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We narrowed to 10,458 results for: T7

Showing: 6881 - 6920 of 10458 results

  6. pcDNA3-ABKAR-Kras

    Plasmid
    #84637
    Purpose
    Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, C…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    84637-plasmid-map-sequence-id-176126

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  17. pCDF-NtXNtT5R1AtR2NtB2

    Plasmid
    #160888
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-T5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    PT7-Nt-rbcS-T5
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-T5: T7, chaperones: T11
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160888-plasmid-map-sequence-id-318861

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  18. pCDF-NtXNtT1R1AtR2NtB2

    Plasmid
    #160885
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-T1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    PT7-Nt-rbcS-T1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-T1: T7, chaperones: T8
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160885-plasmid-map-sequence-id-318848

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  25. pAL950

    Plasmid
    #119748
    Purpose
    precursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Internal NdeI site removed and mutations Alanine …
    Promoter
    T7
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119748-plasmid-map-sequence-id-232481

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  26. pcDNA3-mCherry-PKAsub-RLuc8N-NES

    Plasmid
    #138211
    Purpose
    Encodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    mCherry-PKAsub-RLuc8N-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    138211-plasmid-map-sequence-id-307413

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  28. pMSP1D1T1

    Plasmid
    #97327
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1T1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of T residue after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97327-plasmid-map-sequence-id-182625

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  29. pMSP1D1T2

    Plasmid
    #97328
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1T2
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of two T residues after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97328-plasmid-map-sequence-id-182618

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  30. pET28-his-3C_Clu3B6p

    Plasmid
    #100093
    Purpose
    expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide
    Depositor
    Nikolai Sluchanko
    Article
    Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10
    Insert
    14-3-3 sigma human (SFN Human)
    Use
    Tags
    HSPB6 peptide and His6
    Expression
    Bacterial
    Mutation
    75-77 residues changed to AAA to facilitate cryst…
    Promoter
    T7
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100093-plasmid-map-sequence-id-193872

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  33. Cenp-B DBD INCENP TSS/AAA mCherry

    Plasmid
    #45234
    Purpose
    Target INCENP (with TSS mutated to AAA) to centromeres via CENP-B DNA binding domain (DBD)
    Depositor
    Michael Lampson
    Article
    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.
    Insert
    Cenp-B DBD, INCENP-ΔCEN with AAA mutation, mCherry
    Use
    Tags
    CENP-B DBD, DNA binding domain of CENP-B and mChe…
    Expression
    Mammalian
    Mutation
    INCENP C-terminal TSS mutated to AAA
    Promoter
    t7
    Available since
    Sept. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    45234-plasmid-map-sequence-id-84485

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Showing: 6881 - 6920 of 10458 results