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p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]

Plasmid

#71805

Purpose

Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5 Myc-RIP1

Plasmid

#44159

Depositor

Xin Lin

Article

Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

Insert

RIP1 (RIPK1 Human)

Tags

Myc

Expression

Mammalian

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pPCK

Plasmid

#133128

Purpose

a recombinant plasmid, expressing pckA PEP-carboxykinase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

PEP-carboxykinase gene

Expression

Bacterial

Promoter

PEP-carboxykinase promoter

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_h7SK

Plasmid

#102842

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_h7SKpromoter

Use

As pcr template

Promoter

h7SK

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEBTet_NADP-Snifit-nucl

Plasmid

#113918

Purpose

Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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acta1-mRuby2-dGBP

Plasmid

#163855

Purpose

Zebrafish muscle specific expression of mRuby2-dVHH[GFP]

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

dVHH[GFP]

Use

In vitro transcription of rna

Tags

Ruby2

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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BB2_AB

Plasmid

#98534

Purpose

empty BB2 for transcription unit cloning

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMC3-yeGFP

Plasmid

#176181

Purpose

yeast MoClo level-0 part plasmid type 3 containing yeast-enhanced GFP

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

yEGFP

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FKBP-Venus-173-C

Plasmid

#87617

Purpose

FKBP-tagged C-terminal Venus (YFP) fragment for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

FKBP-Venus173-C

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-CAR-GECO1

Plasmid

#45493

Purpose

Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

Depositor

Robert Campbell

Article

Wu et al ACS Chem Neurosci. 2013 Mar 19.

Insert

CAR-GECO1

Expression

Mammalian

Mutation

R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

Promoter

CMV

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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SB_loop

Plasmid

#110103

Purpose

pBXPHM3 with scaffold Sybody of the loop library

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Insert

scaffold Sybody of the loop library

Tags

His

Expression

Bacterial

Promoter

PBAD

Available Since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

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pKM3

Plasmid

#154168

Purpose

Empty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Type

Empty backbone

Expression

Insect

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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p{CFD4-EYFP-3xP3::DsRed}

Plasmid

#86863

Purpose

Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Type

Empty backbone

Expression

Insect

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_Hygro

Plasmid

#167859

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-miSFIT-2x

Plasmid

#124691

Purpose

Tuning expression of EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

EGFP

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pENO1-dTom-NATr

Plasmid

#52208

Purpose

Constitutive expression of Candida codon-optimized dTomato with NAT selectable marker

Depositor

Robert Wheeler

Article

Gratacap et al Dis Model Mech. 2013 Sep;6(5):1260-70. d

Insert

dTomato

Expression

Bacterial and Yeast

Mutation

codon optimized for Candida species

Promoter

ENO1

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pBbdCas9S_Psyn-sgRNA500

Plasmid

#149640

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_Blasto

Plasmid

#167856

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_TBA1A

Plasmid

#111695

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

TBA1A (TUBA1A Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJMP8835

Plasmid

#220902

Purpose

MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PG-dCas9-novo

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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