We narrowed to 14,039 results for: TIM

1. FLAG-TRAF6-wt

   Plasmid

   #21624

   Depositor

   John Kyriakis

   Article

   Zhong et al Mol Cell Biol. 2004 Oct . 24(20):9165-75

   Insert

   TNF receptor-associated factor 6 (Traf6 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

   Plasmid

   #175523

   Purpose

   Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   LAMP1-HaloTag11 and Tomm20-HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCSF-R-luc

   Plasmid

   #12420

   Depositor

   Dong-Er Zhang

   Article

   Zhang et al Mol Cell Biol. 1994 Jan . 14(1):373-81.

   Insert

   M-CSF receptor promoter fragment (CSF1R Human)
   

   Use

   Luciferase

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pN256

   Plasmid

   #188472

   Purpose

   scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

   Depositor

   Le Cong

   Article

   Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

   Insert

   eGFP-WPRE-DAISY-4T
   

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

   Plasmid

   #163076

   Purpose

   Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   mito-roGFP2-Orp1
   

   Use

   Gateway entry vector

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-HsSENP1

   Plasmid

   #71465

   Purpose

   Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 1 (SENP1 Human)
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

   Plasmid

   #20943

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CaMKIIa

   Available Since

   July 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

   Plasmid

   #138989

   Purpose

   IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

   Depositor

   Jeff Hammerbacher

   Article

   Gottschalk et al bioRxiv 2021.11.30.470597

   Insert

   TCRA (Tcra Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

   Plasmid

   #175525

   Purpose

   Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag10 and Tomm20-HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. XE118 CAMKII-T286D-CS2+

    Plasmid

    #16736

    Depositor

    Randall Moon

    Article

    Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

    Insert

    CAMKII-T286D (Camk2a Rat)
    

    Use

    Xenopus expression

    Mutation

    threonine 286 mutated to aspartate

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

11. Intron-Tagging-EGFP-Donor

    Plasmid

    #159740

    Purpose

    Contains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

    Insert

    EGFP
    

    Use

    Intron tagging donor

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PTP1B human

    Plasmid

    #102719

    Purpose

    human PTP1B catalytic domain (residues 1-301) in RP1B vector

    Depositor

    Wolfgang Peti

    Article

    Choy et al Mol Cell. 2017 Feb 16;65(4):644-658.e5.

    Insert

    Protein Tyrosine Phosphatase 1B (PTP1B) (PTPN1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP

    Plasmid

    #169332

    Purpose

    Nuclear expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag9 (H2BC21 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEB1-moxCerulean3

    Plasmid

    #103975

    Purpose

    Plasmid encoding moxCerulean3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    moxCerulean3
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, Y66W, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-CLIP-PD-L2

    Plasmid

    #223587

    Purpose

    Lentiviral expression of human PD-L2 with CLIP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    PD-L2 (PDCD1LG2 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    CLIP

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-MSP1D1deltaH4-H6

    Plasmid

    #71717

    Purpose

    Membrane scaffold protein (MSP), helix 4 to 6 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Expression

    Bacterial

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

    Plasmid

    #185918

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

    Plasmid

    #180267

    Purpose

    Lentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNA

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    EGFR sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGF-mSox2-ONL

    Plasmid

    #65719

    Purpose

    mSox2 reporter plasmid (expresses ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    murine Sox2 promoter and ONL
    

    Use

    Lentiviral and Luciferase

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-mBlueberry2-P2A-EGFP

    Plasmid

    #184937

    Purpose

    Expresses blue fluorescent protein mBlueberry2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    mBlueberry2-P2A-EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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