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TOMM20-mTurquoise-Cdc42-G12V-DeltaCaaX

Plasmid

#176121

Purpose

Constitutivley active, mitochondrial localized Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

CDC42 (CDC42 Human)

Expression

Mammalian

Mutation

G12V

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ14901

Plasmid

#239276

Purpose

Expresses gG1 for pertubing endogenous human GAPDH mRNA via CRISPR-TO

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

PspCas13b-hGAPDH-gRNA1 (GAPDH Human)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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MAC_N_MED13

Plasmid

#111689

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

MED13 (MED13 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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EGFR D837A

Plasmid

#11014

Purpose

Retroviral construct for expressing human EGFR with D837A mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D837A (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

D837A

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2b-WT

Plasmid

#45698

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pCAX APPs-695-alpha

Plasmid

#30147

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein - soluble (APP Human)

Expression

Mammalian

Mutation

amino acids 1–612

Available Since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/CALR wt-FLAG

Plasmid

#204532

Purpose

Mammalian expression of human CALR wt-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR wt-FLAG (CALR Human)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-Calr-KDEL-IRES-Puromycin

Plasmid

#134664

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

calreticulin (CALR Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 18-413

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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Pb-TRE3G-TYR-HygR

Plasmid

#195508

Purpose

inducible Piggybac vector expressing human TYR CDS with hygromycin selection

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

TYR (TYR Human)

Use

Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK5

Plasmid

#53166

Purpose

Expresses GST-HA tagged human MEK5 from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Tags

GST and HA

Expression

Bacterial

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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BK alpha subunit

Plasmid

#113566

Purpose

mouse BK channel alpha subunit expression vector

Depositor

Robert Brenner

Article

Gruslova et al J Gen Physiol. 2012 Jan;139(1):57-67. do

Insert

Kcnma1 (Kcnma1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pmIL-6promoterEGFP

Plasmid

#112896

Purpose

To monitor the activation of the murine IL-6 promoter by eGFP expression

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

murine IL-6 promoter (Il6 Mouse)

Expression

Mammalian

Promoter

IL-6 promoter

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLPCX-Cx43-IRES-GFP

Plasmid

#65433

Purpose

Mammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral production

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

Cx43 (GJA1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ADAR2DD_E488Q_Ago2

Plasmid

#205599

Purpose

expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) fused with human Ago2 in mammalian cells

Depositor

Marc Friedlander

Article

Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

Insert

hAgo2 (AGO2 Human)

Tags

ADAR2DD_E488Q (hyperactive mutant)

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-GFP11-Actin

Plasmid

#181966

Purpose

Human beta actin tagged with GFP11.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

GFP11-Actin (ACTB Human)

Tags

GFP11

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pmirGLO-3'UTR mouse Il6

Plasmid

#207126

Purpose

Luciferase vector containing 3'UTR for mouse Il6

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Interleukin 6 (Il6) (Il6 Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

PGK promoter

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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DsRed-rab7 DN

Plasmid

#12662

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

DsRed and HA

Expression

Mammalian

Mutation

T22N

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO APEX2-eIF4E1

Plasmid

#129644

Purpose

Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

EIF4E1 (EIF4E Human)

Tags

APEX2

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFudioFRTPSD-95TealW

Plasmid

#134299

Purpose

Expresses PSD95 fused to Teal fluorescent protein upon coexpression with the recombinase flippase (Flp)

Depositor

Elly Nedivi

Article

Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

Insert

PSD-95-Teal (Dlg4 Mouse, Rat)

Use

Lentiviral; Flp/frt

Tags

Teal

Expression

Mammalian

Promoter

UbC

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH_TNF-SBP-EGFP

Plasmid

#65282

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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