We narrowed to 10,333 results for: SEC
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Plasmid#65269Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertIi-Streptavidin and truncated sialyltransferase (ST6GAL1 Human)
TagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDragon-empty
Plasmid#155018PurposeEmpty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.DepositorTypeEmpty backboneUseMouse TargetingAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGLucIPZ
Plasmid#228703PurposeConstitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interestDepositorInsertsGaussia luciferase
non-silencing shRNA
UseLentiviralExpressionMammalianAvailable SinceDec. 2, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMX Vav1 Y3F uGFP
Plasmid#14558DepositorInsertVav1 (VAV1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationY3F (Y142, Y160, Y174) in DH domainAvailable SinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
ST-SBP-mCherry_puromycin
Plasmid#65271PurposeST reporter only ( no hook) (RUSH system)DepositorInserttruncated sialyltransferase (ST6GAL1 Human)
TagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYSD300
Plasmid#212930PurposeEncodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.DepositorInsertAGA1 (AGA1 Budding Yeast)
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV eIF4E W73A IRES GFP
Plasmid#18762DepositorAvailable SinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUC57-GFP-SFPQ-RT
Plasmid#97090PurposeEncodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.DepositorInsertEGFP-SFPQ-RT (SFPQ Human)
UseCRISPRAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal NLS
Plasmid#188396PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptide and SV40 NLSExpressionBacterialPromoterPhyper spankAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB088h
Plasmid#79215PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-TnCAD-F3
ExpressionBacterialPromoterPlacZ-opt (OR1+2); Ppro1Available SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-tdTomato-FlagAkt2
Plasmid#64832PurposeLentiviral expression of murine Flag-Akt2DepositorAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
polyPRM5
Plasmid#175232PurposeBacterial expression and purification, mixed with polySH3 to form condensatesDepositorAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDragon-DTA
Plasmid#155017PurposeFor Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to attenuated diphtheria toxin (tox176) in the presence of Cre and (r)tTA activityDepositorInsertDTA
UseMouse TargetingMutationtox176Available SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
RIPR-bio-His
Plasmid#50824PurposeExpresses enzymatically monobiotinylated full length RIPRDepositorInsertRIPR
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ST-SBP-tagBFP
Plasmid#65266Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-KDEL and truncated sialyltransferase (ST6GAL1 Human)
TagsStreptavidin Binding Protein (SBP) and tagBFPExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable SinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-b (p15A origin)
Plasmid#74089PurposeSecondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB078d9
Plasmid#79207PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1+2+3); Ppro1Available SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP3
Plasmid#135552PurposeExpress rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP3
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSDest-MYC-TMPRSS2
Plasmid#154964PurposeTMPRSS2 with N-terminal MYCDepositorAvailable SinceJuly 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1
Plasmid#46676DepositorInserthsa-mir-133a-1 (MIR133A1 Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianPromoterCMVAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only