159,332 results

1. pH-nCas9-PBE

   Plasmid

   #98163

   Purpose

   Expresses CRISPR base editor in rice

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-nCas9-UGI-NLS
   

   Use

   Rice

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX303-ZIM3-KRAB-dCas9

   Plasmid

   #154472

   Purpose

   Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ZIM3 (ZIM3 Synthetic)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Includes ZIM3 aa 1-100

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-3xFLAG-BioID2-MCS

   Plasmid

   #186811

   Purpose

   CAG promoter-driven expression of BioID2-fused protein (N terminus)

   Depositor

   Masahito Ikawa

   Article

   Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

   Type

   Empty backbone

   Use

   Tags

   3xFLAG-BioID2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-hCDC6p-Venus-puro

   Plasmid

   #212666

   Purpose

   Express Venus under CDC6 promoter

   Depositor

   Yumi Konagaya, Tobias Meyer

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   hCDC6p-Venus
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hCDC6p

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-U6-gRNA scaffold-UbC-mCherry-PuroR

   Plasmid

   #232183

   Purpose

   Lentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherry

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, hUbC

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJ4M/TDP-43

   Plasmid

   #104480

   Purpose

   bacterial expression of TDP-43

   Depositor

   Nicolas Fawzi

   Article

   Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   MBP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-Tet-OsTIR1(F74G)-V5

   Plasmid

   #158664

   Purpose

   Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   Tet-OsTIR1(F74G) AAVS1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   F74G

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. LZRS-DNMT1

   Plasmid

   #24952

   Depositor

   Paul Khavari

   Article

   Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

   Insert

   DNMT1 (DNMT1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYC1640

   Plasmid

   #158719

   Purpose

   CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA4TO-24xGCN4_v4-kif18b-24xPP7

    Plasmid

    #74928

    Purpose

    Translation reporter

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    24xGCN4_v4-kif18b-24xPP7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSFFV_Gal4-VP64-IRES-mCherry (pLZA139)

    Plasmid

    #203909

    Purpose

    Encodes wild type Gal4-VP64 transcription factor in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    Gal4-VP64-IRES-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pY094

    Plasmid

    #84743

    Purpose

    Expresses huAsCpf1-T2A-GFP and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huAsCpf1
    GFP
    

    Use

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC

    Plasmid

    #221359

    Purpose

    Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC; to be used with Anti-V5 [IPI-SV5-Pk1] light chain (Plasmid 221360) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDONR223-MERTK

    Plasmid

    #23900

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MERTK (MERTK Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX neo PFKP-GFP

    Plasmid

    #116940

    Purpose

    Expression of PFKP-GFP fusion protein

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    PFKP (PFKP Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AICSDP-4:TUBA1B-mEGFP

    Plasmid

    #87421

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    TUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. mCherry-Ezrin-N-14

    Plasmid

    #55043

    Purpose

    Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Ezrin (EZR Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET_mNG2(1-10)_32aalinker_mNG2(11)

    Plasmid

    #82611

    Purpose

    Bacterial expression vector for split mNeonGreen2 screening

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    split before mutations

    Promoter

    T7

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1-blast

    Plasmid

    #26655

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. RET-WT

    Plasmid

    #125252

    Purpose

    Expresses human RET in mammalian cells

    Depositor

    Sissy Jhiang

    Article

    Xing et al Endocrinology. 1996 May;137(5):1512-9. d

    Insert

    RET (RET Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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