We narrowed to 12,882 results for: LIC

1. pIRES2-eGFP:HA-mTREK-1(S131C/A286F)

   Plasmid

   #224775

   Purpose

   Mammalian expression vector for generating mouse TREK-1

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S131C / A286F

   Promoter

   CMV

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pIRES2-eGFP:HA-mTREK-1(tandem wt-S131C)

   Plasmid

   #224778

   Purpose

   Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Tandem TREK-1 wt-S131C) (Kcnk2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S562C

   Promoter

   CMV

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pIRES2-eGFP:HA-mTREK-1(tandem wt-wt)

   Plasmid

   #224776

   Purpose

   Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Tandem TREK-1 (wt-wt) (Kcnk2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEMHE:mTREK-1(tandem S131C-S131C)

   Plasmid

   #224760

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-S131C)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Tandem TREK-1 (S131C-S131C) (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   S131C / S562C

   Promoter

   T7

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEMHE:mTREK-1 (S131A/C159A/C219A)

   Plasmid

   #224753

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   S131A/C159A/C219A

   Promoter

   T7

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEMHE:mTREK-1 (S131C/C159A/C219A)

   Plasmid

   #224752

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   S131C/C159A/C219A

   Promoter

   T7

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEMHE:mTREK-1 (C159A/C219A/C365A/C399A)

   Plasmid

   #224754

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A/C365A/C399A)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   C159A/C219A/C365A/C399A

   Promoter

   T7

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBK1301-AAV-EFSNC-dCjCas9-NIPP1(143-224)

   Plasmid

   #223146

   Purpose

   Expression of truncated NIPP1 with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   NIPP1 (PPP1R8 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 143-224

   Promoter

   EF1a

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBK1311-AAV-EFSNC-dSaCas9-NIPP1(143-224)

   Plasmid

   #223156

   Purpose

   Expression of truncated NIPP1 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   NIPP1 (PPP1R8 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 143-224

   Promoter

   EF1a

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO.1 mTagBFP2 rat DJ-1 shRNA

    Plasmid

    #222870

    Purpose

    Expresses mTagBFP2 along with an shRNA against rat DJ-1

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    DJ-1 shRNA (Park7 Rat)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmcherry-mTet1s pc3905

    Plasmid

    #201703

    Purpose

    Expresses of mcherry-tagged mTet1s in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    Tet1s (Tet1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGM84

    Plasmid

    #220172

    Purpose

    CRISRPi guide RNA against BTF3

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    BTF3 (sgRNA) (BTF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pC1_oROS-HT_LF(C199S)

    Plasmid

    #216412

    Purpose

    Expresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT_LF(C199S)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pC1-lifeact-oROS-HT

    Plasmid

    #216420

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    lifeact-oROS-HT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS-AnkG ZU5in-3xHA

    Plasmid

    #213730

    Purpose

    Mammalian cell expression vector for overexpression of AnkG ZU5in isoform in motor neurons.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    AnkG ZU5in (Ank3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-AnkB ZU5ex-3xHA

    Plasmid

    #213732

    Purpose

    Mammalian cell expression vector for overexpression of AnkB ZU5ex isoform in motor neurons.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    AnkB ZU5ex (Ank2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS-AnkG ZU5ex-3xHA

    Plasmid

    #213731

    Purpose

    Mammalian cell expression vector for overexpression of AnkG ZU5ex isoform in motor neurons.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    AnkG ZU5ex (Ank3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-Dlx-SynaptoTAG

    Plasmid

    #210729

    Purpose

    AAV vector for mapping synaptic projections. GABAergic neuron-specific Dlx promoter expresses EGFP-Synaptobrevin-2 to label synaptic terminals and Palm-tdTomato to label neuronal soma and axons.

    Depositor

    Wei Xu

    Article

    Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

    Insert

    EGFP-Synaptobrevin-2, P2A, Palm-tdTomato (tdTomato with Palmitoylation sequence) (Vamp2 Rat, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Dlx

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)

    Plasmid

    #208977

    Purpose

    Entry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)

    Plasmid

    #208974

    Purpose

    Recruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    B103-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

    Plasmid

    #208971

    Purpose

    Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(-exo;D169A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

    Plasmid

    #208970

    Purpose

    Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29(+exo)-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-CHMP2A-myc, L216D/L219D

    Plasmid

    #180645

    Purpose

    Mammalian expression of CHMP2A with L216D/L219D mutations. Has C-terminal Myc tag. Internal ID:WISP20-13.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    L216D, L219D

    Promoter

    pCAG

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG-OSF-KATNA1 R14A

    Plasmid

    #160052

    Purpose

    Mammalian expression vector for KATNA1 (KATANIN-P60) R14A mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-19.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Tags

    One-Strep-Flag (OSF)

    Expression

    Mammalian

    Mutation

    R14A mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-OSF-KATNA1 V55D

    Plasmid

    #160053

    Purpose

    Mammalian expression vector for KATNA1 (KATANIN-P60) V55D mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-17.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Tags

    One-Strep-Flag (OSF)

    Expression

    Mammalian

    Mutation

    V55D mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCA528 KATNA1 MIT residues 1-79, V55D

    Plasmid

    #180615

    Purpose

    Bacterial expression for KATNA1 MIT domains, residues 1-79. Has V55D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-23.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-79, V55D mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCas-Cas12m-ΔZF

