We narrowed to 5,039 results for: Apoe

1. pCAGEN-dPspCas13b-NES-mCherry

   Plasmid

   #233609

   Purpose

   Expression of catalytically-inactive PspCas13b in mammalian cells

   Depositor

   Shintaro Iwasaki

   Article

   Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

   Insert

   dPspCas13b-NES-mCherry
   

   Use

   CRISPR

   Tags

   3xFlag and mCherry

   Expression

   Mutation

   H133A/H1058A

   Promoter

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAGEN-dPspCas13b-4EHP-NES-mCherry

   Plasmid

   #233610

   Purpose

   Expression of catalytically-inactive PspCas13b fused with 4EHP in mammalian cells

   Depositor

   Shintaro Iwasaki

   Article

   Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

   Insert

   dPspCas13b-4EHP-NES-mCherry
   

   Use

   CRISPR

   Tags

   3xFlag and mCherry

   Expression

   Mutation

   H133A/H1058A

   Promoter

   Available since

   March 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRJPaph-bjGFP

   Plasmid

   #67032

   Purpose

   Plasmid for Paph-bjGFP integration downstream of B. diazoefficiens (B. japonicum) scoI

   Depositor

   Julia Vorholt

   Article

   Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

   Inserts

   B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
   bjGFP
   

   Use

   Conjugative bacterial vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRJPaph-mChe

   Plasmid

   #67034

   Purpose

   Plasmid for Paph-mCherry integration downstream of B. diazoefficiens (B. japonicum) scoI

   Depositor

   Julia Vorholt

   Article

   Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

   Inserts

   B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
   mCherry
   

   Use

   Conjugative bacterial vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRJPaph-sYFP2

   Plasmid

   #67033

   Purpose

   Plasmid for Paph-sYFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI

   Depositor

   Julia Vorholt

   Article

   Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

   Inserts

   B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
   sYFP2
   

   Use

   Conjugative bacterial vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHAGE2 mCherry-Atl3

   Plasmid

   #86680

   Purpose

   Lentivirus to stably express fluorescent protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   ATL3 (ATL3 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-AQP9

   Plasmid

   #48808

   Purpose

   Express human AQP9, which induces filopodia formation

   Depositor

   Kenneth Jacobson, Vesa Loitto

   Article

   Loitto et al Exp Cell Res. 2007 Apr 15;313(7):1295-30

   Insert

   AQP9 (AQP9 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3 Barr2 flag

   Plasmid

   #14685

   Depositor

   Robert Lefkowitz

   Article

   Laporte et al Proc Natl Acad Sci U S A. 1999 Mar 30. 9

   Insert

   beta-arrestin 2 (Arrb2 Rat)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2007

   Availability

   Academic Institutions and Nonprofits only

9. p1A

   Plasmid

   #162692

   Purpose

   Expresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   H. neapolitanus CbbLS and S. elongatus Prk
   

   Use

   Tags

   N terminal 6x His tag on PRK

   Expression

   Bacterial

   Mutation

   Promoter

   PLtet0-1 promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBH-UAS-secA5-YFP

    Plasmid

    #32359

    Purpose

    Live imaging of apoptotic cells in zebrafish

    Depositor

    Randall Peterson

    Article

    van Ham et al FASEB J. 2010 Nov;24(11):4336-42. Epub 2

    Insert

    secreted human annexin 5 (ANXA5 Human)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. OSCA2.3

    Plasmid

    #136595

    Purpose

    Expresses the mechanosensitive channel OSCA2.3 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    OSCA2.3 (HYP1 Mustard Weed)
    

    Use

    Tags

    pIRES2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCB-CBEv2

    Plasmid

    #221139

    Purpose

    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq-Ptac

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-gRNA-puro

    Plasmid

    #180426

    Purpose

    Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

    Insert

    gRNAscr
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 35S::Cel1SP-GeNL/pCAMBIA1301

    Plasmid

    #190069

    Purpose

    Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

    Depositor

    Takeharu Nagai

    Article

    Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

    Insert

    Cel1 signal peptide-Green-enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRJPaph-lacZYA

    Plasmid

    #67037

    Purpose

    Plasmid for Paph-lacZYA integration downstream of B. diazoefficiens (B. japonicum) scoI

