We narrowed to 4,337 results for: U6 gRNA

1. BPK3082

   Plasmid

   #78742

   Purpose

   Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   LbCpf1 crRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Intron-Tagging-pX330-Cas9-Blast

   Plasmid

   #159741

   Purpose

   Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   donor plasmid targeting sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPK3079

   Plasmid

   #78741

   Purpose

   Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   AsCpf1 crRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLentiCRISPR_zeo_sgNT135

   Plasmid

   #183181

   Purpose

   pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   NT135
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. LbCas12a-crRNA

   Plasmid

   #213753

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-crRNA in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   LbCas12a-crRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJY-RpABE

   Plasmid

   #112872

   Purpose

   binary vector for ABE expression in Arabidopsis

   Depositor

   Jae-Young Yun, Jin-Soo Kim

   Article

   Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

   Inserts

   RPS5A promoter
   ABE7.10
   U6-sgRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLL5.0 shFmnl2-EGFP

   Plasmid

   #187255

   Purpose

   Lentiviral expression of shRNA targeting Fmnl2

   Depositor

   Alpha Yap

   Article

   Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

   Insert

   ShRNA for Fmnl2 (Fmnl2 Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LentiCRISPR-V2_hMSH2

   Plasmid

   #186155

   Purpose

   sgRNA targeting human MSH2 gene

   Depositor

   David McFadden

   Article

   Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

   Insert

   MSH2 (MSH2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PB-SAM-Dest

   Plasmid

   #102563

   Purpose

   Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   N/A

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. sgLenti

    Plasmid

    #105996

    Purpose

    single sgRNA expression vector

    Depositor

    Mike McManus

    Article

    Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; S.pyogenes sgrna expressio…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO-shSmad4

    Plasmid

    #37046

    Depositor

    Sam Thiagalingam

    Article

    Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

    Insert

    Smad4 (SMAD4 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. LentiCRISPRv2-KLF7-1

    Plasmid

    #169797

    Purpose

    Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    guide RNA for KLF7 neighbouring region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRB17

    Plasmid

    #52527

    Purpose

    serves as template for PCR-mediated fusion of U6-promoter with sgRNA

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Drosophila U6C promoter
    

    Use

    CRISPR; Blunt cloning kit

    Tags

    T7-promoter

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Intron-Tagging-pX330-Cas9-mCherry

    Plasmid

    #159742

    Purpose

    Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

    Insert

    donor plasmid targeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSicoR-ADAM29i1

    Plasmid

    #59606

    Purpose

    Expression of shRNA against human ADAM29

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    ADAM29 (ADAM29 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

16. SL2-sgTelo-MTSa/BFP/pdCas9-C1

    Plasmid

    #162760

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting telomeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgTelo-MTSa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAT15415-BEAR-GFP-target-mCherry

    Plasmid

    #162995

    Purpose

    plasmid expressing an sgRNA targeting the BEAR-GFP plasmid along with an mCherry marker

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    sgRNA targeting the BEAR-GFP plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLL5.0 shCAV1-NLS-mCherry

    Plasmid

    #187257

    Purpose

    Lentiviral expression of shRNA targeting Caveolin1 and NLS-mCherry

    Depositor

    Alpha Yap

    Article

    Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

    Insert

    ShRNA for Caveolin-1 (Cav1 Mouse)
    

    Use

    Lentiviral

    Tags

    NLS-mCherry

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SL2-sgTelo-MTSa/EGFP/pdCas9-C1

    Plasmid

    #162759

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting telomeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgTelo-MTSa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SL2-sgSat-MTSb/BFP/pdCas9-C1

    Plasmid

    #162761

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting centromeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgSat-MTSb)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge