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Plasmid#186551PurposeInternalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cellsDepositorInsert10R-8His-ETA-EGFP
UseTags8xHis and EGFPExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-CenpB 1-166
Plasmid#108475Purposeexpression of GFP-CenpB 1-166DepositorInsertCenpB 1-166 AA (CENPB Human)
UseTagsEGFPExpressionMammalianMutationencodes only the DNA-binding domain of CENP-BPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-Myo 1d
Plasmid#134906PurposeExpression of rat Myo 1d (Myr 4) in mammalian cells.DepositorInsertMyo 1d (rat) with 3' UTR (Myo1d Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEXL flag Smad3
Plasmid#10920DepositorInsertSmad3 (SMAD3 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pIC292
Plasmid#51405PurposeRetroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)DepositorInsertSka2 IMAGE 4685972 (SKA2 Human)
UseRetroviralTagsEGFP, S tag, and TEV protease siteExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
A3Bi-Cas9n-UGI
Plasmid#119135PurposeBase editor made from full-length APOBEC3B with an L1 intron to prevent self-restrictionDepositorInsertAPOBEC3B L1 intron-Cas9 nickase-UGI
UseCRISPRTagsExpressionMutationL1 intron insertionPromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
hGFAP R239C
Plasmid#169476PurposeSingle p.Arg239Cys(R239C) mutations was individually inserted by site-directed mutagenesis into the wild-type hGFAPDepositorInsertEGFP-hGFAP (GFAP Human)
UseTagsEGFPExpressionMammalianMutationChanged R 239 to CPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Arp11 (1-417)
Plasmid#51398Purposeexpression of full-length mouse Arp11 in mammalian cellsDepositorInsertArp11 (Actr10 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC57-GFP-SFPQ-RT
Plasmid#97090PurposeEncodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.DepositorInsertEGFP-SFPQ-RT (SFPQ Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgNeg1
Plasmid#166998PurposeLentiviral expression of negative control sgRNA for CRISPRi knockdownDepositorInsertNegative control sgRNA 1
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP
Plasmid#32479PurposeCre-dependent expression of PSAM-GlyR (L141F) neuronal inhibitor, with IRES-EGFP markerDepositorInsertPSAML141F-GlyR-IRES-GFP
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsIRES-EGFPExpressionMutationPromoterSynapsinAvailable sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
perbB2K732M-ECFP
Plasmid#40269DepositorInserterbB2 (ERBB2 Human)
UseTagsecfpExpressionMammalianMutationK732MPromoterCMVAvailable sinceJan. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-RB 1-602
Plasmid#16006DepositorInsertRb (RB1 Human)
UseTagsEGFPExpressionMammalianMutationAmino acids 1-602PromoterAvailable sinceJune 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-SYN-GFP-BGHpA
Plasmid#190229PurposeExpresses EGFP in neuronal cellsDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFPH7
Plasmid#83554PurposeUsed to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.DepositorInsertADH2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterADH2Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGG184
Plasmid#165604PurposeReporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFPDepositorInserthEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
UseSynthetic BiologyTagsExpressionBacterialMutation'CAGCG' PAM replacing the 'CGGCG…PromoterlacAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Myo9b R1695M
Plasmid#134911PurposeExpression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.DepositorInsertMyo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)
UseTagsEGFPExpressionMammalianMutationchanged arginine 1695 to methioninePromoterCMV IEAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
AA015
Plasmid#216058PurposeFragmid fragment: (2A Selection) Puromycin resistance, T2A, EGFPDepositorHas ServiceCloning Grade DNAInsertP2A_v1.1; PuroR_v1.1; T2A_v1.2; EGFP_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFP97
Plasmid#83551PurposeUsed to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.DepositorInsertCYC1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterCYC1Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenLox_U6 Nlgn2
Plasmid#59358PurposeLentiviral expression vector: Neuroligin 2 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.DepositorUseLentiviralTagsExpressionMammalianMutationPromoterCAG and mouse U6Available sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
mGFAP-Halo
Plasmid#169474Purposethe host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sitesDepositorInsertGFAP (Gfap Mouse)
UseTagsHaloTagExpressionMammalianMutationChanged R 236 to HPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-LivePAR(R163A)-Hygro
Plasmid#187609PurposeEGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.DepositorInsertLivePAR(R163A)
UseLentiviralTagsEGFPExpressionMammalianMutationR163A mutation in the WWE DomainPromoterEF1AAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
L_NES 67kDa
Plasmid#201357PurposeNucleocytoplasmic transport probeDepositorInsertAdeno_NES-EGFP-6PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
M_NLS 67kDa