    Depositor

    Julia Vorholt

    Article

    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

    Inserts

    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    lacZYA
    

    Use

    Conjugative bacterial vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRJPaph-gusA

    Plasmid

    #67036

    Purpose

    Plasmid for Paph-gusA integration downstream of B. diazoefficiens (B. japonicum) scoI

    Depositor

    Julia Vorholt

    Article

    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

    Inserts

    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    gusA
    

    Use

    Conjugative bacterial vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. A3A-PBE-ΔUGI

    Plasmid

    #119770

    Purpose

    Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-ΔUGI-NLS
    

    Use

    Tags

    NLS

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRJPaph-mTq2

    Plasmid

    #67031

    Purpose

    Plasmid for Paph-mTurqoise2 integration downstream of B. diazoefficiens (B. japonicum) scoI

    Depositor

    Julia Vorholt

    Article

    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

    Inserts

    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    mTurqoise2
    

    Use

    Conjugative bacterial vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJ414-PCY1

    Plasmid

    #98150

    Purpose

    Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRJPaph-HcRed

    Plasmid

    #67035

    Purpose

    Plasmid for Paph-HcRed integration downstream of B. diazoefficiens (B. japonicum) scoI

    Depositor

    Julia Vorholt

    Article

    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

    Inserts

    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    HcRed
    

    Use

    Conjugative bacterial vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pETM6_Ptrc_BsMdh_BmHps_BmPhi

    Plasmid

    #129104

    Purpose

    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

    Insert

    BsMDH BmHPS BmPHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pYPQ265L2

    Plasmid

    #174000

    Purpose

    Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-dLbCpf1-BE-EE

    Plasmid

    #154147

    Purpose

    Expresses dLbCpf1-BE-EE(dLbCas12a-BE-EE) in mammalian cells.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

    Insert

    dLbCpf1-BE-EE (dCas12a-BE-EE)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    APOBEC1(R126E+R132E), dLbCpf1(D832A+E925A+D1148A)

    Promoter

    CMV

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Mdn1-deltaC

    Plasmid

    #171603

    Purpose

    Expresses S. pombe Mdn1 a.a. 1-3911 in insect cells.

    Depositor

    Tarun Kapoor

    Article

    Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

    Insert

    Mdn1
    

    Use

    Tags

    His6 tag

    Expression

    Insect

    Mutation

    C-terminal deletion; a.a. 1-3911 only

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEX-4T-3-cytXlATL2-R232Q

    Plasmid

    #124064

    Purpose

    Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

    Depositor

    Tom Rapoport

    Article

    Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

    Insert

    cytXlATL2-R232Q
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pProEx-Htb-cytATL2-6Gly-sfGFP

    Plasmid

    #124065

    Purpose

    E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    cytATL-sfGFP
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHAGE2 Lnp-mCherry

    Plasmid

    #86687

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    LNPK (LNPK Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 myc-RNF114

    Plasmid

    #58295

    Purpose

    mammalian expression of myc tagged RNF114

    Depositor

    Francesca Capon

    Article

    Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

    Insert

    RNF114 (RNF114 Human)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pCCM’

    Plasmid

    #162709

    Purpose

    pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    p15A origin replaced with high-copy colE1 origin

    Promoter

    PLtet0-1 promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHAGE2 mCherry-Atl1

    Plasmid

    #86678

    Purpose

    Lentivirus to stably express fluorescent protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    ATL1 (ATL1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMW96

    Plasmid

    #178066

    Purpose

    Expression of His6-SUMO-TEV-GFP-PreScission-γ-TuNA in E. coli

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al Cell. 2020 Jan 9;180(1):165-175.e16. doi

    Insert

    CDK5RAP2 (CDK5RAP2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHAGE2 GFP-Atl1

    Plasmid

    #86679

    Purpose

    Lentivirus to stably express fluorescent protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    ATL1 (ATL1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti-eGFP-dSaCas9-blast

    Plasmid

    #191653

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    eGFP-T2A-dSaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-dLbCpf1-BE-YE1

    Plasmid

    #154145

    Purpose

    Expresses dLbCpf1-BE-YE1(dLbCas12a-BE-YE1) in mammalian cells.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

    Insert

    dLbCpf1-BE-YE1 (dCas12a-BE-YE1)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y+R126E), dLbCpf1(D832A+E925A+D1148A)

