We narrowed to 66,069 results for: des.1

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Flag-Jun4A-Myc

Plasmid

#47444

Purpose

mammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)

Depositor

Axel Behrens

Article

Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

Insert

Jun 4A mutant (Jun Mouse)

Tags

Flag and myc

Expression

Mammalian

Mutation

four N-terminal residues phosphorylated by JNK, s…

Promoter

CMV

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pX462-hPRKAA1-gRNA_B

Plasmid

#74375

Purpose

gRNA_B to knockout human AMPK alpha 1 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)

Use

CRISPR

Promoter

U6

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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GAG-CRErec

Plasmid

#119971

Purpose

Expresses GAG (FMLV) fused with CRE recombinase for the production of VLPs loaded with CRE protein

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Insert

GAG-nlsCRErec

Tags

nls

Expression

Mammalian

Promoter

hCMV

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRGECO1b.WPRE.SV40

Plasmid

#100857

Purpose

AAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRGECO1b

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA7

Plasmid

#26683

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 7 (KPNA7 Human)

Tags

T7

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag Yap1 Y357F

Plasmid

#18882

Depositor

Yosef Shaul

Article

Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

Insert

Yap1 Y357F (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Y357F

Available Since

Aug. 14, 2008

Availability

Academic Institutions and Nonprofits only

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pMK347 (BSD-P2A-mAID)

Plasmid

#121181

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

BSD-P2A-mAID

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgGAL4-1

Plasmid

#46915

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgGAL4-1
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV and U6

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-ESYN-R5

Plasmid

#32581

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

Enhanced Synapsin Promoter

Use

Gateway Cloning

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3_Sec61B-V5-APEX2

Plasmid

#83411

Purpose

Expressed C-term tagged APEX2 on Sec61B in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

Insert

Sec61B-V5-APEX2 (SEC61B Synthetic, Human)

Tags

APEX2 and V5

Expression

Mammalian

Promoter

CMV-F

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-Flex-TCB

Plasmid

#48332

Purpose

Cre-dependant TVA-mCherry fusion

Depositor

Liqun Luo

Article

Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

Insert

TVA receptor mCherry

Use

AAV

Tags

mCherry fusion to TVA receptor

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

Plasmid

#176270

Purpose

Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

TadA-TadA*(8e)-dCasMINI-V4

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA5

Plasmid

#26681

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 5 (KPNA5 Human)

Tags

T7 (MASMTGGQQMG)

Expression

Mammalian

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-B

Plasmid

#104448

Purpose

Expression of human VAP-B fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein B (VAPB Human)

Tags

EGFP and HA

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pKC-4.06

Plasmid

#112084

Purpose

NMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)

Depositor

James Inglese

Article

Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1

Insert

3XFLAG firefly luciferase-beta-globin 39PTC (HBB Mouse, Photinus pyralis, Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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p45sheLL

Plasmid

#37323

Depositor

John Schiller

Article

Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

Insert

HPV45L1+L2

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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T7-TPase

Plasmid

#51818

Purpose

For in vitro transcription of Tol2 transposase using T7 Promoter

Depositor

Elly Tanaka

Article

Khattak et al Nat Protoc. 2014 Mar;9(3):529-40. doi: 1

Insert

Tol2 Transposase

Use

Unspecified

Promoter

T7

Available Since

April 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-tTA

Plasmid

#104102

Purpose

Expresses tTA2 under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tTA2

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC2-10XUAS-IVS-mCD8::GFP

Plasmid

#26214

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

mCD8::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-HaloTag7-mGFP

Plasmid

#184903

Purpose

Stable expression of HaloTag7-mGFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

monomeric EGFP

Tags

HaloTag7

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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