We narrowed to 13,571 results for: GAN

1. TetO-FUW-SOX3

   Plasmid

   #61546

   Purpose

   Expresses SOX3 in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   SOX3 (SOX3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pAAV.GFAP.SV40.eGFP.SV40(polyA)

   Plasmid

   #195558

   Purpose

   Expresses eGFP under control of short GFAP promoter

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   eGFP
   

   Use

   AAV

   Tags

   No tags

   Expression

   Mammalian

   Promoter

   short GFAP

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. ZJOM143

   Plasmid

   #133667

   Purpose

   Integration of VPH1-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-VPH1-mTagBFP2 (VPH1 Budding Yeast)
   

   Tags

   mTagBFP2

   Expression

   Yeast

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57.1-Luciferase

   Plasmid

   #99283

   Purpose

   All-in-one lentivector with tet-inducible luciferase

   Depositor

   Stephen Tapscott

   Article

   Shadle et al PLoS Genet. 2017 Mar 8;13(3):e1006658. d

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMKO.1 puro GFP shRNA

   Plasmid

   #10675

   Purpose

   Negative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.

   Depositor

   William Hahn

   Article

   Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

   Insert

   GFP shRNA
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCG150_double_phiC31_attB

   Plasmid

   #169693

   Purpose

   cargo vector for the phiC31 system with, unc-119 rescue fragment, no fluorescent marker. Gateway R3R4 sites (for gene of interest insertion)

   Depositor

   Shih-Peng Chan

   Article

   Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB_iMYOD1_P2A_BAF60C2_T2A_mCh_Puro

   Plasmid

   #168807

   Purpose

   PiggyBac transposon construct with doxycycline inducible MYOD1, BAF60C + mCherry expression

   Depositor

   Prashant Mali

   Article

   Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

   Insert

   MYOD1 (MYOD1 Human, Synthetic)
   

   Use

   Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CSII-IDR2-rhesusTRIM5-FOS

   Plasmid

   #79059

   Purpose

   Express rhesus TRIM5 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   rhesusTRIM5-FOS
   

   Tags

   OneSTrEP-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. HSF1-GFPN3

   Plasmid

   #32538

   Depositor

   Stuart Calderwood

   Article

   Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

   Insert

   HSF1 (HSF1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   see depositor comments below

   Promoter

   CMV

   Available Since

   Oct. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV5 mSynCAM3

    Plasmid

    #115498

    Purpose

    expression of mouse full-length SynCAM 3

    Depositor

    Thomas Biederer

    Article

    Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

    Insert

    Cadm3 (Cadm3 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV BARK p2A mCherry

    Plasmid

    #117689

    Purpose

    CMV-iBARK-p2A-mCherry: Mammalian expression vector for Gaq silencing

    Depositor

    Baljit Khakh

    Article

    Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

    Insert

    BARKrgs peptide
    

    Tags

    HA / FLAG

    Expression

    Mammalian

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. ZJOM68

    Plasmid

    #133657

    Purpose

    Integration of TGL3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast lipid droplet.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-TGL3-mCherry (TGL3 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac1-OSF-TRIM5alpha(rhesus)

    Plasmid

    #79033

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(rhesus)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. RA-Sec61β

    Plasmid

    #153978

    Purpose

    Dimerization dependent fluorescent protein RA domain fused to ER protein Sec61β

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    Sec61 Transolocon Subunit Beta (SEC61B Human)
    

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac1-Kozak-rhTRIM5alpha-PFOS

    Plasmid

    #79038

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    rhesus TRIM5alha-PFOS
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCI_AFF-1-FLAG_H2B-RFP

    Plasmid

    #240506

    Purpose

    Cell fusion assays

    Depositor

    Benjamin Podbilewicz

    Article

    Moi et al Nat Commun. 2022 Jul 6;13(1):3880. doi:

    Insert

    AFF-1
    

    Tags

    H2B-RFP

    Expression

    Mammalian

    Available Since

    July 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21-pelB-GST-2

    Plasmid

    #228984

    Purpose

    For bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-2
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-GST-1

    Plasmid

    #228983

    Purpose

    For bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-1
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. LPC-flag-REST-FS

    Plasmid

    #41904

    Depositor

    Stephen Elledge

    Article

    Westbrook et al Nature. 2008 Mar 20;452(7185):370-4. doi

    Insert

    RE1-silencing transcription factor (REST Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    FS mutant

