176,436 results
-
-
pCMV-MMLVgag-3xNES-ABE8e-NG
Plasmid#181754PurposeMMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorInsertMMLVgag-3xNES-ABE8e-NG
TagsFlagExpressionMammalianPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-DJ-N589X
Plasmid#130878PurposeEncodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codonDepositorInsertAAV-DJ cap N589X
UseAAVExpressionMammalianMutationN589XPromoterUnknownAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-32a(+)-TGFB3
Plasmid#120288PurposeProduce human recombinant TGFB3 in DE3 cellsDepositorInsertTransforming Growth Factor-β3 (TGFB3 Synthetic, Human)
Tags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Flag-c-Myc
Plasmid#102626PurposeExpresses c-Myc in mammalian cellsDepositorInsertMYC proto-oncogene, bHLH transcription factor (MYC Human)
UseLentiviralTagsFlagExpressionMammalianPromoterCMVAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG mito-GCaMP5G
Plasmid#105009Purposemitochondrial calcium sensorDepositorInsertGCaMP5G
ExpressionMammalianPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRGEN-CMV-CjCas9
Plasmid#89752PurposeExpression of CjCas9 in mammalian cellsDepositorInsertCas9
UseCRISPRTagsSV40-HAExpressionMammalianAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TRE-KRAB-dCas9-IRES-BFP
Plasmid#85449PurposeLentiviral vector expressing a KRAB-dCas9 fusion protein and BFPDepositorPublicationInsertKRAB-dCas9-IRES-BFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianPromoterTRE3GAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TAC-ERP2
Plasmid#80478PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonExpressionMammalianAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-dCas9-5xPlat2AflD
Plasmid#82560PurposeExpression vector for Cas9 peptide array (linker length: 22aa).DepositorInsertdCas9-5xPlat2AflD
ExpressionMammalianAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJL-TRBO
Plasmid#80082PurposeAn improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMAL-c2X
Plasmid#75286PurposeExpresses proteins in the cytoplasm as fusions to maltose-binding proteinDepositorTypeEmpty backboneTagsmaltose-binding proteinExpressionBacterialPromotertacAvailable sinceJuly 18, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCRISPRyl
Plasmid#70007PurposeCRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertionDepositorPublicationInsertsCodon optimized Cas9 from S. pyogenes
sgRNA expression cassette
UseCRISPR and Synthetic BiologyExpressionYeastPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p11-LacY-wtx1
Plasmid#69056PurposeThe reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.DepositorAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Str-Ii_VSVG-SBP-EGFP
Plasmid#65300Purposesynchronize trafficking of VSVG from the ER (RUSH system)DepositorInsertVSV-G
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAVS1-TLR targeting vector
Plasmid#64215PurposeTargeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) constructDepositorInserttraffic light reporter (TLR) (AAVS1 Human)
UseHuman targetingMutationsee publication for detailsPromoterCAGAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMF230
Plasmid#62546PurposeBroad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.DepositorInsertGFP mut2
UseBroad host range plasmidPromotertrcAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQFlag-USP7 WT puroR
Plasmid#46751PurposeRetroviral vector that expresses wild type Flag-tagged human USP7DepositorAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GA-NES
Plasmid#61018PurposeddGFP A-NESDepositorInsertddGFP A
TagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-TOMM20-N-10
Plasmid#55328PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456DepositorAvailable sinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by
