We narrowed to 11,439 results for: 112

1. pLB(N)CX-FLAG-CH3

   Plasmid

   #133718

   Purpose

   Retroviral expression vector driving CH3 domain fragment of human p300 (EP300), with N-terminal FLAG tag.

   Depositor

   James A. DeCaprio

   Article

   Borger et al J Virol. 2006 May . 80(9):4292-303.

   Insert

   EP300 (EP300 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mutation

   partial (not complete) p300 fragment, CH3 domain …

   Promoter

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a - Ec-ClpX D144L

   Plasmid

   #111514

   Purpose

   Expresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

   Insert

   ClpX
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Aspartatic acid 144 to Leucine

   Promoter

   T7

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRevLCav2FeS

   Plasmid

   #60808

   Purpose

   to disrupt iron sulfur cluster in yeast Rev3

   Depositor

   Tahir Tahirov, Youri Pavlov

   Article

   Baranovskiy et al J Biol Chem. 2012 May 18;287(21):17281-7

   Insert

   REV3 (REV3 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   contains 647 C-terminal amino acids and 335 bp of…

   Promoter

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

4. PRR4-bio-His

   Plasmid

   #52075

   Purpose

   Expresses full-length Proline-rich protein 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PRR4 (PRR4 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQTEV-C6orf60

   Plasmid

   #34813

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

   Insert

   C6orf60 (FAM184A Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. human CRKL transgenic construct

   Plasmid

   #38186

   Depositor

   Nora Heisterkamp

   Article

   Hemmeryckx et al Cancer Res. 2001 Feb 15;61(4):1398-405.

   Insert

   CRKL (CRKL Human)
   

   Use

   Mouse transgenic

   Tags

   Expression

   Mutation

   Contains human CRKL introns

   Promoter

   human CRKL

   Available since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

7. HOXB6-FLAGA-pSP65

   Plasmid

   #20994

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res. 1996 Mar 1;24(5):898-

   Insert

   HOXB6 (HOXB6 Human)
   

   Use

   In vitro trans/trans

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pET30-GBF-E6

   Plasmid

   #21696

   Depositor

   James Baleja

   Article

   Liu et al J Mol Biol. 2009 Mar 6. 386(4):1123-37.

   Insert

   protein G domain B1 fused human papillomavirus type 16 amno acid residue 2-142 (E6 Human papillomavirus type 16)
   

   Use

   Tags

   Protein G B1 domain

   Expression

   Bacterial

   Mutation

   GB1 (1-56aa) fusion tag with mutations at three p…

   Promoter

   Available since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pCAG-FLAG-puro-PTPIP51(1-470)

   Plasmid

   #170536

   Purpose

   Expresses PTPIP51(1-470) in human HeLa cells after transfection

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pBpaRS3tRNA

    Plasmid

    #197102

    Purpose

    To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

    Insert

    TyrRS variant, amber suppressor tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-3FLAG-EEA1

    Plasmid

    #176491

    Purpose

    For lentiviral transduction of 3FLAG-EEA1

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pS-sg:GFP

    Plasmid

    #196296

    Purpose

    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSs

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. ppk301-T2A-QF2 HDR plasmid

    Plasmid

    #130667

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
    

    Use

    Donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pM-ABE

    Plasmid

    #196292

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding ABE in place of viral GP
    

    Use

    CRISPR

    Tags

    3×flag

    Expression

    Plant

    Mutation

    See depositor comments below

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLNHA-C1-Hs4E-BP3

    Plasmid

    #79439

    Purpose

    Expresses lambdaN-HA-tagged human 4E-BP3 in Mammalian cells

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    Hs4E-BP3 (EIF4EBP3 Human)
    

    Use

    Tags

    lambdaN-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pM-Cas9

    Plasmid

    #196325

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding SpCas9 in place of viral GP
    

    Use

    CRISPR

    Tags

    3×flag

    Expression

    Plant

    Mutation

    See depositor comments below

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF2601

    Plasmid

    #144133

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    POU6F2 (POU6F2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pM-Cas12a

    Plasmid

    #196291

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding LbCas12a in place of viral GP (cas12a Synthetic)
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Plant

    Mutation

    See depositor comments below

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pS-cr:GFP

    Plasmid

    #196295

    Purpose

    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
    

    Use

    CRISPR

    Tags

    SpeI-DR-BsaI-DR-SpeI

    Expression

    Plant

    Mutation

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1_T6B -mCherry

    Plasmid

    #205600

    Purpose

    expresses human T6B peptide fused with mChery in mammalian cells

    Depositor

    Marc Friedlander

    Article

    Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

    Insert

    T6B peptide (fragment of human TRNC6B 599-683aa) (TNRC6B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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