    Plasmid

    #192276

    Purpose

    Encodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    Cas12m ΔZF
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    H459A, C552A

    Promoter

    PJ23108

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBactin-Nedd1-mOrange2

    Plasmid

    #196860

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2 (Nedd1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-rox-inv[Talpha1-iCre-pA]-rox-LynGFP

    Plasmid

    #196874

    Purpose

    Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-Dre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    LynGFP
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-lox-inv[Talpha1-Dre-pA]-lox-Lyn-EGFP

    Plasmid

    #196876

    Purpose

    Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-iCre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    LynEGFP
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]

    Plasmid

    #196883

    Purpose

    Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-ZsGreen-bGH
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAG-DIO-inv[roxSTOProx-Lyn-GFP-bGH]

    Plasmid

    #196884

    Purpose

    Dual Cre/Dre-dependent expression of LynGFP. Used as Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-Lyn-GFP-bGH
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBactin-DIO-inv[roxSTOProx-ZsGreen-bGH]

    Plasmid

    #196886

    Purpose

    Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-ZsGreen-bGH
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGBT9-ATG9A-N

    Plasmid

    #198530

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-ATG9A-N terminal cytosolic domain fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

    Insert

    ATG9A N-terminal cytosolic tail (residues 1-66) (ATG9A Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBridge-tyrosinase.σ3A

    Plasmid

    #197513

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-3 σ3A
    

    Use

    Tags

    GAL4-DNA binding domain fragment, HA tag, and nuc…

    Expression

    Yeast

    Mutation

    silent substitution in codon 2 of tyrosinase tail…

    Promoter

    ADH1 and MET25

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pROS_phleo-Tps1/Gsy2

    Plasmid

    #196612

    Purpose

    Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    TPS1, GSY2 (TPS1, GSY2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCIneo-V5-SBP-MBP-HsNot9-V181E-shRNA2res_AG

    Plasmid

    #148829

    Purpose

    Mammalian Expression of HsNot9-V181E-shRNA2res

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

    Insert

    HsNot9-V181E-shRNA2res (CNOT9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pnEA-HsNot10_25-707-HsNot11_257-498-CvH_AF

    Plasmid

    #148731

    Purpose

    Bacterial Expression of HsNot10_25-707-HsNot11_257-498

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

    Insert

    HsNot10_25-707-HsNot11_257-498 (CNOT10 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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39. pnEA-NpG-HsNot9_19-285_AF

    Plasmid

    #148733

    Purpose

    Bacterial Expression of HsNot9_19-285

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

    Insert

    HsNot9_19-285 (CNOT9 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pROS13-Tps2/Gsy1

    Plasmid

    #196613

    Purpose

    Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    S000001911 (TPS2, GSY1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pUGP1.1

    Plasmid

    #196615

    Purpose

    Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
    

    Use

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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42. pAAV-hSyn-fDIO-mScarlet-Gphn_C4a

    Plasmid

    #194973

    Purpose

    AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4a) under the control of human Synapsin promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    mScarlet-Gphn (isoform C4a) (Gphn Rat, Synthetic)
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAAV-hSyn-fDIO-mScarlet-Gphn_C4d

    Plasmid

    #194975

    Purpose

    AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4d) under the control of human Synapsin promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    mScarlet-Gphn (isoform C4d) (Gphn Rat, Synthetic)
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAAV-hSyn-fDIO-mEGFP-Gphn_P1

    Plasmid

    #194978

    Purpose

    AAV vector to drive the Flp-dependent expression of mEGFP (L221K) -Gphn (isoform P1) under the control of human Synapsin promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    mEGFP-Gphn (isoform P1) (Gphn Rat, Synthetic)
    

    Use

    AAV

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pAAV-hSyn-fDIO-mScarlet-Gphn_P1

    Plasmid

    #194972

    Purpose

    AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform P1) under the control of human Synapsin promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    mScarlet-Gphn (isoform P1) (Gphn Rat, Synthetic)
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAAV-hSyn-fDIO-mScarlet-Gphn_C4c

    Plasmid

    #194974

    Purpose

    AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4c) under the control of human Synapsin promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    mScarlet-Gphn (isoform C4c) (Gphn Rat, Synthetic)
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. mKate2-DrCav1 in pT3TS-Dest

    Plasmid

    #194293

    Purpose

    In vitro transcription of mKate2 tagged zebrafish caveolin1 from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone KZW

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    caveolin (cav1.S Frog)
    

    Use

    In vitro transcription of mrna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. p3E-DrCavin1a

    Plasmid

    #194291

    Purpose

    Multisite gateway entry clone for expression of codon optimised zebrafish cavin1a with fusion tag at the N-terminus. Parton lab clone KXM

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    cavin1a (cavin1a Zebrafish)
    

    Use

    Multisite gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. mRFP-PA-GFP-RhoAQ63L

    Plasmid

    #187282

    Purpose

    Express pmRFP-PAGFP-RhoA Q63L

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    mRFP-PAGFP

    Expression

    Mammalian

    Mutation

    RhoA Q63L

    Promoter

    CMV, T7

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pET21a Sa-iPGM-bio

    Plasmid

    #179523

    Purpose

    Expresses S. aureus iPGM containing a C-terminal biotinylation site and His tag in E. coli

    Depositor

    James Inglese

    Article

    van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

    Insert

    S. aureus iPGM bio-6His
    

    Use

    Tags

    His tag and biotinylation sequence

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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