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Plasmid#64853PurposeCytosol-targeted version of the Ca2+ indicator GCaMP3.DepositorInsertGCaMP3
UseTagsNuclear export signal (NES)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Mito-ABKAR
Plasmid#61509PurposeExpression of AMPK biosensor targeted to the mitochondriaDepositorInsertAMPK Biosensor
UseTagsMitochondrial targeting signal (DAKAP1-30)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-Fast-1 wild-type
Plasmid#16521DepositorInsertFAST-1 (FOXH1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pP3-GFP
Plasmid#53436PurposeControls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genomeDepositorInsertP3 promoter
UseSynthetic BiologyTagsnoneExpressionBacterialMutationnonePromoterP3Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp YFP-PIF-SOS2cat
Plasmid#50851PurposeOptogenetic Ras activation system; cytoplasmic componentDepositorInsertsUseLentiviralTagsYFPExpressionMutationresidues 566–1049PromoterAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
aCKAR
Plasmid#124427PurposeFRET-based reporter for monitoring aPKC activity in cellsDepositorInsertaCKAR
UseTagsmCFP and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-ORF-V2
Plasmid#73638PurposeGateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).DepositorTypeEmpty backboneUseTagsVenus fragment 2 (V2)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-ORF-V1
Plasmid#73637PurposeGateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)DepositorTypeEmpty backboneUseTagsVenus fragment 1 (V1)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS-PesaRlux
Plasmid#47640Purposewt esaR promoter in pCS26DepositorInsertPesaR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPesaRAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
Calreticulin-SuperNova-KDEL
Plasmid#131857PurposeExpresses SuperNova fused to ER protein calreticulin and KDEL ER-retention signalDepositorInsertSuperNova
UseTagsKDEL ER-retention signal and Signal sequence of h…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp BFP-PIF-SOS2cat
Plasmid#50850PurposeOptogenetic Ras activation system; cytoplasmic componentDepositorInsertsUseLentiviralTagsTagBFPExpressionMutationresidues 566–1049PromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAP24
Plasmid#70190PurposeModular vector for secreted luciferase reporter expressionDepositorInsertsLucopt
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL101
Plasmid#22474PurposeExpresses APB domain of PIF6 fused to mYFP, for red-light-induced binding to PhyDepositorInsertPIF6APB
UseSynthetic BiologyTagsmYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta2
Plasmid#101290PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation1-727 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Golgi-ABKAR
Plasmid#61507PurposeExpression of AMPK biosensor targeted to the GolgiDepositorInsertAMPK Biosensor
UseTagsGolgi-targeting signal (see comments)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta1
Plasmid#101289PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation1-375 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-I
Plasmid#53437Purposexylose-dependent expression of the type-I agrC and agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX304-EV-IRES-GFP
Plasmid#187640PurposeEmpty Vector control modified to express GFPDepositorInsertEV
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pARR5::LUC
Plasmid#141170PurposeLuminescent reporter for cytokinin signalingDepositorInsertAtARR5 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtARR5, AT3G48100Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a
Plasmid#216385PurposeBaculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)DepositorInsertNorrin (NDP Human)
UseBaculovirusTagsGP64 signal sequence-MBP-3C and Rho1D4ExpressionInsectMutationC93A/C95A/C131APromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta3
Plasmid#101291PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation1-1080 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mito-SNAP
Plasmid#206480PurposeEncodes a mitochondrially-targeted SNAP tag for mammalian cell expressionDepositorInsertMitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)
UseTagsSNAPfExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAB18::LUC
Plasmid#141168PurposeLuminescent reporter for abscisic acid signalingDepositorInsertAtRAB18 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtRAB18, AT5G66400Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKar2-msGFP-HDEL
Plasmid#117668PurposeLocalisation of Endoplasmic ReticulumDepositorInsertpKar2-msGFP-HDEL
UseTagsExpressionYeastMutationContains the Kar2 promoter and signal sequence fr…PromoterpKar2Available sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIAA5::LUC
Plasmid#141169PurposeLuminescent reporter for auxin signalingDepositorInsertAtIAA5 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtIAA5, At1g15580Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCLX-M38s/TOP-dGFP
Plasmid#114295PurposeExpression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoterDepositorInsertdGFP
UseLentiviralTagsExpressionMutationdestabilized GFPPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Bov.hsc70.RSET.FL.wt(NarI)
Plasmid#12532DepositorInsertbovine 70 kDa heat shock cognate protein full length wild type
UseTagsExpressionBacterialMutationwild typePromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-I
Plasmid#53439PurposeT7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220DepositorInsertagrBD locus
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationnonePromoterT7-lacAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWKS1583
Plasmid#70188PurposeModular vector for secreted amylase reporter expressionDepositorInsertamyEopt
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta4
Plasmid#101292PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation76-1354 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta6
Plasmid#101294PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation375-1354 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAL189
Plasmid#22278PurposeExpresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitmentDepositorInsertIntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pKH-MoHyPer
Plasmid#78267PurposeExpress HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.DepositorInsertMoHyper
UseExpress in magnaportheTagsExpressionYeastMutationPromoterRp27Available sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASTattb-cAMPr Y205A, R739A
Plasmid#84933PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.DepositorInsertcAMPr Y205A R739A
UseTagsExpressionInsectMutationPKA-C Y205A, PKA-R R739APromoter5X UASAvailable sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASTattb-cAMPr Y205A, R626A
Plasmid#84932PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.DepositorInsertcAMPr Y205A R626A
UseTagsExpressionInsectMutationPKA-C Y205A, PKA-R R626APromoter5X UASAvailable sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta7
Plasmid#101295PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation727-1354 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-rhodopsin-CTS
Plasmid#86071PurposeA bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag.DepositorInsertRhodopsin (RHO Bovine)
UseTagsGSTExpressionBacterialMutationcontains the region from 309-348 of RhodopsinPromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV0764
Plasmid#133520PurposeExpression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0773
Plasmid#133519PurposeExpression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAZ10::LUC
Plasmid#141172PurposeLuminescent reporter for jasmonic acid signalingDepositorInsertAtJAZ10 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtJAZ10, AT5G13220Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_TH16_AVR-Pib_100
Plasmid#121378PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertTH16_AVR-Pib_100
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_TH12-rn_00121641
Plasmid#121371PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertTH12-rn_00121641
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_PH14-rn_00186401
Plasmid#121369PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertPH14-rn_00186401
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta5
Plasmid#101293PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation375-727 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-IV
Plasmid#53438Purposexylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWRKY70::LUC
Plasmid#141171PurposeLuminescent reporter for salicylic acid signalingDepositorInsertAtWRKY70 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtWRKY70, AT3G56400Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pISH-Galpha-o
Plasmid#15930DepositorInsertGalpha-o (Gnao1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-Fast-1 H83R
Plasmid#16522DepositorInsertFAST-1 H83R (FOXH1 Human)
UseTagsExpressionMammalianMutationH83R mutantPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCS2 YFP-Smo-Weyer's
Plasmid#41014DepositorInsertsmoothened homolog (Smo Mouse)
UseTagsEVC2 (aa 1177-1220) and YFPExpressionMammalianMutationYFP inserted after Smo signal sequence. Transfer…PromoterAvailable sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_CD156_AVR-Pib_70
Plasmid#121286PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertCD156_AVR-Pib_70
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only