We narrowed to 874 results for: PX330

Showing: 641 - 680 of 874 results

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pU6-LbCpf1-crR2

Plasmid

#213047

Purpose

Guide RNA expression vector with the synthetic crRNA crR2 targeting the SWITCHER construct with the target site TR2

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

crR2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLoxP-EGFP-crRNA-entry

Plasmid

#213048

Purpose

SWITCHER ready LoxP-EGFP reporter plasmid for cloning of a customized CRISPR-inducible Cre-construct

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

LoxP-EGFP-MALAT1-triplex

Use

CRISPR

Expression

Mammalian

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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C-13-BE

Plasmid

#135041

Purpose

C-T base editing using LpCDA1L1_4 fused to C terminal of nCas9

Depositor

Tian-Lin Cheng, Zilong Qiu

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L1_4

Use

CRISPR

Expression

Mammalian

Promoter

U6 with EF-1alpha intron

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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C-7-BE

Plasmid

#135035

Purpose

C-T base editing using LpCDA1L1_1 fused to C terminal of nCas9

Depositor

Tian-Lin Cheng, Zilong Qiu

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L1_1

Use

CRISPR

Expression

Mammalian

Promoter

U6 with EF-1alpha intron

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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C-6-BE

Plasmid

#135034

Purpose

C-T base editing using LjDA1L2_2 fused to C terminal of nCas9

Depositor

Tian-Lin Cheng, Zilong Qiu

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LjDA1L2_2

Use

CRISPR

Expression

Mammalian

Promoter

U6 with EF-1alpha intron

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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C-11-BE

Plasmid

#135039

Purpose

C-T base editing using LpCDA1L2_2 fused to C terminal of nCas9

Depositor

Tian-Lin Cheng, Zilong Qiu

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L2_2

Use

CRISPR

Expression

Mammalian

Promoter

U6 with EF-1alpha intron

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pSLHDR_R1

Plasmid

#124209

Purpose

gRNA/Cas9 expression for 2nd editing using pSLHDR01, 02

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Insert

gRNA

Use

CRISPR

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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7b sgRNA for EJ7-GFP reporter

Plasmid

#113624

Purpose

sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7b sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
psgRNA-GST-EGFP-GPIDAF

Plasmid

#213719

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Use

CRISPR

Tags

GST

Promoter

CMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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7i sgRNA for 4-μHOM reporter

Plasmid

#113626

Purpose

sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7i sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
7h sgRNA for 4-μHOM reporter

Plasmid

#113625

Purpose

sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7h sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
ER50-SpCas9-ER50

Plasmid

#85448

Purpose

Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

ER50-SpCas9-ER50

Use

AAV

Tags

destabilized domain of estrogen receptor ligand b…

Expression

Mammalian

Promoter

Cbh

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pREDIT_Cas9-MS2-BB_BbsI

Plasmid

#164802

Purpose

REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-TCAB1 KI sgRNA

Plasmid

#207611

Purpose

sgRNA for HaloTag insertion into the endogenous TCAB1 locus

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

CCAAAGTCTTCATACTGCAG

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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N-terminal Halo-XLF

Plasmid

#207546

Purpose

Homologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

HaloTag with internal PuroR cassette flanked by human Lig4 locus sequences

Tags

HaloTag

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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USP19 sgRNA2

Plasmid

#78586

Purpose

delete USP19 gene in human cell

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 sgRNA2 (USP19 Human)

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
N-Terminal Halo XRCC4

Plasmid

#207541

Purpose

Homologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

HaloTag with internal PuroR cassette flanked by human XRCC4 locus sequences

Tags

HaloTag

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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N-terminal Halo-Ku70

Plasmid

#207547

Purpose

Homologous recombination donor to insert halo tag at the N terminal of the endogenous Ku70 locus.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

HaloTag with flanked by human Ku70 locus sequences

Tags

HaloTag

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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sgRNA TR KO 2

Plasmid

#207610

Purpose

sgRNA 2 to knockout TR by replacement with a PuroR cassette

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

TCAGGCCGCAGGAAGAGGAA

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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psgRNA-GST-EGFP-GPIBY55

Plasmid

#213720

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 under a CMV promoter.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Use

CRISPR

Tags

GST

Promoter

CMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psgRNA-GST-EGFP-GPICEAM7

Plasmid

#213721

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 under a CMV promoter.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Use

CRISPR

Tags

GST

Promoter

CMV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA TR KO 1

Plasmid

#207609

Purpose

sgRNA 1 to knockout TR by replacement with a PuroR cassette

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

ACCCTAACTGAGAAGGGCGT

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
7a sgRNA for EJ7-GFP and 4-μHOM reporters

Plasmid

#113620

Purpose

sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7a sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
eSpCas9(1.1)_No_FLAG_AAVS1_T2

Plasmid

#79888

Purpose

Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

Untagged eSpCas9(1.1)

Expression

Mammalian

Promoter

CBh

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9.EF1a-BFP.sgLMNA

Plasmid

#98971

Purpose

Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNA and spCas9

Expression

Mammalian

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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DHFR-SpCas9-DHFR

Plasmid

#85447

Purpose

Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-SpCas9-DHFR

Use

AAV

Tags

Destabilized domain (C-terminus version) of E.col…

Expression

Mammalian

Promoter

Cbh

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-GFP-SFPQ-RT

Plasmid

#97090

Purpose

Encodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

EGFP-SFPQ-RT (SFPQ Human)

Use

CRISPR

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pX-evoCas9

Plasmid

#107550

Purpose

Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

Depositor

Anna Cereseto

Article

Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

Insert

Humanized S. pyogenes Cas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

M495V/Y515N/K526E/R661Q

Promoter

CBh

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pREDIT_Cas9n-MS2-BB_BbsI

Plasmid

#164804

Purpose

REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
HUWE1-sgRNA-1

Plasmid

#86924

Purpose

To express sgRNA target HUWE1 gene

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

HUWE1 sgRNA1 (HUWE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only
HUWE1-sgRNA-2

Plasmid

#86925

Purpose

To express sgRNA target HUWE1 gene

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

HUWE1 sgRNA2 (HUWE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only
pYFP-NEAT1m_v1-RT

Plasmid

#97089

Purpose

Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

NEAT1m_v1-RT (NEAT1 Human)

Use

CRISPR

Tags

EYFP

Promoter

CMV

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pX-EGFP-g1

Plasmid

#107273

Purpose

eGFP sgRNA-1 and Cas9 expression vector (aka. pX-ps1)

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Insert

GFP sgRNA-1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKC123

Plasmid

#134240

Purpose

expresses gRNA targeting CENP A 3'

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

CENP A 3' CRISPR construct

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Human SOX17 sgRNA

Plasmid

#59725

Purpose

Plasmid encoding sgRNA to generate SOX17 knock-out mutant human cells

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

hSox17 sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
Human BLIMP1 sgRNA

Plasmid

#59724

Purpose

Plasmid encoding sgRNA to generate BLIMP1 knockout mutant human cells

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

hBLIMP1 sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
pX-APRT-sg2

Plasmid

#107274

Purpose

APRT sgRNA-2 and Cas9 expression vector

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Insert

APRT sgRNA-2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
3xMS2 TR knockin sgRNA 1

Plasmid

#207607

Purpose

sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

cgactcgcccggcagcgcac

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
3xMS2 TR knockin sgRNA 2

Plasmid

#207608

Purpose

sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

acccccaaacctgactgact

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR/eCas9-GPIDAF

Plasmid

#213710

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Use

CRISPR

Tags

3XFLAG and Twin-strep-tag

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 874 results for: PX330