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pBabe-puro-mRipk3

Plasmid

#78830

Purpose

retroviral expression of mRIPK3

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (Ripk3 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pLX304 3xFlag-eRF1_IRES-GFP

Plasmid

#198393

Purpose

eRF1 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

eRF1 (ETF1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 proHB-EGF NC

Plasmid

#11605

Depositor

Rosalyn Adam

Article

Kim et al Cancer Res. 2005 Sep 15. 65(18):8242-9.

Insert

HB-EGF (HBEGF Human)

Expression

Mammalian

Mutation

Non-cleavable HB-EGF: Pro149 to Thr160 replaced w…

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pEGFP-n1-AICD

Plasmid

#69925

Purpose

mammalian expression of human AICD fused to EGFP

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Insert

AICD (APP Human)

Tags

EGFP

Expression

Mammalian

Mutation

47 aa long

Promoter

CMV

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MD2-HA

Plasmid

#133065

Purpose

Retroviral expression of mouse MD2 tagged with HA

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

MD2 (Ly96 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-GST-eIF4E K119A

Plasmid

#112818

Purpose

Expresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Eif4e (Eif4e Mouse)

Tags

GST

Expression

Bacterial

Mutation

K119 mutated to A, increasing affinity for cap st…

Promoter

T7

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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LUC01 (L/Sec/P)

Plasmid

#112144

Purpose

Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

Depositor

Paul Copeland

Article

Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

Insert

Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)

Expression

Mammalian

Mutation

Cysteine 258 mutated to selenocysteine (U)

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pQCX-CD28 WT

Plasmid

#223640

Purpose

Retroviral expression of mouse CD28 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (Cd28 Mouse)

Use

Retroviral; mammalian expression

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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PX459-TP53-exon5

Plasmid

#217456

Purpose

For TP53 knockout targeting exon 5 of TP53

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CRISPR Cas9 guide for TP53 (TP53 Human)

Use

CRISPR

Expression

Mammalian

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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ETS1_pet28a

Plasmid

#131663

Purpose

Bacterial expression of ETS gene ETS1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETS1 (ETS1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_FH-AGO2-WT

Plasmid

#92006

Purpose

Expresses FLAG-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

FLAG-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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shMEK1/2 v2 puro

Plasmid

#72568

Purpose

Hairpin targeting MEK1 and MEK2 (murine).

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

MEK1/2 (Map2k1, Map2k2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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PD2529 human alpha5 full

Plasmid

#221395

Purpose

Mammalian expression of human integrin alpha5 full length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin alpha5 full length (ITGA5 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA)

Expression

Mammalian

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pETM33_EIF4E_eiF4E

Plasmid

#178474

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

EIF4E_eiF4E (EIF4E Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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CDK4-3XFlag-pBabe

Plasmid

#73558

Purpose

vector encoding a fusion protein between CDK4 and 3 Flag tags

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pmirGLO-3'UTR mouse Il6

Plasmid

#207126

Purpose

Luciferase vector containing 3'UTR for mouse Il6

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Interleukin 6 (Il6) (Il6 Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

PGK promoter

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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p1.2-Hyg-Fur

Plasmid

#162774

Purpose

Soluble human PACE/furin protease expression

Depositor

Ivan Vorobiev

Article

Kovnir et al Acta Naturae. 2018 Jan-Mar;10(1):51-65.

Insert

Human PACE/furin protease soluble (FURIN Human)

Expression

Mammalian

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-GATA2

Plasmid

#131602

Purpose

expression of human GATA2 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

GATA2 (GATA2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EGFR D837A

Plasmid

#11014

Purpose

Retroviral construct for expressing human EGFR with D837A mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D837A (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

D837A

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pYFP-ErbB2

Plasmid

#66948

Purpose

Mammalian expression of rat ErbB2 tagged with YFP

Depositor

Mads Lerdrup, Bo van_Deurs

Article

Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

Insert

ErbB2 (Erbb2 Rat)

Tags

6xHis, V5 Tag, and YFP

Expression

Mammalian

Mutation

(See depositor comment below on mutations A24T an…

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only