Plasmid#201351PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40A5-EGFP-6PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
H_NES 67kDa
Plasmid#201350PurposeNucleocytoplasmic transport probeDepositorInsertHIV_NES-EGFP-6PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
M_NES 67kDa
Plasmid#201356PurposeNucleocytoplasmic transport probeDepositorInsertMAPK_NES-EGFP-6PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Diffusive 67kDa
Plasmid#201338PurposeNucleocytoplasmic transport probeDepositorInsertEGFP-6PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Diffusive 34kDa
Plasmid#201334PurposeNucleocytoplasmic transport probeDepositorInsertEGFP-1PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK186
Plasmid#156422PurposeMammalian expression of 3a-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cellsDepositorInsertSARS-CoV-2 3a protein
UseTagsEGFP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mBAF45b IRES GFP
Plasmid#17881DepositorInsertBAF45b (Dpf1 Mouse)
UseTagsFLAG and IRES EGFPExpressionMammalianMutationPromoterAvailable sinceMay 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
GFP-GLT1a-V5
Plasmid#162517PurposeRat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loopDepositorInsertGLT1a (Slc1a2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Myo 1e
Plasmid#134905PurposeExpression of rat Myo 1e (Myr 3) in mammalian cells.DepositorInsertMyo 1e (rat) with 3' UTR (Myo1e Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mBAF45c IRES GFP
Plasmid#17882DepositorInsertBAF45c (Dpf3 Mouse)
UseTagsFLAG and IRES EGFPExpressionMammalianMutationPromoterAvailable sinceMay 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
GFP-GLT1b-V5
Plasmid#162518PurposeRat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loopDepositorInsertGLT1b (Slc1a2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-Dusp6DM_S159/197A
Plasmid#67684Purpose3' entry clone with activated Dusp6 (S159/167A)DepositorInsertdusp6 (dusp6 Zebrafish)
UseTagsExpressionMutationS159A, S197APromoterAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMG
Plasmid#138264PurposeYeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.DepositorInsertseGFP flanked by iCas9 sites
mCherry
UseCRISPRTagsExpressionYeastMutationPromoterTef1Available sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
ATF4 14: 5’ATF4.uORF1&2AUA.GFP
Plasmid#21861DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationAUG codons of uORFs1&2 were converted to AUAPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMIG II-BTLA-mGFP
Plasmid#180798PurposeExpression of mouse BTLA fused to mEGFPDepositorInsertBTLA (Btla Mouse)
UseRetroviralTagsmEGFPExpressionMutationPromoterMSCVAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pITGFP89
Plasmid#83560PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.DepositorInsertTEF1 promoter -yEGFP-CLN2PEST
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
phNFL-E396K
Plasmid#132595PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E E396K mutationDepositorInsertNEFL (NEFL Human)
UseTagsExpressionMammalianMutation1186G>A; Glu396LysPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
A3H HapII-Cas9n-UGI
Plasmid#119141PurposeBase editor made from APOBEC3H Haplotype IIDepositorInsertAPOBEC3H Haplotype II-Cas9 nickase-UGI
UseCRISPRTagsExpressionMutationNonePromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR-siKlf5
Plasmid#41740DepositorInsertKlf5 (Klf5 Mouse)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJan. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pKF-D2irTNP2AG-T2APuroR-5kwh
Plasmid#128255PurposeMammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.DepositorInserthTetR::P2A::EGFP::T2A::PuroR
UseSynthetic Biology; Flp-in expression vectorTagsExpressionMammalianMutationPromoterCMV-D2ir promoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
phNFL-P440L
Plasmid#132596PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E P440L mutationDepositorInsertNEFL (NEFL Human)
UseTagsExpressionMammalianMutation1319C>T; Pro440LeuPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
ATF4 15: 5’ATF4.uORF1stopm1.GFP
Plasmid#21862DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationStop codon for uORF1 converted to UCG (see author…PromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJK2067
Plasmid#171845PurposeC. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)DepositorInserthis-58, eGFP, mCherry
UseTagsN-terminal Tag OLLAS, V5 and C-terminal Tag PEST…ExpressionWormMutationboxB wild type and hairpin mutantPromotermex-5 promoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgNeg2
Plasmid#166999PurposeLentiviral expression of negative control sgRNA for CRISPRi knockdownDepositorInsertNegative control sgRNA 2
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP86
Plasmid#83543PurposeUsed to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.DepositorInsertRPL4A promoter-yEGFP
UseTagsExpressionYeastMutationPromoterRPL4AAvailable sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP84
Plasmid#83542PurposeUsed to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.DepositorInsertSSA1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterSSA1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only