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We narrowed to 7,104 results for: GFP expression plasmids

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Showing: 6761 - 6800 of 7104 results

  2. pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)

    Plasmid
    #117168
    Purpose
    CRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    117168-plasmid-map-sequence-id-238529

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  6. pAAV-DYRK4-2A-mCherry

    Plasmid
    #118289
    Purpose
    Expresses mouse DYRK4 tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (Dyrk4 Mouse)
    Use
    AAV
    Tags
    2A peptide and mCherry
    Expression
    Mammalian
    Promoter
    CMV with beta global intron
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118289-plasmid-map-sequence-id-229468

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  11. Linked VLP NES

    Plasmid
    #182432
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS-NES
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182432-plasmid-map-sequence-id-360114

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  12. UbC-blasticidine-P2A-TETon-3G transactivator

    Plasmid
    #200191
    Purpose
    A TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    blasticidine - P2A - TETon-3G transactivator
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Promoter
    UbC
    Available Since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200191-plasmid-map-sequence-id-405986

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  15. deltaVP1 + VP2C-MIOX

    Plasmid
    #166680
    Purpose
    Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Inserts
    VP2C-MIOX
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available Since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    166680-plasmid-map-sequence-id-329927

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  16. FOR human OCT4 TALEN

    Plasmid
    #52412
    Purpose
    TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)
    Depositor
    Jacob Hanna
    Article
    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi
    Insert
    HD NG NN NN NN HD NG HD NG HD HD HD NI NG
    Use
    TALEN
    Promoter
    CAGGS
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pUt-mNF-BACH1 Cas9-Resistant

    Plasmid
    #199218
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    CMV D2ir promoter
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199218-plasmid-map-sequence-id-396470

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  19. TRUPATH Triple Gai2

    Plasmid
    #196049
    Purpose
    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196049-plasmid-map-sequence-id-385863

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  20. TRUPATH Triple Ga11

    Plasmid
    #196059
    Purpose
    Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha11-RLuc8 (GNA11 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196059-plasmid-map-sequence-id-386126

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  21. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196060-plasmid-map-sequence-id-385870

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  22. TRUPATH Triple GaZ

    Plasmid
    #196053
    Purpose
    Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaz-RLuc8 (GNAZ Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196053-plasmid-map-sequence-id-387469

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  25. pQE81L-KOFP7

    Plasmid
    #160330
    Purpose
    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.
    Depositor
    Sang Taek Jung
    Article
    Ko et al J Agric Food Chem. 2020 May 27;68(21):58
    Insert
    KOFP7
    Tags
    6xHis tag
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    160330-plasmid-map-sequence-id-326602

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  28. pOPS0383

    Plasmid
    #133233
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with NoP (pOGG113), sfGFP (pOGG037) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    April 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    133233-plasmid-map-sequence-id-287536

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  38. pOPS0381

    Plasmid
    #133231
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133231-plasmid-map-sequence-id-282538

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  39. pASPIre3

    Plasmid
    #154844
    Purpose
    Derivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this study
    Depositor
    Markus Jeschek
    Article
    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:
    Insert
    Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
    Expression
    Bacterial
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    154844-plasmid-map-sequence-id-307642

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Showing: 6761 - 6800 of 7104 results