We narrowed to 9,393 results for: Coli

1. His SUMO TEV W22R SPOP 1-374

   Plasmid

   #197016

   Purpose

   E. coli expression vector

   Depositor

   Tanja Mittag

   Article

   Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

   Insert

   SPOP (Spop Mouse)
   

   Use

   Tags

   6His-SUMO-TEV

   Expression

   Bacterial

   Mutation

   W22R

   Promoter

   Available since

   April 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNIC-Bio2_MVPI_SMAC(delta(55))_9His

   Plasmid

   #197978

   Purpose

   E. coli expression of MVPI-SMAC(delta(55))_9His

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   Smac (DIABLO Human)
   

   Use

   Tags

   9His

   Expression

   Bacterial

   Mutation

   delta (1-55), A56M

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIC-Bio2_SMAC(delta(55))_Cys_9His

   Plasmid

   #197977

   Purpose

   E. coli expression of SMAC(delta(55))_Cys-9His

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   Smac (DIABLO Human)
   

   Use

   Tags

   9His

   Expression

   Bacterial

   Mutation

   delta (1-55), ins240C

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXD70Tet(DadRmt1)-DadR(ΔDBD)

   Plasmid

   #191637

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔDBD) complementation

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   DadR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ΔDBD

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXD70Tet(DadRmt2)-DadR(ΔTM)

   Plasmid

   #191638

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔTM) complementation

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   DadR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ΔTM

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXD70Tet(DadRmt4)-DadR(linker)

   Plasmid

   #191639

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(linker region shuffled) complementation

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   DadR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Linker region sequence shuffled

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXD70Tet(DadR-NFLAG)-N-3xFLAG-DadR

   Plasmid

   #191640

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR complementation

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   DadR
   

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. mRuby3 only VLP

   Plasmid

   #182429

   Purpose

   YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and mRuby3 tagged with VP2C (GAL10 promoter). mRuby3 was codon-optimised for E. coli. Contains uracil marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-mRuby3
   deltaVP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. QUAS-0-T7-TetO-GFP_ColE1

   Plasmid

   #171662

   Purpose

   QUAS directly upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli. Contains the ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-T7-TetO-GFP ssrA-T7 Stop
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET_His6-MBP-TEV-Cas6-RT-Cas1_WT

    Plasmid

    #186274

    Purpose

    Expresses Thiomicrospira Cas6-RT-Cas1 in E. coli

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicropira Cas6-RT-Cas1
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28b-bhaA

    Plasmid

    #167810

    Purpose

    Expresses His6-tagged BhaA in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

    Insert

    BhaA
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28b-bhaB1

    Plasmid

    #167811

    Purpose

    Expresses His6-tagged BhaB1 in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

    Insert

    BhaB1
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b-bhaB7

    Plasmid

    #167813

    Purpose

    Expresses His6-tagged BhaB7 in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

    Insert

    BhaB7
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET15b-bhaC1

    Plasmid

    #167814

    Purpose

    Expresses BhaC1 in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

    Insert

    BhaC1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSF-1b-S7-S83C/Δ157-178

    Plasmid

    #128592

    Purpose

    Expresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    S7 E.coli ribosomal protein (rpsG E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S83C, Δ157-178

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHBT-Mb(GDx-Clo_L10)

    Plasmid

    #183406

    Purpose

    Expresses Monobody GDx-Clo_L10 in E. coli

    Depositor

    Shohei Koide

    Article

    Kermani et al Nat Commun. 2020 Nov 27;11(1):6064. doi:

    Insert

    His tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)
    

    Use

    Tags

    His tag-Avi tag-TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PdnaK-IR2-IR2 GFP pZa

    Plasmid

    #170083

    Purpose

    GFP expression under the control of E. coli dnaK promoter engineered with double IR2 HAIR motif from M. tuberculosis

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PdnaK-IR2-IR2

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PdnaK-IR3 GFP pZa

    Plasmid

    #170084

    Purpose

    GFP expression under the control of E. coli dnaK promoter engineered with IR3 HAIR motif from M. tuberculosis

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PdnaK-IR3

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PdnaK-IR2 GFP pZa

    Plasmid

    #170081

    Purpose

    GFP expression under the control of E. coli dnaK promoter engineered with IR2 HAIR motif from M. tuberculosis

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PdnaK-IR2

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a(+)-HisTim12_CtoA

    Plasmid

    #170281

    Purpose

    Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

    Insert

    yeast Tim12 (TIM12 Budding Yeast)
    

