We narrowed to 7,181 results for: RAP

1. pRoCascade_gfp

   Plasmid

   #178771

   Purpose

   Expression of Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis

   Depositor

   Chase Beisel

   Article

   Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

   Insert

   Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRoDonor

   Plasmid

   #178773

   Purpose

   Expression of RoCAST left end, cam, RoCAST right end from Rippkaea orientalis

   Depositor

   Chase Beisel

   Article

   Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

   Insert

   RoCAST left end, cam, RoCAST right end from Rippkaea orientalis
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK6_016

   Plasmid

   #124058

   Purpose

   Encodes the extended Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   WPRE (extended)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJBL718

   Plasmid

   #140388

   Purpose

   Expresses MobR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mobO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-mobO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCVL SFFV Sbf-Sal linker

   Plasmid

   #50434

   Purpose

   Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).

   Depositor

   Andrew Scharenberg

   Article

   Boissel et al Nucleic Acids Res. 2013 Nov 26.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   May 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK2_024

   Plasmid

   #123716

   Purpose

   Encodes crippled mouse pPGK1 as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pPGK1c
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Crippled Kozac sequence

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pES002 (pTet-qacR wt)

   Plasmid

   #69031

   Purpose

   wild-type qacR downstream of a Tet promoter

   Depositor

   Richard Murray

   Article

   de Los Santos et al ACS Synth Biol. 2015 Aug 19.

   Insert

   qacR (qacR Synthetic)
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. cp-DdCBE G1333-C

   Plasmid

   #174557

   Purpose

   Chloroplast-targeting DdCBE (split DddA, G1333-C) Golden Gate destination vector

   Depositor

   Jin-Soo Kim

   Article

   Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

   Insert

   DdCBE G1333-C
   

   Use

   Tags

   FLAG

   Expression

   Plant

   Mutation

   Promoter

   PcUbi

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. px330 EQR

   Plasmid

   #101733

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRUSH

    Plasmid

    #54479

    Purpose

    Mouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to drive short RNAs, pgkneo, cassette flanked by loxP sites

    Depositor

    Laurie Jackson-Grusby

    Article

    Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1

    Type

    Empty backbone

    Use

    Cre/Lox, Mouse Targeting, and RNAi

    Tags

    SA-EGFP-polyA, U6 promoter, loxP, and pgk-neo

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

11. p3E-Dam-myc no-pA

    Plasmid

    #82600

    Purpose

    3' entry vector containing myc epitope tag fused to C-terminus of E. coli DNA adenine methyltransferase without pA for DamID

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    Dam-Myc (no pA)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pH6HTC_IGF2BP2b-Halo

    Plasmid

    #175318

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    IGF2BP2 (IGF2BP2 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Isoform B

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQCi

    Plasmid

    #154345

    Purpose

    Self-Cutting and Integrating CRISPR backbone (px458 derivative with Cas9-intron, sgRNA, and bait integration sites)

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pH6HTC_CCDC86-Halo

    Plasmid

    #175323

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    CCDC86 (CCDC86 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Tag 3A/myc-M1L195V

    Plasmid

    #87716

    Purpose

    Expresses myc-M1L195V spastin isoform in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    M1 isoform; L195V

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pADH118-2

    Plasmid

    #90984

    Purpose

    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. albicans LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX-V5-TRAF2 S11A-puro

    Plasmid

    #44112

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 S11A (TRAF2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Serine 11 to Alanine

    Promoter

    Available since

    July 22, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pOVC1_ScPH1-S_X

    Plasmid

    #131184

    Purpose

    Expresses ScPH1-S (Synechosystis PCC6803) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    ScPH1-S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK3a_001

    Plasmid

    #123754

    Purpose

    Encodes plasma membrane localization signal from Lyn kinase as a Type 3a part to be used in the MTK

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    LYN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. TOPO CMV

    Plasmid

    #68454

    Purpose

    TOPO construct expressing CMV for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Cytomegalovirus Immediate-Early Promoter
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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