174,557 results

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pET30a-AlkB-D135S

Plasmid

#79051

Purpose

Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

Depositor

Tao Pan

Article

Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

Insert

AlkB (alkB E.coli)

Tags

His tag

Expression

Bacterial

Mutation

A truncated AlkB with deletion of the amino (N)-t…

Promoter

T7

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD/His-miRFP670

Plasmid

#79984

Purpose

Monomeric near-infrared fluorescent protein miRFP670 in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP670

Expression

Bacterial

Promoter

araB

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pY002 (pFnCpf1_min)

Plasmid

#69975

Purpose

Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus without Cas genes nd artificial promoter

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-p150Glued

Plasmid

#36154

Depositor

David Stephens

Article

Watson et al J Cell Sci. 2006 Jul 1;119(Pt 13):2758-6

Insert

p150Glued (DCTN1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

see documentation

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHRIG-Akt1

Plasmid

#53583

Purpose

lentiviral expression of constitutively active human Akt1 and EGFP

Depositor

Heng Zhao

Article

Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

Insert

cAkt1 (AKT1 Human)

Use

Lentiviral

Tags

HA and IRES-EGFP

Expression

Mammalian

Mutation

constitutively active (myristoylated version)

Promoter

CMV

Available Since

Oct. 28, 2014

Availability

Academic Institutions and Nonprofits only

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BFP-Rab5

Plasmid

#49147

Purpose

mammalian expression of TagBFP-Rab5 fusion

Depositor

Gia Voeltz

Article

Friedman et al Mol Biol Cell. 2013 Apr;24(7):1030-40. d

Insert

Rab5 (RAB5B Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2+hSpCas9

Plasmid

#51815

Purpose

Encode hSpCas9 for in vitro transcription with Sp6

Depositor

Masato Kinoshita

Article

Ansai et al Biol Open. 2014 Apr 11;3(5):362-71. doi:

Insert

humanized S.pyogenes Cas9

Use

CRISPR; Medaka, zebrafish

Tags

3xFLAG

Expression

Mammalian

Promoter

SP6

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-hCAS-pA

Plasmid

#48625

Purpose

Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii, Kunihiko Naito

Article

Fujii et al Nucleic Acids Res. 2013 Aug 30.

Insert

Codon optimized Cas9

Use

CRISPR

Tags

FLAG, NLS, and polyA

Expression

Mammalian

Promoter

CAG promoter

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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5xGAL4-TATA-luciferase

Plasmid

#46756

Purpose

Luciferase reporter gene for factors with GAL4 DNA binding domains

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

Luciferase

Use

Luciferase

Promoter

minimal TATA

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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tetO-MEF2C

Plasmid

#46031

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

MEF2C (MEF2C Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Promoter

tetO

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-HA-hM3D(Gq)

Plasmid

#45547

Purpose

Expresses hM3D (Gq) neuronal activator

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

hM3D (Gq)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

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pBB542

Plasmid

#27395

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

dnaK, dnaJ, groESL

Expression

Bacterial

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-puro GFP-DFCP1

Plasmid

#38269

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

Zinc finger FYVE domain-containing protein 1 (Zfyve1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pYSEAP

Plasmid

#37326

Depositor

John Schiller

Article

Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

Insert

SEAP

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pT2/HB

Plasmid

#26557

Depositor

Perry Hackett

Insert

pT2/HB

Expression

Bacterial

Available Since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pBMN YFP-LID

Plasmid

#31767

Depositor

Thomas Wandless

Article

Bonger et al Nat Chem Biol. 2011 Jul 3. doi: 10.1038/

Insert

YFP-LID (FKBP1A Human)

Use

Retroviral

Tags

HA and YFP

Mutation

F36V on FKBP12 and additional 19 aa (TRGVEEVAEGVV…

Promoter

LTR

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

Plasmid

#31476

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d14 GFP donor EF1s I-SceI

Use

Lentiviral

Tags

HA and NLS

Mutation

GFP has 14 amino acids deleted from c terminus

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR5'_ubi:loxP-EGFP-loxP

Plasmid

#27322

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

ubiquitin:loxP-EGFP-loxP

Use

Multisite gateway 5' entry vector

Available Since

April 5, 2011

Availability

Academic Institutions and Nonprofits only

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pECFP-DEVDR-Venus

Plasmid

#24537

Depositor

Peter Sorger

Article

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

ECFP-DEVDR-Venus

Tags

ECFP and Venus

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
pSYFP2-C1

Plasmid

#22878

Depositor

Dorus Gadella

Article

Kremers et al Biochemistry. 2006 May 30. 45(21):6570-8

Insert

SYFP2

Expression

Mammalian

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

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