Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 24,269 results for: fus

Clear keywords
Showing: 6881 - 6920 of 24269 results

  1. SL541

    Plasmid
    #49950
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD RH-7 (RHOXF2 Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  2. SL540

    Plasmid
    #49949
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD RH-6 (RHOXF2 Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. pSHUT4-eYFP

    Plasmid
    #133094
    Purpose
    Agrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHI
    Depositor
    Jens Laurids Sørensen
    Article
    Nielsen et al Fungal Biol Biotechnol. 2019 Dec 11;6:25
    Insert
    enhanced yellow flourescent protein
    Use
    Fungal expression
    Promoter
    TEF-1α
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133094-plasmid-map-sequence-id-266874

    Enlarge

  9. pQE80L-SpyCatcher-ELP-GFP

    Plasmid
    #69835
    Purpose
    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do
    Insert
    SpyCatcher-ELP-GFP
    Tags
    6x His Tag, GFP, and TEV Tag
    Expression
    Bacterial
    Promoter
    T5 promoter/lac operator element
    Available Since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    69835-plasmid-map-sequence-id-183106

    Enlarge

  10. pET-TRSter

    Plasmid
    #78865
    Purpose
    Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element
    Depositor
    Elias Arnér
    Article
    Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.
    Insert
    Thioredoxin Reductase (Txnrd1 Rat)
    Tags
    Mutated E. coli-type SECIS element (3’ end of ins…
    Expression
    Bacterial
    Promoter
    T7lac
    Available Since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    78865-plasmid-map-sequence-id-147493

    Enlarge

  12. pR I-Ab beta Derp1-117

    Plasmid
    #113639
    Purpose
    Insect cell expression vector for I-Ab beta chain fused to Der p 1 117-127 antigenic peptide
    Depositor
    James Moon
    Article
    Moon et al Methods Mol Biol. 2018;1799:165-181. doi
    Insert
    I-Ab beta Derp1-117 (H2-Ab1 Mouse)
    Tags
    6x His tag, fused to Der p 1 (117-127) peptide vi…
    Expression
    Insect
    Mutation
    transmembrane and cytoplasmic domains truncated
    Promoter
    Metallothionein
    Available Since
    Sept. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    113639-plasmid-map-sequence-id-221159

    Enlarge

  15. pET-EIN-AlkBH5(66-292)

    Plasmid
    #125732
    Purpose
    Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.
    Depositor
    Vincenzo Venditti
    Article
    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055
    Insert
    AlkBH5(66-292) (ALKBH5 Human)
    Tags
    6xHis and EIN
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125732-plasmid-map-sequence-id-244619

    Enlarge

  19. EBFP2-Cx26

    Plasmid
    #69021
    Purpose
    Expresses rat Cx26 (Gjb2 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Gja2 (Gjb2, Cx26 Rat)
    Tags
    EBFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69021-plasmid-map-sequence-id-127473

    Enlarge

  21. Linked Free ClLIS1 (M)

    Plasmid
    #182487
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Insert
    FPPS(M)-LS
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182487-plasmid-map-sequence-id-360431

    Enlarge

  23. Cer(1-158)-RGS7

    Plasmid
    #55760
    Purpose
    An amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    mCer(1-158)-RGS7 (RGS7 Aequorea victoria, Human)
    Tags
    Cer(1-158) was fused to the amino terminus of RGS…
    Expression
    Mammalian
    Mutation
    RGS7 was amplified via PCR, which added an N-term…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55760-plasmid-map-sequence-id-115314

    Enlarge

  25. Cx43K258stop-msfGFP

    Plasmid
    #69023
    Purpose
    Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx43 (Gja1 Rat)
    Tags
    V206K monomerized super folder GFP
    Expression
    Mammalian
    Mutation
    delete codons 258-381 and fuse in frame to monome…
    Promoter
    CMV
    Available Since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69023-plasmid-map-sequence-id-124708

    Enlarge

  26. MLM3720

    Plasmid
    #49944
    Purpose
    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a control
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x FLAG Tet1CDmut RH-3 (RHOXF2 Human)
    Use
    TALEN
    Tags
    3x FLAG
    Mutation
    catalytic domain of TET1 inactive (H1671Y and D16…
    Promoter
    EF1a
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  27. pLenti-CaMKIIa-SpyTag-C1C2-mCherry

    Plasmid
    #69833
    Purpose
    Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing SpyTag-C1C2-mCherry fusion. SpyTag can be used to monitor membrane localization of the C1C2 opsin.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do
    Insert
    SpyTag-C1C2
    Use
    Lentiviral
    Tags
    Trafficking signal and mCherry
    Expression
    Mammalian
    Mutation
    Inserted SpyTag after the signal peptide of C1C2.
    Promoter
    CamKIIa
    Available Since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    69833-plasmid-map-sequence-id-183098

    Enlarge

  28. MLM3709

    Plasmid
    #49943
    Purpose
    Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x FLAG Tet1CD RH-3 (RHOXF2 Human)
    Use
    TALEN
    Tags
    3x FLAG
    Promoter
    EF1a
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  30. JA740

    Plasmid
    #49939
    Purpose
    Expresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in EGFP for use as a control
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD GFP (TET1 Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  31. MLM3713

    Plasmid
    #49946
    Purpose
    Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x FLAG Tet1CD RH-4 (RHOXF2 Human)
    Use
    TALEN
    Tags
    3x FLAG
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. Linked Free NES

    Plasmid
    #182485
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Insert
    FPPS-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182485-plasmid-map-sequence-id-360436

    Enlarge

  33. Linked Free PcPTS

    Plasmid
    #182486
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Insert
    FPPS-PTS
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182486-plasmid-map-sequence-id-360437

    Enlarge

  34. MLM3762

    Plasmid
    #49947
    Purpose
    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a control
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x FLAG Tet1CDmut RH-4 (RHOXF2 Human)
    Use
    TALEN
    Tags
    3x FLAG
    Mutation
    catalytic domain of TET1 inactive (H1671Y and D16…
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  36. JA524

    Plasmid
    #49938
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in EGFP for use as a control
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD GFP (TET1 Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  37. MLM3727

    Plasmid
    #49961
    Purpose
    Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x Flag Tet1CD HB-5 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Promoter
    EF1a
    Available Since
    Jan. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  38. VC388

    Plasmid
    #49958
    Purpose
    Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x Flag Tet1CD HB-4 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. sfGFP-Cx43K258stop

    Plasmid
    #69025
    Purpose
    Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with sfGFP (V206-version) on the N-terminus. CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx43 (Gja1 Rat)
    Tags
    super folder GFP
    Expression
    Mammalian
    Mutation
    non-monomerized sfGFP fused in frame to N-terminu…
    Promoter
    CMV
    Available Since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69025-plasmid-map-sequence-id-127523

    Enlarge

  40. SL536

    Plasmid
    #49942
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    RHOXF2 Rhox homeobox family member 2 (RHOXF2 Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 6881 - 6920 of 24269 results