    Promoter

    CMV

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti-dSaCas9-KRAB-blast

    Plasmid

    #191654

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    dSaCas9-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGEX-HCF-1rep1E10A

    Plasmid

    #84607

    Purpose

    Plasmid contains HCF-1rep1E10A with GST-tag

    Depositor

    Winship Herr

    Article

    Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

    Insert

    HCF-1rep1E10A (HCFC1 Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGEX-HCF-1rep1wt

    Plasmid

    #84606

    Purpose

    Plasmid contains HCF-1rep1wt with GST-tag

    Depositor

    Winship Herr

    Article

    Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

    Insert

    HCF-1rep1wt (HCFC1 Human)
    

    Use

    Tags

    GST-TEV and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pT/Caggs-NRAS G12V-IRES-ASK1

    Plasmid

    #221074

    Purpose

    Expresses NRAS G12V and ASK1 in mammalian cells

    Depositor

    Hidenori Ichijo

    Article

    Odawara et al Commun Biol. 2024 Jun 5;7(1):691. doi: 1

    Inserts

    NRAS (NRAS Human)
    ASK1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HA, EGFP Spastin M87 with exon 4, N386C

    Plasmid

    #167543

    Purpose

    For doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4, with N386C mutation.

    Depositor

    Tarun Kapoor

    Article

    Cupido et al Nat Chem Biol. 2019 May;15(5):444-452. d

    Insert

    Spastin M87 with exon 4 (SPAST Human)
    

    Use

    Tags

    EGFP and HA tag

    Expression

    Mammalian

    Mutation

    N386C

    Promoter

    CMV

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV-SPORT6-RNF114-RINGmut

    Plasmid

    #69922

    Purpose

    Expresses mutant myc-RNF114 in mammalian cells

    Depositor

    Francesca Capon

    Article

    Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

    Insert

    RNF114 (RNF114 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C29A, C32A

    Promoter

    CMV

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

41. pACEBac1-beta-actin

    Plasmid

    #178076

    Purpose

    Insect cell expression of beta-actin

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    beta-actin (ACTB Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. SNAP-MIDAS

    Plasmid

    #171604

    Purpose

    Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.

    Depositor

    Tarun Kapoor

    Article

    Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

    Insert

    MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
    

    Use

    Tags

    His6 tag and SNAP tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCMV-SPORT6-RNF114-UIMmut

    Plasmid

    #69923

    Purpose

    Expresses mutant myc-RNF114 in mammalian cells

    Depositor

    Francesca Capon

    Article

    Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

    Insert

    RNF114 (RNF114 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S224P

    Promoter

    CMV

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

44. pYPQ269A2

    Plasmid

    #164714

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V04 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    rAPOBEC1-zCas9(D10A)-UGI-T2A-MS2-UGI
    

    Use

    CRISPR; Gateway compatible rapobec1-cbe_v04 entry…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pYPQ265

    Plasmid

    #164712

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    rAPOBEC1-zCas9(D10A)-UGI
    

    Use

    CRISPR; Gateway compatible rapobec1-cbe_v01 entry…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc

    Plasmid

    #129105

    Purpose

    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone production

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

    Insert

    BsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

47. Zebrafish-gRNA-0001

    Plasmid

    #42241

    Purpose

    gRNA targeted to zebrafish gene apoea

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    gRNA-apoea (apoea Zebrafish)
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

48. pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP

    Plasmid

    #205603

    Purpose

    Tet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

    Depositor

    Tarun Kapoor

    Article

    Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

    Insert

    γ-tubulinWT-RNAi-resistant-GFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G2228A, C2231T, C2237T, G2240A

    Promoter

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP

    Plasmid

    #205604

    Purpose

    Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

    Depositor

    Tarun Kapoor

    Article

    Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

    Insert

    γ-tubulinΔGTP-RNAi-resistant-GFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G2228A, C2231T, C2237T, G2240A, N229A

    Promoter

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pLenti-Sa-gRNA-puro

    Plasmid

    #191652

    Purpose

    Lentiviral expression of puromycin resistance gene and S. aureus scrambled non-targeting gRNA ; useful for changing gRNA by mutagenesis.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    S. aureus gRNAscr
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge