We narrowed to 739 results for: Numb

1. 5mARF17 pGreenII0229

   Plasmid

   #12071

   Depositor

   David Bartel

   Article

   Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

   Insert

   5mARF17 (ARF17 Mustard Weed)
   

   Use

   Plant transformation via agrobacterium

   Tags

   Expression

   Plant

   Mutation

   miR160-resistant version of ARF17 has five silent…

   Promoter

   Available since

   June 2, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pEMS1925

   Plasmid

   #105869

   Purpose

   High copy number plasmid containing iCreERT2 synthesized fragment for constructing the HPRT plasmid

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   iCreERT2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMS1958

   Plasmid

   #82569

   Purpose

   High copy number plasmid containing PR2/3:Con MiniPromoter insert.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   PRS2/3:Con
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. 5mARF17 pGreenII0129

   Plasmid

   #12081

   Depositor

   David Bartel

   Article

   Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

   Insert

   5mARF17 (ARF17 Mustard Weed)
   

   Use

   Plant transformation via agrobacterium

   Tags

   Expression

   Plant

   Mutation

   miR160-resistant version of ARF17 has five silent…

   Promoter

   Available since

   June 2, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pEMS2156

   Plasmid

   #158099

   Purpose

   High Copy number plasmid containing SYN1 insert

   Depositor

   Elizabeth Simpson

   Article

   de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

   Insert

   SYN1 (SYN1 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   SYN1

   Available since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. OptoProfilin.S138E

   Plasmid

   #208287

   Purpose

   Expresses Cry2(1-531)-mCherry-Profilin.S138E

   Depositor

   Robert Hughes

   Article

   Mann et al Chembiochem. 2024 May 2;25(9):e202400007

   Insert

   Cry2 - mCherry - Profilin.S138E (Cry2, PRF1 Mouse, Mustard Weed)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Ser 138 (sometimes numbered 137) mutated to Glu

   Promoter

   CMV

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. OptoProfilin.S138A

   Plasmid

   #208288

   Purpose

   Expresses Cry2(1-531)-mCherry-Profilin.S138A

   Depositor

   Robert Hughes

   Article

   Mann et al Chembiochem. 2024 May 2;25(9):e202400007

   Insert

   Cry2 - mCherry - Profilin (Cry2, PRF1 Mouse, Mustard Weed)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Ser 138 (sometimes numbered 137) mutated to Ala

   Promoter

   CMV

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p202-Syt1-C2A-I248V

   Plasmid

   #201554

   Purpose

   Expresses Octopus synaptotagmin-1 C2A domain with I248V mutation in E. coli

   Depositor

   Joshua Rosenthal

   Article

   Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

   Insert

   Synaptotagmin-1 C2A domain (partial)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Isoleucine 248 to Valine (numbering based…

   Promoter

   RBS

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1-V5-HisB-ObKHC-K282R-HaloTag

   Plasmid

   #201552

   Purpose

   Expresses Octopus kinesin-1 motor with K282R mutation with HaloTag in Drosophila S2 cells

   Depositor

   Joshua Rosenthal

   Article

   Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

   Insert

   Kinesin-1
   

   Use

   Tags

   HaloTag

   Expression

   Insect

   Mutation

   changed lysine 282 to arginine (numbering based o…

   Promoter

   Ac5

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-lambdaN-HA-DmGW182-V5His6_H

    Plasmid

    #146499

    Purpose

    Insect Expression of DmGW182

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

    Insert

    DmGW182 (gw Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Two non silent mutations V704A and N799S and one …

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FUS_LC_withR

    Plasmid

    #139128

    Purpose

    express FUS LC with the same number and spacing of arginines as hnRNPA2 LC

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    add arginines

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pB6-Lrig1-T2A-tdTomato-FNF-TK

    Plasmid

    #134320

    Purpose

    B6J Lrig1-T2A-tdTomato allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    Lrig1 (Lrig1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    C-terminal fusion of T2A-tdTomato

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB6-Cdk6-T2A-td-sfGFP-FNF-TK

    Plasmid

    #134321

    Purpose

    B6J Cdk6-T2A-td-sfGFP allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    Cdk6 (Cdk6 Mouse)
    

    Use

    Mouse Targeting

    Tags

    C-terminal fusion of T2A-tandem dimer-superfolder…

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEMS1942

    Plasmid

    #105867

    Purpose

    High copy number plasmid containing Ple265 (PCP2 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    Ple265
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Ple265 (PCP2 MiniPromoter)

    Available since

    Feb. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pEMS2235

    Plasmid

    #111888

    Purpose

    High copy number plasmid containing Ple320 (POU4F1 MiniPromoter) insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple320 (POU4F1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Ple320 (POU4F1 MiniPromoter)