    Use

    Tags

    TEV-cleavable N-terminal His tag (on backbone)

    Expression

    Bacterial

    Mutation

    mutated two non-conserved Cys to Ala

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET19b-rDAD2 cPGFP(N180)

    Plasmid

    #166001

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rDAD2 cPGFP(N180)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LSSmOrange- V49A (please see depositor comments)

    Promoter

    T7 promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r

    Plasmid

    #162965

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expression

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Halotag fusion tag with upstream E. coli ribosome binding site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r

    Plasmid

    #162964

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPB-tetpA(R1R4)-iRPT

    Plasmid

    #154882

    Purpose

    Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

    Depositor

    Hideyuki Okano

    Article

    Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

    Inserts

    iRFP713
    Puromycin resistance gene
    rtTAM2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-Avi-Coh (E154C)-HIS

    Plasmid

    #153447

    Purpose

    E. coli expression of Rc. CohE construct with E154C mutation.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.CohE
    

    Use

    Tags

    6xHis and Avi-tag

    Expression

    Bacterial

    Mutation

    E154C mutation on CohE

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRSFDuet1-tglA(C51)HIB

    Plasmid

    #134675

    Purpose

    Expresses His6-TglA(C51), TglH, TglI and TglB in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Inserts

    TglA(C51)
    TglH
    TglI
    TglB
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    insertion of cysteine 51

    Promoter

    T7

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pACYCDuet1-ammA(∆50-56)

    Plasmid

    #134684

    Purpose

    Expresses His6-AmmA(∆50-56) in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Insert

    ammA(del50-56)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    deletion of amino acids 50-56

    Promoter

    T7

    Available since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHis8-4b-McDAH

    Plasmid

    #140129

    Purpose

    Expresses McDAH with N-terminal 8xHis-tag in BL21 E. coli

    Depositor

    Jing-Ke Weng

    Article

    Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

    Insert

    Dechloroacutumine halogenase from Menispermum canadense
    

    Use

    Tags

    8x-His, TEV protease site (ENLYFQ)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSB4C5_J23101-vdh

    Plasmid

    #128130

    Purpose

    Expressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    vdh
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEX-4T1 mouse Tankyrase1 ARC2-3 (308-655)

    Plasmid

    #132637

    Purpose

    expresses mouse Tankyrase1 ARC2-3 (308-655) in E. coli

    Depositor

    Wenqing Xu

    Article

    Morrone et al Proc Natl Acad Sci U S A. 2012 Jan 31;10

    Insert

    Tankyrase1 (308-655)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBEAST_J23101-HipO

    Plasmid

    #128133

    Purpose

    The cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    HipO
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBEAST_J23101-vdh

    Plasmid

    #128134

    Purpose

    The cell-free adapted backbone, pBEAST, expressing vdh (the enzyme converting benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    vdh
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMH-HT_SsCHAD-H29A-R32A-R36A

    Plasmid

    #127786

    Purpose

    Expresses a SsCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    mutations in His29, Arg32 and Arg36 to Ala

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMH-HT_CtCHAD-R438A-K441A

    Plasmid

    #127791

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in Arg4…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTEI177

    Plasmid

    #126077

    Purpose

    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP1-10 and expression in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    ccdB Cassette (BsaI)
    GFP1-10
    

    Use

    Binary, module 1

    Tags

    linker (GTGGGGS)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTEI129

    Plasmid

    #126210

    Purpose

    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    ccdB Cassette (BsaI)
    GFP11x7 (5 aa linker)
    

    Use

    Binary, module 1

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTEI161

    Plasmid

    #126211

    Purpose

    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11 and expression in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    ccdB Cassette (BsaI)
    GFP11
    

    Use

    Binary, module 1

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. BPK2013-BsCas12b

    Plasmid

    #121952

    Purpose

    E. coli expression, protein purification

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    BsCas12b
    

    Use

    Tags

    10xHis tag and 3xHA tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET15b+R2(L171C)

    Plasmid

    #108239

    Purpose

    Expresses PdR-PCNA2(L171C) fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA2(L171C) fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    PCNA2,L171C

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CTL2 (M2225R)

    Plasmid

    #96952

    Purpose

    Expresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coli

    Depositor

    Douglas Rees

    Article

    Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

    Insert

    CTL2 (M222R) of PIEZO1
    

    Use

    Tags

    6x Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge