We narrowed to 18,336 results for: met

1. mRuby2-SiT-N-15

   Plasmid

   #55912

   Purpose

   Localization: Golgi TGN, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   SiT (ST6GAL1 Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   aa 1-45 of SiT

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAAVf-EnhCB-lacZnls miR-122 3x

   Plasmid

   #35644

   Depositor

   Phillip Zamore

   Article

   Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

   Insert

   3 miR-122 target sites
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CB

   Available Since

   April 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSFDuet-1_VNp-mNeongreen

   Plasmid

   #182387

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-mNeongreen-His
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP

   Plasmid

   #31481

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 1.1 (Sce target) EF BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   mCherry contains M9S and M16L to reduce backgroun…

   Promoter

   Available Since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. dCas9-10xSunTag(22aa)

   Plasmid

   #215745

   Purpose

   Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   dCas9-10xSunTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken beta-actin promoter

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG_mRuby2_smFP OLLAS

   Plasmid

   #59761

   Purpose

   encodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   mRuby2_smFP_OLLAS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAVS1-Nst-CAG-DEST

   Plasmid

   #80489

   Purpose

   Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVUHshp53

   Plasmid

   #11653

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   GFP, shRNA against TP53 (TP53 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEB2-E2-Crimson

   Plasmid

   #104010

   Purpose

   Plasmid encoding E2-Crimson

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   E2-Crimson
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. mKO2-SLBP(18-126)

    Plasmid

    #83914

    Purpose

    Fluorescent probe for S/G2 cell cycle transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mKO2-SLBP(18-126) (SLBP Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-CT3G-CERP2-MG-IRES2-mNeonGreen

    Plasmid

    #175508

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-UMI

    Plasmid

    #222694

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Promoter

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]

    Plasmid

    #74101

    Purpose

    vector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015

    Depositor

    Ben Szaro

    Article

    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

    Inserts

    Green Lantern Green Fluorescent Protein
    Rabbit beta 1-globin gene 3'UTR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2

    Plasmid

    #87694

    Purpose

    Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    ERT2-PhoCl-Cre-PhoCl-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. piGECI-N1

    Plasmid

    #160421

    Purpose

    Cytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECI

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH_Str-KDEL_ManII-SBP-mCherry

    Plasmid

    #65259

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Use

    Lentiviral

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas9 fused to nanobody (anti-beta catenin)

    Plasmid

    #186420

    Purpose

    Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    dCas9-nanobody fusion (anti-beta catenin)
    

    Use

    CRISPR

    Tags

    6His

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUCXMG

    Plasmid

    #204409

    Purpose

    Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site

    Depositor

    David Ackerley

    Article

    Rich et al bioRxiv. 2023 Mar 25:2023.03.24.534183.

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. peCALWY-4

    Plasmid

    #22236

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-4 Zn(II) sensor (ATOX1 Human, GFP's)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C418S mutation linker consists of 9 GGSGGS repea…

    Promoter

    Available Since

    Nov. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM6-vioABECD

    Plasmid

    #66535

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Consensus T7

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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