159,371 results

1. pET21a-cys-His6-OtUBD

   Plasmid

   #190091

   Purpose

   Expresses OtUBD with a non-cleavable N-terminal 6xHis tag as well as an engineered N-terminal cysteine in E. coli

   Depositor

   Mark Hochstrasser

   Article

   Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

   Insert

   OtUBD
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-2xFYVE

   Plasmid

   #140050

   Purpose

   Expresses mCherry-tagged HRS 2xFYVE in mammalian cells

   Depositor

   Harald Stenmark

   Article

   Sneeggen et al Nat Commun. 2019 Jun 28;10(1):2850. doi:

   Insert

   tandem HRS FYVE domain (Hgs Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   two FYVE domains (amino acids 147-223), linked by…

   Promoter

   CMV

   Available since

   May 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHCMV-AmphoEnv

   Plasmid

   #15799

   Depositor

   Miguel Sena-Esteves

   Article

   Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

   Insert

   4070A Amphotropic Murine leukemia virus envelope gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

   Plasmid

   #185919

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-hSyn-RFP

   Plasmid

   #22909

   Depositor

   Edward Callaway

   Article

   Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

   Has Service

   Cloning Grade DNA

   Insert

   human synapsin I promoter driving DsRedExpress (SYN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCB-1

   Plasmid

   #210387

   Purpose

   All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

   Depositor

   Gilles van Wezel

   Article

   Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

   Inserts

   dCas9
   CymR
   sgRNA cassette
   

   Use

   CRISPR; Integrative vector for use in streptomyces

   Tags

   Expression

   Mutation

   BsaI-flanked RFP cassette inserted in place of sp…

   Promoter

   SP11 and SP30

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEP PolI

   Plasmid

   #11722

   Depositor

   Lawrence Loeb

   Article

   Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

   Insert

   DNA polymerase I EP (polA E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D424A, I709N, A759R. Error-prone version of PolI.

   Promoter

   Available since

   Aug. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. STR-KDEL_PSAP-SBP-mCherry

   Plasmid

   #180440

   Purpose

   Co-expresses (via IRES) streptavidin-KDEL and Prosaposin-SBP-mCherry

   Depositor

   Shawn Ferguson

   Article

   Devireddy et al J Cell Biol. 2022 Feb 7;221(2):e20210404

   Insert

   Prosaposin (PSAP )
   

   Use

   Tags

   SBP-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVone-AAV9-CMV-eGFP

   Plasmid

   #230928

   Purpose

   pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.

   Depositor

   Qizhao Wang

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP/golgin-160

    Plasmid

    #180630

    Purpose

    Expression of WT golgin-160

    Depositor

    Carolyn Machamer

    Article

    Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

    Insert

    golgin-160 (GOLGA3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-mAmetrine-CAAX

    Plasmid

    #216132

    Purpose

    Lentiviral vector for expressing mAmetrine localizing to the plasma membrane.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-mAmetrine-CAAX-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p23-puro-EGFP-RPA194

    Plasmid

    #179237

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    RPA194 (POLR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLminP_Luc2P_RE38

    Plasmid

    #90378

    Purpose

    RELB - NFkB gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    MAF

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_gRNA -hMLHdn-Puro

    Plasmid

    #191105

    Purpose

    Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    hMLH1dn
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    I31 silent mutation (ATA to ATC) to remove early …

    Promoter

    EF-1a

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHSE401

    Plasmid

    #62201

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    35S and AtU6-26p

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. FRP795_insul-(lexA-box)8-PminCYC1-Citrine-TCYC1

    Plasmid

    #58435

    Purpose

    Induces Citrine expression from a promoter containing 8 lexA boxes in yeast cells

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    CitrineA206K
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    insul-(lexA-box)8-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX307-SARS-CoV-2-3CL

    Plasmid

    #160278

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. FosB

    Plasmid

    #187908

    Purpose

    Expresses FosB in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    FosB (Fosb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGFP

    Plasmid

    #32748

    Depositor

    Jeanne Hardy

    Article

    Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

    Insert

    GFP
    

    Use

    Tags

    DEVDFQGPCNDSSDPLVVAASIIGILHLILWILDRL

    Expression

    Mammalian

    Mutation

    S65T

    Promoter

    CMV

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWT PolI

    Plasmid

    #11721

    Depositor

    Lawrence Loeb

    Article

    Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

    Insert

    DNA polymerase I (polA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wild-type

    Promoter

    Available since

    Aug. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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