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Plasmid#127824PurposeProduction of recombinant human ribosomal protein S15DepositorInsertRPS15 (RPS15 Human)
UseTagsHis6 - Thioredoxin - TEVExpressionBacterialMutationT136DPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_M42FDHFR_WTTS
Plasmid#91237Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationM42FPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_M42FG121VDHFR_R166QTS
Plasmid#91238Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationM42FG121 and R166QPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_M42FG121VDHFR_WTTS
Plasmid#91239Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationM42FG121VPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_W22HDHFR_R166QTS
Plasmid#91240Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationR166Q and W22HPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_WTEcoliDHFR_R166QTS
Plasmid#91242Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationR166QTSPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_D27NDHFR_R166QTS
Plasmid#91243Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationD27N and R166QPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31VDHFR_R166QTS
Plasmid#91245Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationF31V and R166QPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31YG121VDHFR_R166QTS
Plasmid#91228Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationF31YG121 and R166QPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31YL54IDHFR_R166QTS
Plasmid#91230Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationF31YL54I and R166QPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31YL54IDHFR_WTTS
Plasmid#91231Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationF31YL54IPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_G121VDHFR_R166QTS
Plasmid#91232Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationG121V and R166QPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_L54IDHFR_R166QTS
Plasmid#91234Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationL54I and R166QPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_L54IDHFR_WTTS
Plasmid#91235Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationL54IPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_M42FDHFR_R166QTS
Plasmid#91236Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationM42F and R166QPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-Cdc45 (delta 154–164)
Plasmid#126879PurposeExpresses crystallisation construct of human Cdc45 in bacteriaDepositorInsertHuman Cdc45 (CDC45 Human)
UseTagsNo tagExpressionBacterialMutationdeletion amino acid 154-164PromoterT7 promoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
B. adolescentis mRNA toehold switch sensor
Plasmid#110710PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertB. adolescentis species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-YJR129c
Plasmid#85118Purposebacterial expression of YJR129cDepositorInsertEFM3 (EFM3 Budding Yeast)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/68
Plasmid#113921PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68DepositorInsertMBP-NanoLucCP-65/68
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/66
Plasmid#113920PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66DepositorInsertMBP-NanoLucCP-65/66
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDB367
Plasmid#110277PurposegHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusionDepositorInsertgHEEE_02
UseTags10-His, Tev recognition peptide, and mature DsbC …ExpressionBacterialMutationPromoterT7Available sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
F. prausnitzii mRNA toehold switch sensor
Plasmid#110716PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertF. prausnitzii species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
C. difficile mRNA toehold switch sensor
Plasmid#110714PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertC. difficile species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
R. hominis mRNA species specific sensor
Plasmid#110713PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertR. hominis species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
E. rectale mRNA toehold switch sensor
Plasmid#110712PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertE. rectale species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
B. breve mRNA toehold switch sensor
Plasmid#110711PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertB. breve species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
B. longum mRNA toehold switch sensor
Plasmid#110709PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertB. longum species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
E. coli mRNA toehold switch sensor
Plasmid#110708PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertE. coli species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
F. prausnitzii 16S toehold switch sensor
Plasmid#110705PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertF. prausnitzii 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
R. hominis 16S toehold switch sensor
Plasmid#110703PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertR. hominis 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
E. rectale 16S toehold switch sensor
Plasmid#110702PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertE. rectale 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
B. breve 16S toehold switch sensor
Plasmid#110701PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertB. breve 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
B. adolescentis 16S toehold switch sensor
Plasmid#110700PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertB. adolescentis 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
B. longum 16S toehold switch sensor
Plasmid#110699PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertB. longum 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
B. thetaiotaomicron 16S toehold switch sensor
Plasmid#110697PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertB. thetaiotaomicron 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
T7T3-PacI_MafB_ISH
Plasmid#87670PurposeIn situ probe for mouse MafbDepositorInsertMafb (Mafb Mouse)
UseTagsExpressionBacterialMutationPromoterT7/T3Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin (1-76; F4A)
Plasmid#110757PurposeBacterial expression of Ubiquitin (1-76; F4A)DepositorInsertUBB (UBB Human)
UseTagsExpressionBacterialMutationF4APromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKB3-SaGGR-F219L (JBEI-7186)
Plasmid#110151PurposepSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant geneDepositorInsertGGR
UseTagsTEV-cleavable His-tagExpressionBacterialMutationSaGGR-F219L mutant genePromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys
Plasmid#110541PurposeBacterial protein of E.coli DHFR C85A/C152S mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationC85 mutated to A85, C152 mutated to S152PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET27b-ecDHFR-N23PP
Plasmid#110828PurposeBacterial expression of E.coli DHFR N23PP mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationN23 mutated to P23; a proline residue inserted be…PromoterT7Available sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only