We narrowed to 10,723 results for: SEC
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Plasmid#70218PurposeExpresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.DepositorInsertGFP11x7-mCherry-α-tubulin
TagsGFP11x7 and mCherryExpressionInsectAvailable SinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-Munc18-1wt
Plasmid#135550PurposeExpress rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertMunc18-1wt
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mucin MUC1-5TR-pET15b
Plasmid#174694PurposePlasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)DepositorInsertMUC1-TR
Tagshexahistidine (HIS) tagExpressionBacterialMutationnonePromoterT7 PromoterAvailable SinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB088c
Plasmid#79213PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-TnTBR3-F3
ExpressionBacterialPromoterPlacZ-opt (OR1); Ppro1Available SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLIB-HIS-TEV-scCAK1
Plasmid#177014PurposeGenerate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEVDepositorInsertCAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
TagsPolyhistidine-TEVExpressionInsectAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-Cdc42-2G
Plasmid#68814PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)DepositorInsertCdc42 activation reporter
TagsHis and MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWSK129-stm2585
Plasmid#174401PurposeS. Typhimurium stm2585 (sarA/steE) flanked with endogenous regulatory sequences (786bp on 5' and 28bp on 3' end) for bacterial expression.DepositorInsertsarA
ExpressionBacterialAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ACE2-GFP
Plasmid#154962PurposepcDNA3.1-ACE2-GFPDepositorAvailable SinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYSD099
Plasmid#212923PurposeA MoClo Yeast Toolkit LEU2, CEN/ARS empty vector (GFP dropout). Contains ConL1, part234 GFP dropout, ConR2, LEU2, CEN6/ARS4, AmpR-ColE1.DepositorInsertGFP
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-hM3Dq-mCherry
Plasmid#166599PurposeEncodes Cre-dependent hM3Dq-mCherry under control of the TREDepositorInserthM3Dq-mCherry
UseAAVPromotertetracycline response elementAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYSD300
Plasmid#212930PurposeEncodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.DepositorInsertAGA1 (AGA1 Budding Yeast)
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD153
Plasmid#212929PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD141
Plasmid#212928PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYFP-NEAT1pr_v2-RT
Plasmid#97088PurposeEncodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v2 vector, but also works with px330-NEAT1pr_v1.DepositorAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
RINS1(mut)
Plasmid#107291PurposePlasmid for the expression of the inactive mutant of RINS1 sensorDepositorInsertRINS1(mut)
TagssfGFPExpressionMammalianMutationtwo Arg/Ser mutations at the beginning and in the…PromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4EHP-W114A_AH
Plasmid#148909PurposeInsect Expression of Dm4EHP-W114ADepositorInsertDm4EHP-W114A (4EHP Fly)
ExpressionInsectMutation4 silent mutations compared to the sequence given…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH_Str-KDEL_ManII-SBP-EGFP
Plasmid#65258Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
UseLentiviralTagsEGFP and Streptavidin Binding Protein (SBP)Mutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable SinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PARP2-Flag-His-WT
Plasmid#111574PurposeExpresses full length PARP2 in Sf9 (insect) cellsDepositorInsertPARP2 (PARP2 Human)
TagsC-terminal 6xHis and C-terminal FlagExpressionInsectPromoterpolyhedrin promoterAvailable SinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIZ-Flag6His-BmAgo3
Plasmid#50559PurposeExpresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cellsDepositorInsertBmAgo3
Tags6xHIS and FlagExpressionInsectPromoterOpIE2Available SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCLucIPZ
Plasmid#53222PurposeConstitutive expression of Cypridina luciferase, puromycin N-acetyl-transferase and shRNAmir of interestDepositorInsertsCypridina luciferase
non-silencing shRNA
UseLentiviralExpressionMammalianAvailable SinceJune 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits