depositor

    Lewis Cantley

    Article

    Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

    Insert

    pET28a-PSAT (PSAT1 Human)
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. KIF5C(1-560)-2xmCitrine-GFP11

    Plasmid

    #61667

    Purpose

    Tandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF5C(1-560)-2xmCitrine-GFP11 (Kif5c Rat)
    

    Expression

    Mammalian

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-LYRa11-RBD-8h

    Plasmid

    #161822

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T324-D522)

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Talin1 head-mCherry

    Plasmid

    #212005

    Purpose

    Expression of mouse talin1 head (1-490)-mCherry in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Cell Sci. 2019 Apr 3;132(7):jcs226514.

    Insert

    Mouse talin1 head domain (1-490) (Tln1 Mouse)
    

    Tags

    mCherry fluorescent protein

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-RsSHC014-RBD-8h

    Plasmid

    #161826

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRC/CMV-JAK1K908E-VSV

    Plasmid

    #139357

    Purpose

    Expression of kinase-dead JAK1-K908E protein

    Depositor

    Sandra Pellegrini

    Article

    Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

    Insert

    JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
    

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    K908E

    Promoter

    CMV

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. PLX306 iCre tetO NICD rtTA

    Plasmid

    #158049

    Purpose

    lenti-viral construct with Cre recombinase and doxycycline inducible expression of mouse Notch intracellular domain

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    mouse Notch Intra Cellular Domain
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    tetO promoter

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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22. pLKO.1P POLE1_1

    Plasmid

    #160762

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3-SARS-1-RBD-8h

    Plasmid

    #161821

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T320-D518)

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. pEMS1997

    Plasmid

    #49127

    Purpose

    AAV plasmid with SLC6A4 (Ple198) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple198-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    SLC6A4

    Available Since

    May 7, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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25. pETM10 with rat FL Annexin A11

    Plasmid

    #164496

    Purpose

    For bacterial expression of rat His-tagged FL Annexin A11

    Depositor

    Anni Vedeler

    Article

    Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

    Insert

    rat FL Annexin A11 (Anxa11 Rat)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCR2.1_HBB_N

    Plasmid

    #87847

    Purpose

    pCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNA

    Depositor

    Marina Kleanthous

    Article

    Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

    Insert

    Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pPKm-248

    Plasmid

    #90504

    Purpose

    Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tPCYA - IRES - MTS - tHO1 - P2A - MTS - tFD - P2A - MTS - tFNR

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
    

    Use

    Lentiviral

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. Equalizer-L (mScarlet-I) plasmid with onboard mCitrine cassette

    Plasmid

    #169737

    Purpose

    Equalizer-L plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-tetR-P2A-mScarletI-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pGAPDH::H2B-mScarlet

    Plasmid

    #186764

    Purpose

    A plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    H2B-mScarlet
    

    Use

    Overexpression

    Tags

    H2B

    Promoter

    GAPDH

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pLV-hIP-dCas9-BSD

    Plasmid

    #183231

    Purpose

    Lentiviral vectors with the human insulin promoter driving expression of dCas9, in addition to a U6-driven sgRNA

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    Human insulin promoter

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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