We narrowed to 10,761 results for: T7
-
Plasmid#110829PurposeBacterial expression of E.coli DHFR N23PP/S148A mutantDepositorInsertecDHFR
ExpressionBacterialMutationN23 mutated to P23; a proline residue inserted be…PromoterT7Available SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-At_PRORP3-His
Plasmid#67871Purposebacterial expression of PRORP3 (At4g21900), full coding sequence + C-term. His tagDepositorAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT5R1AtR2NtB2
Plasmid#160888PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T5
ExpressionBacterialPromoterrbcS-T5: T7, chaperones: T11Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT1R1AtR2NtB2
Plasmid#160885PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T1
ExpressionBacterialPromoterrbcS-T1: T7, chaperones: T8Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXB13 (human) HIS-tag pET
Plasmid#8543DepositorInsertHOXB13 (HOXB13 Human)
TagsHis and T7ExpressionBacterialMutationmakes protein by Western and by transcription/tra…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree _KA2_ZIKV NS5
Plasmid#145368PurposeCell-free/mammalian expression vector for Zika virus NS5 protein with N-term mCherryDepositorInsertZIKV NS5
Tags8xHis and mCherryExpressionBacterialPromoterT7- CMV/CAGAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
SLIRP.HMG6.wt.fl
Plasmid#49821Purposeexpression vector for MBP-SLIRP fusionDepositorInsertSLIRP SRA stem-loop interacting RNA binding protein (SLIRP Human)
TagsE. coli Maltose Binding ProteinExpressionBacterialMutationwildtype codon-optimized for E. coli expressionPromoterT7Available SinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSKB3-SaGGR-I206F-L377H (JBEI-7202)
Plasmid#110152PurposepSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-I206F-L377H mutant geneDepositorInsertGGR
TagsTEV-cleavable His-tagExpressionBacterialMutationSaGGR-I206F-L377H mutant genePromoterT7Available SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDB368
Plasmid#110276PurposegHH_44 with a N-terminal 10-His DsbC TEV-peptide fusionDepositorInsertgHH_44
Tags10-His, Tev recognition peptide, and mature DsbC …ExpressionBacterialPromoterT7Available SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-Y100F
Plasmid#110826PurposeBacterial expression of E.coli DHFR Y100F mutantDepositorInsertecDHFR
ExpressionBacterialMutationY100 mutated to F100PromoterT7Available SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAL950
Plasmid#119748Purposeprecursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to CysteineDepositorInsertprecusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
TagsnoneExpressionBacterialMutationInternal NdeI site removed and mutations Alanine …PromoterT7Available SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-PKAsub-RLuc8N-NES
Plasmid#138211PurposeEncodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertmCherry-PKAsub-RLuc8N-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GTF2A1
Plasmid#67076PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
-
Cenp-B DBD INCENP TSS/AAA mCherry
Plasmid#45234PurposeTarget INCENP (with TSS mutated to AAA) to centromeres via CENP-B DNA binding domain (DBD)DepositorInsertCenp-B DBD, INCENP-ΔCEN with AAA mutation, mCherry
TagsCENP-B DBD, DNA binding domain of CENP-B and mChe…ExpressionMammalianMutationINCENP C-terminal TSS mutated to AAAPromotert7Available SinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1T1
Plasmid#97327PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1T1
Tags6xHis - TEVExpressionBacterialMutationAddition of T residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1T2
Plasmid#97328PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1T2
Tags6xHis - TEVExpressionBacterialMutationAddition of two T residues after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-his-3C_Clu3B6p
Plasmid#100093Purposeexpresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptideDepositorInsert14-3-3 sigma human (SFN Human)
TagsHSPB6 peptide and His6ExpressionBacterialMutation75-77 residues changed to AAA to facilitate cryst…PromoterT7Available SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
drd4a_L (TAL 3006)
Plasmid#35998DepositorInsertTAL array targeting drd4a (drd4a Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianAvailable SinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
6his R.3-AR GUSpET28a+/pLysS
Plasmid#61157PurposeN-terminal His tagged round 3 aldehyde-resistant GUS expression vectorDepositorInsertGUSAR
Tags6x HisExpressionBacterialMutationN66D, D151N, A219V, I396T, T480A, Q498R, D508E an…PromoterT7Available SinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (64-502, construct 13i, C77A, MG-31-28)
Plasmid#110747PurposeBacterial expression of human USP30 (construct 13i)DepositorInsertUSP30 (USP30 Human)
TagsN-His6-GST-3CExpressionBacterialMutationC77A, F348D, M350S, I353E + insertion deletion: 6…PromoterT7Available SinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKV005
Plasmid#64763PurposeRecombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable Myc tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassettePromoternoneAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 BTF3
Plasmid#67059PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
pBTK305
Plasmid#110594PurposeBTK Type 4 terminator for golden gate assemblyDepositorInsertT7 terminator
UseSynthetic BiologyAvailable SinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCellFree_G03 SMAD2
Plasmid#67095PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLz74_Rubisco_CsoS2_Npeptide_fusion
Plasmid#140840PurposeSame as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallizationDepositorInsertForm IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS
TagsCsoS2 N-peptide consensus to CbbL C-terminus and …ExpressionBacterialPromoterT7Available SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
B. thetaiotaomicron mRNA toehold switch sensor
Plasmid#110707PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertB. thetaiotaomicron species specific toehold switch sensor
UseSynthetic BiologyPromoterT7Available SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-eSpCas9
Plasmid#126769PurposeExpression of increased fidelity eSpCas9 in bacterial cellsDepositorInserteSpCas9
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationK848A, K1003A, R1060APromoterT7Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-rbN1 S77A
Plasmid#32945DepositorInsertSLc9a3r1 (SLC9A3R1 rabbit)
TagsT7 tag, his, and partial FLAGExpressionMammalianMutationChange Serine77 to AlaninePromoterCMVAvailable SinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
kank4_pJet1.2
Plasmid#121982Purposefor generating kank4 riboprobes for WISH in zebrafishDepositorAvailable SinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TYK2
Plasmid#67133PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
-
-
-