    Available since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pEMS2236

    Plasmid

    #111889

    Purpose

    High copy number plasmid containing Ple321 (TUBB3 MiniPromoter) insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple321 (TUBB3 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Ple321 (TUBB3 MiniPromoter)

    Available since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pEMS1930

    Plasmid

    #79779

    Purpose

    High copy number pUC vector containing Ple257 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple257
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple257 (PAX6 MiniPromoter)

    Available since

    Nov. 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pEMS1931

    Plasmid

    #79780

    Purpose

    High copy number pUC vector containing Ple258 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple258
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple258 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pEMS1929

    Plasmid

    #79778

    Purpose

    High copy number pUC vector containing Ple256 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple256
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple256 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pEMS1928

    Plasmid

    #79777

    Purpose

    High copy number pUC vector containing Ple255 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple255
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple255 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pEMS1927

    Plasmid

    #79776

    Purpose

    High copy number pUC vector containing Ple254 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple254
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple254 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pSG5-FLAG-mEKLF Zinc Fingers

    Plasmid

    #67837

    Purpose

    Expression of murine EKLF Zinc Fingers (aa 287-end) fused to Flag and driven by SV40 promoter

    Depositor

    James Bieker

    Article

    Chen et al Mol Cell Biol. 2001 May;21(9):3118-25.

    Insert

    EKLF (Klf1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Zinc Fingers are amino acids 287-end; this number…

    Promoter

    SV40

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

23. 5mARF17 pBluescript

    Plasmid

    #12069

    Depositor

    David Bartel

    Article

    Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

    Insert

    5mARF17 (ARF17 Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    miR160-resistant version of ARF17 has five silent…

    Promoter

    Available since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA3 Flag MFF iso2 S172A

    Plasmid

    #74392

    Purpose

    Flag tagged human S172A MFF isoform 2 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pET30a-His6-ABP-LOXCATmut

    Plasmid

    #139972

    Purpose

    A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAG416GAL-ccdB-mRuby3

    Plasmid

    #166139

    Purpose

    Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a CEN/ARS element for low copy number when in yeast.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRB051_LEU2

    Plasmid

    #196993

    Purpose

    Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

    Depositor

    Matthew Maurano

    Article

    Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.

    Type

    Empty backbone

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFUSEss-CHIg-mG1_Arg322Lys

    Plasmid

    #105849

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg322Lys (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

    Insert

    IgG1 heavy chain (Ighg1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Arg322Lys in constant region of IgG1 heavy chain

    Promoter

    hEF1-HTLV

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCS2-mkgDUX4mal*leu*

    Plasmid

    #21173

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Apr 9. ():.

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    First point mutation at nucleotide 5114 (numberin…

    Promoter

    Available since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

30. pCS2-mkgDUX4mal*mqg*

    Plasmid

    #21174

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Apr 9. ():.

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    First point mutation at nucleotide 5114 (numberin…

    Promoter

    Available since

    Aug. 6, 2009

    Availability

    Academic Institutions and Nonprofits only

31. pEMS1920

    Plasmid

    #82562

    Purpose

    High copy number plasmid containing Ple253 (PITX3 MiniPromoter) insert.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    Ple253
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pcDNA3 Flag MFF iso4 S172A

    Plasmid

    #74393

    Purpose

    Flag tagged human S172A MFF isoform 4 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

33. pEMS1941

    Plasmid

    #82564

    Purpose

    High copy number plasmid containing Ple264 (NR2E1 MiniPromoter) insert.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    Ple264
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pAG426GAL-ccdB-mRuby3

    Plasmid

    #166142

    Purpose

    Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a 2um element for high copy number when in yeast.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. HIS_TEV_hsSYX3

    Plasmid

    #179290

    Purpose

    E. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Use

    Tags

    His6 + TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    residues 393-792 from accession number NP_0010361…

    Promoter

    T7lac

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pX330_gRNA_H19-DMR-2

    Plasmid

    #159930

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CBh

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pX330_gRNA_H19-DMR-1

    Plasmid

    #159929

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CBh

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pB6-Lrig1-T2A-sfGFP-iCre-ERT2-FNF-TK

    Plasmid

    #126651

    Purpose

    B6J Lrig1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    Lrig1 (Lrig1 Mouse)
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    C-terminal fusion of T2A-sfGFP-iCre-ERT2

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 35S:5mARF17 pLBR19

    Plasmid

    #12073

    Depositor

    David Bartel

    Article

    Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

    Insert

    5mARF17 (ARF17 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    miR160-resistant version of ARF17 has five silent…

    Promoter

    Available since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

40. pTT5-hSDC1

    Plasmid

    #52326

    Purpose

    expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R117Q mutation (R95Q according to numbering in Fi…

    Promoter

    CMV

    Available since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge