4,179 results

1. YAP1 (S6A) - V5 in pLX304

   Plasmid

   #42562

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   S61A, S109A, S127A, S128A, S131A, S163A, S164A, S…

   Promoter

   CMV

   Available Since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSECC

   Plasmid

   #60820

   Purpose

   3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre

   Depositor

   Tyler Jacks

   Article

   Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature

   Inserts

   Cas9
   Cre
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   BsmBI site eliminated by C->A; E308G

   Promoter

   EFS and EFS (after Cas9-2A)

   Available Since

   Nov. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmEGFP_a_tubulin_IRES_puro2b

   Plasmid

   #21042

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   alpha Tubulin (TUBA1B Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-eNpHR 3.0-EYFP

   Plasmid

   #26972

   Purpose

   AAV expression of eNpHR 3.0 fused to EYFP driven by humanized Synapsin promoter for optogenetic inhibition

   Depositor

   Karl Deisseroth

   Has Service

   AAV2 and AAV5

   Insert

   eNpHR 3.0
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Trafficking Signal (TS) ER Export Signal

   Promoter

   hSyn

   Available Since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. hIRE1a wt

   Plasmid

   #20744

   Depositor

   Fumihiko Urano

   Article

   Lipson et al PLoS ONE. 2008 . 3(2):e1648.

   Insert

   IRE1 (ERN1 Human)
   

   Use

   Lentiviral; Destination vector

   Expression

   Mammalian

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE TRE dCas9

   Plasmid

   #50915

   Purpose

   Tet-regulatable dCas9 lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe puro Myr HA PIK3CA

   Plasmid

   #12523

   Depositor

   Jean Zhao

   Article

   Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

   Insert

   PIK3CA Myr (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Myristoylation. Constitutively active allele; I14…

   Available Since

   Aug. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLOX-CWBmi1

   Plasmid

   #12240

   Depositor

   Didier Trono

   Article

   Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

   Insert

   Bmi1 (Bmi1 Mouse)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Mutation

   CMV promoter upstream of mBmi-1. LoxP in 3' …

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. MyrPalm-CKAR

   Plasmid

   #14862

   Depositor

   Alexandra Newton

   Article

   Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

   Insert

   C kinase activity reporter
   

   Tags

   CFP, Myr/Palm, and YFP

   Expression

   Mammalian

   Available Since

   Aug. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HA-PHLPP2

    Plasmid

    #22403

    Depositor

    Alexandra Newton

    Article

    Brognard et al Mol Cell. 2007 Mar 23. 25(6):917-31.

    Insert

    PHLPP2 (PHLPP2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. HDM-SARS2-Spike-delta21

    Plasmid

    #155130

    Purpose

    Encodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotyping

    Depositor

    Jesse Bloom

    Article

    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

    Insert

    SARS-CoV-2 Spike-delta21 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Codon optimized to H. sapiens using IDT codon opt…

    Promoter

    CMV

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti CMV/TO RasV12 Puro (w119-1)

    Plasmid

    #22262

    Depositor

    Eric Campeau

    Insert

    HRAS G12V (HRAS Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    G12V mutant

    Available Since

    Dec. 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC0048-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q)

    Plasmid

    #103864

    Purpose

    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GSGGGGS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-Rubicon

    Plasmid

    #21636

    Depositor

    Tamotsu Yoshimori

    Article

    Matsunaga et al Nat Cell Biol. 2009 Apr . 11(4):385-96.

    Insert

    Rubicon (1700021K19Rik Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    *(see below)

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLM-mCerulean-cMyc

    Plasmid

    #23244

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCerulean-2A-MYC (MYC Human, synthetic fluorescent protein)
    

    Use

    Lentiviral

    Available Since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV4-p100

    Plasmid

    #23287

    Depositor

    Warner Greene

    Article

    Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

    Insert

    NFkB p100 subunit (NFKB2 Human)
    

    Expression

    Mammalian

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSYFP2-C1

    Plasmid

    #22878

    Depositor

    Dorus Gadella

    Article

    Kremers et al Biochemistry. 2006 May 30. 45(21):6570-8

    Insert

    SYFP2
    

    Expression

    Mammalian

    Available Since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJL-SaKKH-BE3

    Plasmid

    #85170

    Purpose

    Expresses SaKKH-BE3 in mammalian cells [Edit]

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    SaKKH-BE3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEos4b-C1

    Plasmid

    #54812

    Purpose

    Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson, Loren Looger

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Type

    Empty backbone

    Tags

    mEos4b

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. PKC delta WT

    Plasmid

    #16386

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC delta isoform 2 (Prkcd Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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