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We narrowed to 14,052 results for: CRISPR-Cas9

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Showing: 3301 - 3320 of 14052 results

  12. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid
    #188899
    Purpose
    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Zim3-dCas9
    Use
    Lentiviral
    Tags
    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion
    Promoter
    SFFV
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188899-plasmid-map-sequence-id-374831

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  14. AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

    Plasmid
    #210710
    Purpose
    This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9
    Use
    AAV and CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A/N580A
    Promoter
    M11
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210710-plasmid-map-sequence-id-416264

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  17. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid
    #217800
    Purpose
    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217800-plasmid-map-sequence-id-432875

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  19. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118162
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118162-plasmid-map-sequence-id-240427

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  20. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118163
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118163-plasmid-map-sequence-id-240400

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Showing: 3301 - 3320 of 14052 results