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pEGFP-N1-C-Fzd6

Plasmid

#123589

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

frizzled class receptor 6 (Fzd6 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSRalpha-MSV-mouse Cdk4wt-Tk-neo

Plasmid

#115525

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Article

Matsushime et al Cell. 1992 Oct 16;71(2):323-34.

Insert

Mouse Cdk4 (Cdk4 Mouse)

Use

Retroviral

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only
pMK280 (mCherry2-NeoR)

Plasmid

#72796

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Bsr

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nrxn1beta AS4(-)-Fc

Plasmid

#59313

Purpose

Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

Depositor

Peter Scheiffele, Tito Serafini

Article

Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

Insert

Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)

Tags

Fc (from human IgG)

Expression

Mammalian

Promoter

CMV, T7

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLXSN IkB alpha M

Plasmid

#12330

Depositor

Inder Verma

Article

Van Antwerp et al Science. 1996 Nov 1. 274(5288):787-9.

Insert

IkB alpha M (Nfkbia Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Dominant-negative mutant. Many signal transductio…

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pmVenus-C2-MmMKL2

Plasmid

#67894

Purpose

Expresses a fusion of mVenus with MKL2 (MRTF-B) in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mVenus-MKL2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-Kv2.1 K+ channel [K89/34R-2b]

Plasmid

#199404

Purpose

Mammalian expression plasmid of Anti-Kv2.1 K+ channel [K89/34R-2b] (Rat). Derived from hybridoma K89/34.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Kv2.1 K+ channel (Rat) recombinant mouse monoclonal antibody (Kcnb1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9_U6_sgRNA_Mettl3C

Plasmid

#165420

Purpose

sgRNA construct for targeting Mettl3 C-terminus

Depositor

Neil Brockdorff

Article

Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

Insert

PspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flag-Mm Ezh2 Y641F

Plasmid

#80077

Purpose

Retroviral expression of mouse EZH2 Y641F mutant, hygromycin selection

Depositor

Phillip Abbosh

Inserts

Flag-EZH2 Y641F (Ezh2 Mouse)
Hygromycin phosphotransferase

Use

Retroviral

Tags

5' FLAG

Expression

Mammalian

Mutation

Y641F, R342R, N393N

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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Foxf1 HA pShuttle

Plasmid

#122120

Purpose

Expression of Foxf1 in mammalian cells

Depositor

Paul Herring

Article

Hoggatt et al J Biol Chem. 2013 Oct 4;288(40):28477-87

Insert

Foxf1 (Foxf1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Fgfr2-T2A-H2B-Halo donor

Plasmid

#113117

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Fgfr2 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-Ms-c-Myc-W136E

Plasmid

#26024

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

c-Myc (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

W136E

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pBabe HA-Runx1-neo

Plasmid

#45815

Depositor

Kevin Janes

Article

Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

Insert

Runx1 (Runx1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax

Plasmid

#190153

Purpose

Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp2 (Vamp2 Mouse)

Use

AAV

Tags

P2AT2A-EGFP-caax

Expression

Mammalian

Mutation

Truncation that includes only AA #1-94

Promoter

MBP

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Zeo-Sall4

Plasmid

#40797

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Sall4 (Sall4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
Dlx5

Plasmid

#15538

Depositor

John Rubenstein

Article

Zerucha et al J Neurosci. 2000 Jan 15. 20(2):709-21.

Insert

Dlx5 (Dlx5 Mouse)

Expression

Bacterial

Available Since

Aug. 20, 2007

Availability

Academic Institutions and Nonprofits only
TetO-FUW-Ezh2

Plasmid

#118587

Purpose

Expresses Ezh2 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Ezh2 (Ezh2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Hoxc6

Plasmid

#45553

Depositor

Jeremy Dasen

Article

Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

Insert

Hoxc6 (Hoxc6 Mouse)

Promoter

CMV / b-actin

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only
pLL7.0: mTiam1(64-437)-tgRFPt-SSPB R73Q

Plasmid

#60418

Purpose

Mammalian Expression of Tiam DH/PH-tgRFPt-Micro

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

mTiam1-tgRFPt-SSPB R73Q

Use

Cre/Lox and Lentiviral

Tags

tgRFPt

Expression

Mammalian

Mutation

mTiam 64-437 // SSPB R73Q, Y11K and A15E

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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PiggyBac-FLAG-mProser1-IRES-Blast

Plasmid

#226172

Purpose

Expresses full length mouse PROSER1 with 2X N-terminal FLAG tags

Depositor

Kasper Rasmussen

Article

Fleming et al Genes Dev. 2024 Nov 19. doi: 10.1101/gad

Insert

Proline Serine Rich protein 1 (Proser1 Mouse)

Tags

2X FLAG

Expression

Mammalian

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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