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mCh-Mfn2

Plasmid

#141156

Purpose

Mammalian expression and labeling mitofusin 2

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Mitofusin 2 (MFN2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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LV-TRE-VP64 human MyoD-T2A-dsRedExpress2

Plasmid

#60629

Purpose

Expresses VP64 human MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP64 human MyoD (MYOD1 Human)

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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PERK 2: PERK.K618A.9E10.pCDNA

Plasmid

#21815

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (K618A) (Eif2ak3 Mouse)

Tags

Myc (9E10)

Expression

Mammalian

Mutation

kinase dead K618A mutant

Available Since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc3-CAND1

Plasmid

#19948

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)

Tags

myc3

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
M7 pdnPKA-GFP

Plasmid

#16716

Depositor

Randall Moon

Article

Ungar et al Dev Biol. 1996 Aug 25;178(1):186-91. doi

Insert

dnPKA (Prkar1a Mouse)

Use

Xenopus expression

Tags

GFP

Mutation

Dominant negative. Mutations at cAMP binding site…

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc CDC4 R465C

Plasmid

#16653

Depositor

Bert Vogelstein

Article

Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

Insert

CDC4 R465C (FBXW7 Human)

Tags

myc

Expression

Mammalian

Mutation

R465C

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pmGFP-Sec16L

Plasmid

#15776

Depositor

Benjamin Glick

Article

Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

Insert

Sec16L (SEC16A Human)

Tags

mGFP

Expression

Mammalian

Mutation

K466R

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pGL3-KRAS

Plasmid

#13919

Depositor

Frank Slack

Article

Johnson et al Cell. 2005 Mar 11. 120(5):635-47.

Insert

KRAS 3'UTR (KRAS Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pDS275

Plasmid

#34972

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

T406A, T407A, I532A

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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6-His-MBP-TEV-FnCpf1

Plasmid

#90094

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 (humanized)

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCMVβ-p300.DY-myc

Plasmid

#30490

Depositor

Tso-Pang Yao

Insert

p300 (EP300 Human)

Tags

Myc

Expression

Mammalian

Mutation

aspartic acid 1399 converted to tyrosine (Acetyla…

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pBB572

Plasmid

#27397

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

ibpA, ibpB (ibpB, ibpA E.coli)

Expression

Bacterial

Available Since

March 28, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO mouse shRNA 1 rictor

Plasmid

#21341

Depositor

David Sabatini

Article

Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

Insert

rictor shRNA 1 (Rictor Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only

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pGL2Basic-EcadK1/EpaIMUT/EboxMUT/Ebox2MUT

Plasmid

#19291

Depositor

Eric Fearon

Article

Hajra et al Cancer Res. 2002 Mar 15. 62(6):1613-8.

Insert

E-cadherin

Use

Luciferase

Expression

Mammalian

Mutation

E-cad promoter sequences from -108 to +125 Ebo…

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pBABE puro mouse IRS-2 myc

Plasmid

#11373

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-2 (Irs2 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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Cas9-2A-Cre

Plasmid

#68468

Purpose

co-expressing Cas9 and Cre recombinase

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

Cas9 and Cre recombinase

Use

CRISPR

Tags

P2A-Cre-Myc

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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HA Nprl3 pRK5

Plasmid

#46330

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Nprl3 (NPRL3 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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Ub-G76V-YFP

Plasmid

#11949

Depositor

Nico Dantuma

Article

Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

Insert

Ub-G76V-YFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of YFP. Glycine 76 …

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pF145 pAcGFP1 SOD1G93A

Plasmid

#26406

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

G93A

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Arf1 ActQ71L

Plasmid

#10832

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf1 Q71L (ARF1 Human)

Tags

HA

Expression

Mammalian

Mutation

Q71L. Defective in GTP hydrolysis. Constitutive…

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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