12,323 results

1. MXS_Kaede

   Plasmid

   #62413

   Purpose

   MXS_chaining vector with Kaede

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   Kaede
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. hgRNA-D21_pLKO-Hyg

   Plasmid

   #100562

   Purpose

   hgRNA-D21 in a lentiviral backbone

   Depositor

   George Church

   Article

   Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

   Insert

   hgRNA-D21
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBTK614

   Plasmid

   #110608

   Purpose

   BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS

   Plasmid

   #121835

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL2_164

   Plasmid

   #199096

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Nili Ostrov, Charlie Gilbert, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pIP501 ORI plus NGS barcode 4, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL2_165

   Plasmid

   #199097

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Nili Ostrov, Charlie Gilbert, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pSC101ts ORI plus NGS barcode 10, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL2_161

   Plasmid

   #199093

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Nili Ostrov, Charlie Gilbert, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pAL5000 ORI plus NGS barcode 1, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL2_162

   Plasmid

   #199094

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Nili Ostrov, Charlie Gilbert, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pUB110 ORI plus NGS barcode 2, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

   Plasmid

   #121841

   Purpose

   pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AV11_pDonor.EP.S1

    Plasmid

    #100296

    Purpose

    AAVS1-targeting expression plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    PuroR.T2A.EGFP expression unit
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDOC-K-glmS-lacIZ

    Plasmid

    #158061

    Purpose

    Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    lacIZ operon
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L

    Plasmid

    #121833

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7)/Dnmt3a-3L (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTW102

    Plasmid

    #115945

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 DDd-L7Ae
    

    Use

    Synthetic Biology

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9CR4-VQR

    Plasmid

    #120224

    Purpose

    pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015

    Depositor

    Christopher Reisch

    Insert

    SpCas9-VQR
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Mutation

    Mutations D1135V/R1335V/T1337R

    Promoter

    pTet

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFH57

    Plasmid

    #125883

    Purpose

    Level0 Soybean GmUBI Pol II promoter

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    Level0 Soybean GmUbi Pol II promoter
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4

    Plasmid

    #123658

    Purpose

    DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control without sgRNA following mU6 promoter.

    Depositor

    Stanley Qi

    Article

    Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. FB009

    Plasmid

    #119707

    Purpose

    Transcriptional Unit (TU) for geneticin (G418) resistance in fungi obtained by combining FB001+FB005+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    PtrpC::nptII::Ttub
    

    Use

    Synthetic Biology

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBVR5

    Plasmid

    #167511

    Purpose

    Broad-Host range expression vector, pBBR origin, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    VanRAM-mRFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PVanCC

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAS007

    Plasmid

    #164868

    Purpose

    This plasmid can be used to study DNA looping in vivo. pAS007 contains the phiKO2 cB operator sequences but can be modified to insert any DNA operators of interest.

    Depositor

    Keith Shearwin

    Article

    Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612

    Insert

    phiKO2 operators
    

    Use

    Synthetic Biology

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDUET-aaCPRct-A13AMO

    Plasmid

    #20116

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    p450 reductase and Amorphadiene oxidase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only