19,775 results

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pHAGE-BRAF-V600E

Plasmid

#116204

Purpose

Lentiviral expression of BRAF V600E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600E

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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psfaRb-Tn5

Plasmid

#183638

Purpose

Expresses anti-rabbit nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Article

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti rabbit secondary nanobody fused to Tn5

Expression

Bacterial

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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PIK3CA-WT

Plasmid

#16643

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA (PIK3CA Human)

Tags

Flag

Expression

Mammalian

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc

Plasmid

#117728

Purpose

Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

NanoLuc

Use

Luciferase

Expression

Bacterial

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_r5-HT1.0

Plasmid

#208719

Purpose

Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Red fluorescent 5-HT sensor GRAB_r5-HT1.0

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTRANS_221

Plasmid

#91115

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pICH47761

Plasmid

#48003

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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DsRed2-Mito-7

Plasmid

#55838

Purpose

Localization: Mitochondria, Excitation: 558, Emission: 583

Depositor

Michael Davidson

Insert

Mito (COX8A Human)

Tags

DsRed2

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C1 EGFP-3XNLS

Plasmid

#58468

Purpose

Expresses a nuclear-targeted eGFP on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Insert

3X NLS with 3X stop codons

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-pBAD

Plasmid

#54844

Purpose

Localization: Protein Expression Vector , Excitation: 434, Emission: 474

Depositor

Michael Davidson

Type

Empty backbone

Tags

6x His, T7 tag, and TEV site

Expression

Bacterial

Promoter

araBAD

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

Plasmid

#68375

Purpose

Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and TALEN; Zfn

Tags

3xFLAG_Twin_Strep

Expression

Mammalian

Promoter

human PGK1

Available Since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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CLYBL-Bxb1-LP-v2-TC

Plasmid

#194327

Purpose

CLYBL targeting construct with the Bxb1 landing pad version 2 cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

Insert

loxP-EBFP2-attP-BleoR-lox251

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pALOD4

Plasmid

#111026

Purpose

Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A

Depositor

Arun Radhakrishnan

Article

Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

Insert

Domain 4 of Anthrolysin O

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: S404C, C472A

Promoter

T7

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-hALIX

Plasmid

#21504

Depositor

James Hurley

Article

Lee et al Science. 2008 Oct 24. 322(5901):576-80.

Insert

ALIX (PDCD6IP Human)

Tags

Cherry

Expression

Bacterial

Mutation

none

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only

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anxA2-GFP

Plasmid

#107196

Purpose

Expresses human annexin A2-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

annexin A2 (ANXA2 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Ala residue 65 in human sequence replaced by Glu …

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMPRAdonor2

Plasmid

#49353

Purpose

minP-luc2 donor plasmid for MPRA library construction

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

luc2

Use

Luciferase

Promoter

minP

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pHR_PGK

Plasmid

#79120

Purpose

Lentiviral vector for constitutive expression of genes (PGK promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-3xFLAG-V5-ccdB

Plasmid

#87072

Purpose

Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-rab7 WT

Plasmid

#12605

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

GFP and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pCAG-ChrimsonR-tdT

Plasmid

#59169

Purpose

Expression vector for ChrimsonR-tdTomato

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-tdTomato

Tags

tdTomato

Expression

Mammalian

Mutation

K176R

Promoter

CAG

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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