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Plasmid#177667PurposeSIBR Intron 4 (Int4) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only
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pYPQ134-tRNA2.0
Plasmid#158396PurposeGolden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-recAmu-sacB
Plasmid#158717PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.DepositorInsertrecA
UseTagsExpressionBacterialMutationR61CPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
P823_eSpCas9(1.1)-RecA
Plasmid#87264PurposeHuman codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiencyDepositorInsertRecA
UseTagsFLAGExpressionMammalianMutationPromoterCBhAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
LTR5HS S. pyogenes scaffold CARGO array
Plasmid#191316PurposeContains an array of guide RNAs targeting LTR5Hs elementsDepositorInsertArray of guide RNAs
UseCRISPRTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135-tRNA2.0
Plasmid#158397PurposeGolden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Zeo
Plasmid#227258PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Zeo cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJCC_039 FnCas12a
Plasmid#171666PurposeFor bacterial expression of FnCas12a with a C-terminal sorting motif and His tagDepositorInsertFnCas12a
UseTags6X His and sortase A sorting motifExpressionBacterialMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM808
Plasmid#134181PurposeLentiviral sgRNA plasmid with blasticidin resistanceDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuidPuro-hTP73_sgRNA-1
Plasmid#88855PurposeCRISPR KO of Trp73DepositorInsertTrp73
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro Non-targeting sgRNA 3
Plasmid#109003PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertNon-targeting sgRNA #3
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136-tRNA2.0
Plasmid#158398PurposeGolden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTC223
Plasmid#70019PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7DepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSIBR003
Plasmid#177666PurposeSIBR Intron 3 (Int3) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-Cas9C-VPR
Plasmid#80933PurposeExpresses Cas9C-VPR in mammalian cells.DepositorInsertCas9C-VPR
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEPSA5Cas9syn
Plasmid#206965PurposeS. aureus genome editing vector removed these two motifs AGGNNNNNGAT and CCAYNNNNNNTGTDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
Plasmid#222992PurposeMammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNADepositorInsertHuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
UseCRISPRTagsPuroExpressionMammalianMutationPromoterpCAGAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Puro
Plasmid#167911PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC017 - LwCas13a guide expression backbone with tRNAval promoter
Plasmid#91907PurposeBackbone for cloning LwCas13a guides under tRNAval promoterDepositorInsertLwCas13a Guide site
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#112924PurposepiggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant markerDepositorInsertEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
UsePiggybac transposon vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U6-(BbsI)sgRNA_CAG-Venus-bpA
Plasmid#86985PurposeFor cloning and expression of sgRNA together with Venus expressionDepositorInsertVenus
UseTagsExpressionMammalianMutationVenus GFP variantPromoterCAGAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGE1108
Plasmid#202772PurposeCRISPR-KO in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC103-PBNeo-U6-sgRNA-Expression
Plasmid#48228PurposesgRNA expression on PiggyBac vector with Neo-selectable markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
p1195-pssAAV.U1a.hNme2Cas9
Plasmid#129534PurposeAAV vector expressing Nme2Cas9DepositorInserthuman codon-optimized Nme2Cas9
UseAAVTags2xNLS and NLS-3xHA-NLSExpressionMutationPromoterU1aAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12i1
Plasmid#120882PurposeExpresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.DepositorInsertCas12i1
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#83946PurposeCas9-carrying vector with kanMX markerDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x2
Plasmid#58772PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEPSA5Cas9syn2
Plasmid#206963PurposeS. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGATDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-GCN4
Plasmid#78899PurposeExpresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell cultureDepositorInsertdCas9-10XGCN4
UseCRISPRTagsHAExpressionInsectMutationPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
eGFP L202 gRNA
Plasmid#119132PurposegRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.DepositorInserteGFP L202 gRNA
UseCRISPRTagsExpressionMutationNonePromoterU6Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM441
Plasmid#61271PurposeAlso referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTC217
Plasmid#70018PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1bDepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
DHFR-PP7-VP64_T2A_GFP
Plasmid#86167PurposeExpresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cellsDepositorInsertDHFR-PP7-VP64_T2A_GFP
UseCRISPR and LentiviralTagsDestabilized domain (N-terminus version) of E.col…ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ220 (AsCpf1)
Plasmid#86208PurposeAsCpf1 Gateway entry plasmidDepositorInsertAsCpf1
UseGateway compatible cpf1 vectorsTags5' and 3' NLSExpressionPlantMutationPromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
nontarget S. pyogenes scaffold CARGO array
Plasmid#191319PurposeContains an array of nontargeting guide RNAsDepositorInsertArray of guide RNAs
UseCRISPRTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCB2-sgRNA
Plasmid#129463PurposeTemplate plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).DepositorInsertpgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpyC-GFP
Plasmid#220191PurposesgRNA expression vector - pBG42 promoter with GFP in sgRNA siteDepositorInsertpBG42 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG42Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSGKP_AcsgRNA
Plasmid#203807PurposepSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.DepositorInsertAcgRNA
UseCRISPRTagsExpressionMutationPromoterJ23119Available sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-3XHA
Plasmid#127881PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA taggingDepositorInsertGFP-Puro-3XHA (PPP2CA Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAcCAST-sgRNA_entry (CJT83)
Plasmid#181785PurposeExpresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
UseTagsExpressionBacterialMutationPromoterLac and J23119Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC020 - LseCas13a from Listeria seeligeri
Plasmid#91910PurposeExpresses LseCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLseCas13a and guide expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUb RxCas13d + RxCas13d guide RNA
Plasmid#176303PurposeExpresses RxCas13d and its associated guide RNA in Drosophila cellsDepositorInsertRxCas13d
UseTagsHA tagExpressionInsectMutationPromoterUbi-p63eAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUb dPspCas13b + PspCas13b guide RNA
Plasmid#176306PurposeExpresses catalytic dead PspCas13b and its associated guide RNA in Drosophila cellsDepositorInsertPspCas13b
UseTagsHA tagExpressionInsectMutationH133A, H1058APromoterUbi-p63eAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
eGFP L138 gRNA
Plasmid#119133PurposegRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.DepositorInserteGFP L138 gRNA
UseCRISPRTagsExpressionMutationNonePromoterU6Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b
Plasmid#184565PurposeInducible expression of PbuCas13b nuclease in bacteria.DepositorInsertCas13b nuclease from Prevotella buccae
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShoCAST-sgRNA_entry (BO1)
Plasmid#181786PurposeExpresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
UseTagsExpressionBacterialMutationPromoterLac and J23119Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
CL7-LbCas12a
Plasmid#164658PurposeBacterial expression of LbCas12a using CL7/Im7DepositorInsertLbCas12a
UseTagsThioredoxin, CL7, SUMOExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9
Plasmid#78903PurposeExpresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cellsDepositorInsertdCas9
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJIT163-2NLSCas9
Plasmid#53064PurposeExpression of rice codon-optimized Cas9 in plant cellsDepositorInsertCas9
UsePlant expressionTagsExpressionMutationRice codon optimizedPromoter2x35SAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PpU6P-sgRNA-L5L2
Plasmid#113738PurposeGateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR; Gateway entry vectorTagsExpressionMutationPromoterP. patens U6 promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only