We narrowed to 8,780 results for: construct
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Plasmid#50969PurposeAAV2 transfer vector containing the InSynC (miniSOG-VAMP2-citrine) constructDepositorInsertminiSOG-VAMP2-citrine (Vamp2 Mouse, Arabdopsis thaliana)
UseAAVTagscitrine and miniSOGPromoterhuman synapsin promoterAvailable SinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Sik-WT
Plasmid#210722PurposeConstruct for stable expression of mouse wild type Ptk6 (Sik) in mammalian cells.DepositorInsertProtein tyrosine kinase 6 (Ptk6 Mouse)
ExpressionMammalianAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-TetR
Plasmid#103813PurposeBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No3
Plasmid#194898PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-3 targeting the promoter of human MECP2DepositorInsertsgRNA targeting human MECP2 promoter No.3
UseLentiviralExpressionMammalianPromoterU6Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No9
Plasmid#194903PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-9 targeting the promoter of human MECP2.DepositorInsertsgRNA targeting human MECP2 promoter No.9
UseLentiviralExpressionMammalianPromoterU6Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-CYFIP2.GFP
Plasmid#109140PurposeGFP-tagged CYFIP2 expression constructDepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro_SV40polyA
Plasmid#124546PurposeCloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertWT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro
ExpressionMammalianMutationWT, WTPromoterMinimal ADEAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2 F2bbs kan GCANNNAGGk
Plasmid#18752DepositorInsert3 tandem zinc fingers
ExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…Available SinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOPS0384
Plasmid#133234PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSYN-VWF-039
Plasmid#120292PurposeVWF expression construct expressing D'D3 domains with C1099A/C1142A mutations in D3 domain as an Fc fusion proteinDepositorInsertVWF D1-D2-D'-D3-Fc
TagsFcExpressionMammalianMutationC1099A/C1142APromoterCMVAvailable SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1.1
Plasmid#32467DepositorInsertG-GECO1.1
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…Available SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOPS0385
Plasmid#133235PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), gusA (pOGG083) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0386
Plasmid#133236PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), celB (pOGG050) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0383
Plasmid#133233PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), sfGFP (pOGG037) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0380
Plasmid#133230PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL2-MCT2Prom0.6
Plasmid#87353PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 0.6 kbpDepositorInsertMCT2 (SLC16A7 Human)
UseLuciferaseExpressionBacterial and MammalianPromoter0.6 kbp proximal promoter of human MCT2 (nested d…Available SinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-KD-mAbl
Plasmid#69028PurposeRetorviral vector encoding Kinase Defective mouse c-AblDepositorInsertmouse c-Abl Type VI - kinase defective
UseRetroviralTagsFLAG tag inserted at Internal SalI site (2934 bp)ExpressionMammalianMutationThe KD (kinase defective) mutation is at Lysine 2…Available SinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
DNMT3b KO Hyg
Plasmid#16650DepositorInsertDNMT3b knock-out construct (DNMT3B Human)
ExpressionBacterialAvailable SinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj10a-IRES-EGFP
Plasmid#164947PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj10a and EGFPDepositorInsertkcnj10a (kcnj10a Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPPromoteractinb (actb2) promoterAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
ath5:H2B-RFP
Plasmid#105960Purposetransgenesis, appears rather late, likely due to long RFP maturation time but is brighter than most other Ath5 constructDepositorAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgULK1-1
Plasmid#109004PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No4
Plasmid#194899PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-4 targeting the promoter of human MECP2DepositorInsertsgRNA targeting human MECP2 promoter No.4
UseLentiviralExpressionMammalianPromoterU6Available SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330_Tuba1b sgRNA / hSpCas9
Plasmid#172835PurposeMammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP1054
Plasmid#119241Purposeconstruction intermediateDepositorTypeEmpty backboneExpressionBacterialAvailable SinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMP1050
Plasmid#119240Purposeconstruction intermediateDepositorTypeEmpty backboneExpressionBacterialAvailable SinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1 _701-902
Plasmid#86821Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion constructDepositorAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL2-1
Plasmid#109008PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C-2xFLAG
Plasmid#130979PurposeExpression of KIF1C-2xFLAG in mammalian cells.DepositorInsertKIF1C-2xFLAG (KIF1C Human)
Tags2xFLAGExpressionMammalianMutation4 silent mutations that make this construct resis…PromoterCMVAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMU103
Plasmid#73934PurposeThis is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.DepositorInsertbar and GUS cassettes
ExpressionPlantPromoter35SAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1 _462-699
Plasmid#86819Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion constructDepositorAvailable SinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1 _568-637
Plasmid#86820Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion constructDepositorAvailable SinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)
Plasmid#61939PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coliDepositorInsertsTags6x HISExpressionBacterialMutationConstruct ends at residue 156PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (925-953)
Plasmid#73744PurposeExpression of GFP-PP-RPN2 (925-953) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
Tags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 932-953 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA
Plasmid#124545PurposeCloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdelta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
ExpressionMammalianMutationd11-41, d11-41PromoterMinimal ADEAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]
Plasmid#159800PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
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t2. pT7-7-SNCA-5Lys
Plasmid#107294PurposePlasmid for the expression of the construct SNCA-5Lys. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Lys and 7 GlyDepositorAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
r. pT7-7-SNCA-5Asn
Plasmid#107295PurposePlasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asn and 7 GlyDepositorAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
s2. pT7-7-SNCA-5Asp
Plasmid#107293PurposePlasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asp and 7 GlyDepositorAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (918-932)
Plasmid#73748PurposeExpression of GFP-PP-RPN2 (918-932) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
Tags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-932 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only