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NES

    Plasmid

    #124703

    Purpose

    Expresses MS2_adRNA targeting the RAB7A locus along with MCP_ADAR1 DD (E1008Q)_NES

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    MCP_ADAR2 DD (E1008Q)_NES
    

    Use

    AAV

    Tags

    NES

    Expression

    Mammalian

    Mutation

    E1008Q hyperactive mutant

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. ZJOM119

    Plasmid

    #133661

    Purpose

    Integration of NAB2-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-NAB2-mTagBFP2 (NAB2 Budding Yeast)
    

    Tags

    mTagBFP2

    Expression

    Yeast

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3-sapphireCKAR

    Plasmid

    #118468

    Purpose

    cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireCKAR
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-ExRai-AktAR

    Plasmid

    #118462

    Purpose

    cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AktAR
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti-EF1a-Ngn3ER-PuroR-mCherry

    Plasmid

    #197385

    Purpose

    To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)

    Depositor

    Qiao Zhou

    Article

    Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

    Insert

    Ngn3ER-2A-PuroR-2A-mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. BFP-Dcp1a

    Plasmid

    #153973

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1a

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA decapping enzyme 1a (DCP1A Human)
    

    Tags

    mTagBFP-c1

    Expression

    Mammalian

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pMKO.1 puro hTERT shRNA

    Plasmid

    #10688

    Depositor

    William Hahn

    Article

    Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

    Insert

    hTERT shRNA (TERT Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-ER-AKAR4

    Plasmid

    #64733

    Purpose

    Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    AKAR4
    

    Tags

    Cerulean, N-terminal 27 amino acids from CYP450, …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2

    Plasmid

    #87694

    Purpose

    Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    ERT2-PhoCl-Cre-PhoCl-ERT2
    

    Expression

    Mammalian

    Promoter

    Chicken beta actin

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. JDW 1043 (pLenti6.2_CMV_V5_LEF1_P2A_H2A_mCherry)

    Plasmid

    #233538

    Purpose

    A CMV driven human LEF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    LEF1 (LEF1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-RAB-AKARev

    Plasmid

    #118478

    Purpose

    ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-AKARev
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GFP-LiMETER-v2

    Plasmid

    #113934

    Purpose

    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

    Depositor

    Yubin Zhou

    Article

    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

    Insert

    LOV2 and Stim1 polybasic domain (PB)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDIRECT_25F

    Plasmid

    #91143

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. ZJOM149

    Plasmid

    #133646

    Purpose

    Integration of ELO3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-ELO3-mCherry (ELO3 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUASTattB-dALFANb(5mut)-mGreenLantern

    Plasmid

    #219661

    Purpose

    Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLantern

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Destabilised ALFA nanobody (5 mutations) fused to mGreenLantern
    

    Expression

    Insect

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pME-loxP-mTagBFP2-stop-pA-loxP

    Plasmid

    #75158

    Purpose

    Floxed TagBFP2 (blue) fluorophore with stop codon for inducible lines.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    loxP-mTagBFP2-stop-pA-loxP
    

    Use

    Middle entry vector for multisite gateway three f…

    Promoter

    n/a

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET22b_PstS_2

    Plasmid

    #78199

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,A197C)
    

    Expression

    Bacterial

    Mutation

    A17C; A197C (please see depositor comment below)

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SUPV3L1-HA WT

    Plasmid

    #232348

    Purpose

    Expresses human SUPV3L1/SUV3 in mammalian cells with HA tag

    Depositor

    Samantha Lewis

    Article

    Begeman et al bioRxiv [Preprint]. 2024 Aug 17:2024.08.

    Insert

    SUPV3L1 (SUPV3L1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAC-85b

    Plasmid

    #53282

    Purpose

    Contains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthase

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, crtY, crtI
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET21-Flavidin

    Plasmid

    #89881

    Purpose

    Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling

    Depositor

    Mark Howarth

    Article

    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

    Insert

    Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
    

    Tags

    6 glutamate tag (C terminal on insert)

    Expression

    Bacterial

    Mutation

    K80R, K121R, K132R, K134R

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. linker-mCitrine-PIF6-NAT

    Plasmid

    #51576

    Purpose

    Tag protein of interest with PIF6 in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PIF6 (PIL2 Mustard Weed)
    

    Tags

    linker and mCitrine

    Expression

    Yeast

    Mutation

    1-100aa of PIF6

    Promoter

    none

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge