We narrowed to 4,337 results for: U6 gRNA

1. pSIL-eGFP-shACTN4

   Plasmid

   #52679

   Purpose

   shRNA against α-actinin-4 with eGFP transfection marker

   Depositor

   Johannes Hell

   Article

   Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

   Insert

   ACTN4 (Actn4 Rat)
   

   Use

   RNAi

   Tags

   CMV-EGFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-RFP-IKZF3-sh2

   Plasmid

   #69043

   Purpose

   3rd generation lentiviral vector expressing IKZF3 shRNA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF3 (IKZF3 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJZC25

   Plasmid

   #62328

   Purpose

   sgRNA + 1x MS2 with MCP-VP64 effector for mammalian cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA + 1x MS2 binding module
   MCP-VP64
   

   Use

   Lentiviral

   Tags

   VP64

   Expression

   Mammalian

   Mutation

   Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

   Promoter

   CMV and U6

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. PLL5.0-Anillin ShRNA-GFP-AnillinFL

   Plasmid

   #187272

   Purpose

   Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   U6, CMV

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL5.0 mCherry-hCortKD-Flcortmut3YD

   Plasmid

   #187266

   Purpose

   Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

   Depositor

   Alpha Yap

   Article

   Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

   Insert

   Cortactin (CTTN Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   3YD (Y412, Y470, Y486)

   Promoter

   U6, 5'LTR

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLL5.0 hCortKD-Flcortmut3YF mCherry

   Plasmid

   #187265

   Purpose

   Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

   Depositor

   Alpha Yap

   Article

   Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

   Insert

   Cortactin (CTTN Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   3YF (Y412, Y470, Y486)

   Promoter

   U6, 5'LTR

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSIL-eGFP-shACTN2

   Plasmid

   #52677

   Purpose

   shRNA against α-actinin-2 with eGFP transfection marker

   Depositor

   Johannes Hell

   Article

   Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

   Insert

   ACTN2 (Actn2 Rat)
   

   Use

   RNAi

   Tags

   CMV-EGFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJZC32

   Plasmid

   #62327

   Purpose

   sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA
   MCP-VP64
   

   Use

   Lentiviral

   Tags

   VP64

   Expression

   Mammalian

   Mutation

   Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

   Promoter

   CMV and U6

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. eSpCas9(1.1)_No_FLAG_ATP1A1_G2

   Plasmid

   #86610

   Purpose

   Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via nhej using ouabain

   Tags

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTE4938

    Plasmid

    #107527

    Purpose

    Expresses Fn crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Fn crRNA
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and human U6

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

    Plasmid

    #86611

    Purpose

    Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Tags

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-shGBP1.1.mKO2

    Plasmid

    #85209

    Purpose

    TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    GBP1 (guanylate binding protein 1) (GBP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSicoR-sh4EBP2

    Plasmid

    #81121

    Purpose

    expresses an shRNA targeting murine 4E-BP2

    Depositor

    Angelique Bordey

    Article

    Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

    Insert

    4E-BP2 shRNA (Eif4ebp2 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSicoR-Ef1a-mCh-Puro-GFPi

    Plasmid

    #31848

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    GFP RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMKO.1-shCcnb1

    Plasmid

    #162793

    Purpose

    Ccnb1 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Ccnb1 shRNA (Ccnb1 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJZC41

    Plasmid

    #62332

    Purpose

    sgRNA (no RNA aptamer addition) with PCP-VP64 effector for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA
    PCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMKO.1-shBirc5

    Plasmid

    #160949

    Purpose

    Birc5 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Birc5 shRNA (Birc5 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSicoR-Ef1a-mCh-GFPi

    Plasmid

    #31849

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    GFP RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSicoR-Ef1a-mCh-Puro-Luci

    Plasmid

    #31850

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    Luciferase RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLL5.0 hCortKD-Flcortmut3YF EGFP

    Plasmid

    #187263

    Purpose

    Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    3YF (Y412, Y470, Y486)

    Promoter

    U6, 5'LTR

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge