We narrowed to 2,905 results for: BFP

1. pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2

   Plasmid

   #169531

   Purpose

   Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W

   Plasmid

   #105034

   Purpose

   Lentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Ptpn23 (Ptpn23 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

   Plasmid

   #67983

   Purpose

   Cas9 activity reporter (control) with GFP and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB700 lenti gRNA EBFP2

   Plasmid

   #167905

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-W

   Plasmid

   #200475

   Purpose

   Lentiviral vector expressing gRNA targeting GFP

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

   Plasmid

   #105039

   Purpose

   Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Tsc2 (Tsc2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(GATA3(13))-PGKpuro2ABFP-W

   Plasmid

   #208548

   Purpose

   Lentiviral vector expressing gRNA targeting human GATA3

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GATA3(13) (GATA3 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(GATA3(12))-PGKpuro2ABFP-W

   Plasmid

   #208547

   Purpose

   Lentiviral vector expressing gRNA targeting human GATA3

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GATA3(12) (GATA3 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

   Plasmid

   #98973

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 2 and Cas9(D10A)
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

    Plasmid

    #98972

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 1 and Cas9(D10A)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPRISM-Stop-gcry-TagBFP

    Plasmid

    #117771

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    3x3 Stop sequence
    

    Use

    Synthetic Biology

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUltra-hot-H2B-mTagBFP2

    Plasmid

    #161792

    Purpose

    3rd generation lentiviral multicistronic vector for mammalian expression of mCherry, H2B-mTagBFP2, and one gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Insert

    H2B-mTagBFP2
    

    Use

    Lentiviral; Multicistronic

    Tags

    mCherry and mTagBFP2

    Expression

    Mammalian

    Mutation

    I174A in the mTagBFP2 protein according to PLoS O…

    Promoter

    UbC

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRE-HRI-V5-IRES-BFP

    Plasmid

    #202435

    Purpose

    Lentiviral vector expression of HRI and TagBFP

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    HRI (EIF2AK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-FKBP-mTagBFP2-SOScat

    Plasmid

    #178866

    Purpose

    Mammalian expression of SOS1(Rem-Cdc25) fused to FKBP-mTagBFP2

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    FKBP-mTagBFP2-SOS(Rem-Cdc25) (SOS1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS1-rab-3P::his24::tagBFP2

    Plasmid

    #124344

    Purpose

    Nuclear Localized Pan-Neuronal tagBFP2

    Depositor

    Andrew Leifer

    Insert

    rab3, tagBFP2 (rab-3 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. U6::sgBFP-U6::sgKrasG12D-pX333-red

    Plasmid

    #149448

    Purpose

    pX333 backbone expressing 2 sgRNA targeting BFP and KRAS G12D to edit mouse KRAS G12D allele.

    Depositor

    Jonathan Chernoff

    Article

    Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

    Insert

    sgRNA BFP and mouse KRAS G12D
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAS1-rab-3P::his-24::tagBFP

    Plasmid

    #124343

    Purpose

    Nuclear Localized Pan-Neuronal tagBFP

    Depositor

    Andrew Leifer

    Article

    Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

    Insert

    rab-3, tagBFP (rab-3 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. StrKDEL-IRES-mannosidase II-mTagBFP2

    Plasmid

    #165460

    Purpose

    Expressed mannII-BFP simultaneously with a RUSH Hook Str-KDEL.

    Depositor

    David Stephens

    Article

    McCaughey et al J Cell Sci. 2021 Aug 5. pii: 271201. doi

    Insert

    Partial ManII-mTag2BFP fusion (Man2a1 Mouse)
    

    Tags

    Str-KDEL and mTag2BFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W

    Plasmid

    #105040

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPRISM-Fuse-cmlc2-TagBFP

    Plasmid

    #117786

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    mRFP
    

    Use

    Synthetic Biology

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Str-KDEL_flGalT-SBP-tagBFP

    Plasmid

    #65274

    Purpose

    synchronize trafficking of GalT from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    galactosidase
    

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKLV2-U6gRNA5(SOX2_v32_7-4)-PGKpuroBFP-W

    Plasmid

    #211986

    Purpose

    Express gRNA against SOX2 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting SOX2 (SOX2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKLV2-U6gRNA5(SOX2_v32_7-5)-PGKpuroBFP-W

    Plasmid

    #211987

    Purpose

    Express gRNA against SOX2 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting SOX2 (SOX2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTS2410-Tier1-PhCMV-Myr_mTagBFP2

    Plasmid

    #169535

    Purpose

    Tier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven membrane-localized monomeric Tag blue fluorescent protein 2
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPRISM-TagRFP-cmlc2-TagBFP

    Plasmid

    #117768

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FKBP12-CB2x2-mTagBFP2-HAx2

    Plasmid

    #207146

    Purpose

    Tandem calcium-buffer with rapalog dimerizer

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    Calbindin-2 (CALB2 Human)
    

    Tags

    FKBP12, HA, and mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKLV2-U6gRNA5(BCL11B-E1(14))-PGKpuro2ABFP-W

    Plasmid

    #208554

    Purpose

    Lentiviral vector expressing gRNA targeting human BCL11B-E1

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    BCL11B-E1(14) (BCL11B Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Thy1.2-NLS_HA_Dre-P2A-BFP

    Plasmid

    #51275

    Purpose

    Co-expression of the site-specific recombinase Dre and BFP under the control of the Thy1.2 expression cassette

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    NLS_HA_Dre-P2A-BFP
    

    Promoter

    Thy1.2

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L

    Plasmid

    #52478

    Purpose

    Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T

    Depositor

    Sebastian Seung

    Article

    Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

    Inserts

    EGFP
    nuclear mTagBFP
    Synaptophysin-TagRFP-T
    

    Use

    Rabies virus

    Tags

    TagRFP-T

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKLV2-U6gRNA5(Nprl2-g1)-PGKpuroBFP-W

    Plasmid

    #105037

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nprl2 (Nprl2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pKLV2-U6gRNA5(Nprl2-g2)-PGKpuroBFP-W

    Plasmid

    #105038

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nprl2 (Nprl2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. dTomato_LD 0_CS G_tTA-BFP

    Plasmid

    #58871

    Purpose

    MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBS-TRE-mTagBFP2-WPRE

    Plasmid

    #193332

    Purpose

    Expresses mTagBFP2 under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    mTagBFP2
    

    Expression

    Mammalian

    Mutation

    A XhoI site in the coding sequence is silenced

    Promoter

    TRE

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Str-KDEL_ManII-SBP-tagBFP

    Plasmid

    #65254

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mCherry_LD 0_CS G_tTA-BFP

    Plasmid

    #58856

    Purpose

    MESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAC-U63-tgRNA-nlsBFP

    Plasmid

    #169029

    Purpose

    gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

    Depositor

    Chun Han

    Article

    Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pKLV2-U6gRNA5(Nelfb-g1)-PGKpuroBFP-W

    Plasmid

    #105023

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfb (Cobra1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKLV2-U6gRNA5(Nelfb-g2)-PGKpuroBFP-W

    Plasmid

    #105024

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfb (Cobra1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-W

    Plasmid

    #105025

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfcd (Th1l Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pKLV2-U6gRNA5(Nelfcd-g2)-PGKpuroBFP-W

    Plasmid

    #105026

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfcd (Th1l Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge