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pEF5/FRT/DEST-3xHA/mGli2 P5-6A
Plasmid#51253PurposeEncodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to AlaDepositorInsertGli2 (Gli2 Mouse)
UseFlpin systemTags3xHA-TEVExpressionMammalianMutationamino acids 923,956 mutated to AlaPromoterEF1aAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
NC1 pGLUE WTX (1-804)
Plasmid#36956DepositorInsertWTX (AMER1 Human)
UseTagsCBP tag, HA, and streptagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
CMV-10-3xFLAG-LGP2-MIII
Plasmid#58683PurposeExpresses LGP2 with a mutation to motif III in mammalian cellsDepositorInsertLGP2-MIII (DHX58 Human)
UseTags3x FLAGExpressionMammalianMutationthreonine and serine of helicase motif III mutate…PromoterCMVAvailable sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pET28A-p53 (1-73) (P27A)
Plasmid#62068PurposeExpression of human p53 (1-73) (P27A) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains TAD residues 1-73; P27APromoterT7Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
mChF-AIP (TD)
Plasmid#61529PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TD mutation)DepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TD mutation (see comments) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
mChF-AIP (TE)
Plasmid#61530PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TE mutation)DepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TE mutation (see comments) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-FLAG-SLC38A9.1 H60A
Plasmid#71863Purposelentiviral stable expressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon optimized, Histidine 60 mutated to AlaninePromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-FLAG-SLC38A9.1 L74A
Plasmid#71866Purposelentiviral stable expressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon optimized, Leucine 74 mutated to AlaninePromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-FLAG-SLC38A9.1 P90A
Plasmid#71867Purposelentiviral stable expressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon optimized, Proline 90 mutated to AlaninePromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-HA-MEK1 CBM
Plasmid#53163PurposeExpresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vectorDepositorInsertMEK1 (MAP2K1 Human)
UseTagsGST and HAExpressionBacterialMutationM187A/H188A/M230A/H239APromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-HA-MEK5 CBM-E
Plasmid#53168PurposeExpresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vectorDepositorInsertMEK5 (MAP2K5 Human)
UseTagsGST and HAExpressionBacterialMutationL280A/H281A/M323A/Q332APromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiX2-PURO-shDIXDC1-Ms-57
Plasmid#61232PurposeLentivirus expressing shRNA to mouse DIXDC1DepositorInsertDixdc1 (Dixdc1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP G protein Gamma 3(K68A,K69A,F70A,F71A) in pcDNA3.1
Plasmid#42189DepositorInsertGamma 3(K68A,K69A,F70A,F71A) (Gng3 Mouse)
UseTagsEGFPExpressionMammalianMutationK68A,K69A,F70A,F71APromoterCMVAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP G protein Gamma 3(A73L,L74I,L75S) in pcDNA3.1
Plasmid#42190DepositorInsertG protein Gamma 3(A73L,L74I,L75S) (Gng3 Mouse)
UseTagsEGFPExpressionMammalianMutationA73L,L74I,L75SPromoterCMVAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1puro-shWillin-B
Plasmid#40886DepositorInsertshWillin-B (FRMD6 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1puro-shWillin-A
Plasmid#40885DepositorInsertshWillin-A (FRMD6 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 FLAG FBXO31
Plasmid#236429Purposetransient overexpression of FBXO31 in mammalian cellsDepositorInsertFBXO31 (FBXO31 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
SB-CAG-mCherry-P2A-EIF3D-IP
Plasmid#236769PurposeA sleeping beauty-based vector containing CAG promoter-driven mCherry-P2A-EIF3D-IRES-Pac.DepositorInsertEIF3D (EIF3D Human)
UseSleeping beautyTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiSIV hSyn HA-NLS-SpCas9-NLS WPRE
Plasmid#236242PurposeLentiviral expression of SpCas9DepositorInsertSpCas9
UseLentiviralTagsExpressionMutationPromoterhuman Synapsin 1Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
ER-RspA-NFAST
Plasmid#233595PurposeExpression of RspA-NFAST on the ER membraneDepositorInsertRspA-NFAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
ER-long-RspA-NFAST
Plasmid#233600PurposeExpression of RspA-NFAST on the ER membrane after a 55 aminoacid linkerDepositorInsertRspA-NFAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
OMM-short-RspA-NFAST
Plasmid#233603PurposeExpression of RspA-NFAST on the outer mitochondrial membraneDepositorInsertRspA-NFAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
MKC177_CCR5(PGK-synEPOR)
Plasmid#232415PurposeAAV production plasmid for PGK(synEPOR) vector from Figs. 3-5 that mediates HDR at CCR5 locus using CCR5 gRNA. synEPOR is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.DepositorInsertTruncated Erythropoietin Receptor (EPOR Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
MKC176_EPOR(2A-synEPOR)
Plasmid#232414PurposeAAV production plasmid for EPOR(synEPOR) vector from Figs. 3-5 that mediates HDR at EPOR locus using EPOR gRNA. synEPOR is codon diverged to prevent premature recombination with EPOR locus.DepositorInsertTruncated Erythropoietin Receptor (EPOR Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0176 CMVTO-mono-hIL10-R5A11M-mouse_rens-3GS-IL10Ra
Plasmid#231202PurposeCageIL-10-mono (mouse renin cut-site)DepositorInsertmono-hIL10-R5A11M-mouse_rens-3GS-IL10Ra (IL10 Human)
UseTagsExpressionMammalianMutationN36I, N110I, K117N, F129LPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0266 CMVTO-IVT-UTR-mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin (FLP-IN)
Plasmid#231209PurposeSingle transcript mRNA cageIL-2 (mRens) + ER-renin (H2M)DepositorInsertUTR-mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin (IL2 Human)
UseTagsExpressionMammalianMutationL80F, R81D, L85V, I86V, I92FPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only
