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Showing: 851 - 900 of 30762 results

  7. pFNC-3

    Plasmid
    #233296
    Purpose
    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Inserts
    BxbI phage integrase
    sacB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PEM7 and unknown
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    233296-plasmid-map-sequence-id-467796

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  9. pREDCas9

    Plasmid
    #71541
    Purpose
    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    lambda red genes
    plasmid curing system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71541-plasmid-map-sequence-id-333602

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  16. pTS2340_Tier3(SB)-iRFP_Puro

    Plasmid
    #169638
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven iRFP670 and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    169638-plasmid-map-sequence-id-364985

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  20. pTS2362_Tier3(SB)-rtTA-PuroR

    Plasmid
    #169658
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169658-plasmid-map-sequence-id-365631

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  25. pTS2303-Tier1-CoTC-PPGK-NLuc_TB

    Plasmid
    #169548
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    CoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169548-plasmid-map-sequence-id-364524

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  31. pBbE1k-3

    Plasmid
    #71722
    Purpose
    tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human
    Depositor
    Taek Soon Lee
    Article
    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1
    Inserts
    dihydropterine reductase (QDPR Human)
    pterin-4a-carbinolamine dehydratase (PCBD1 Human)
    Use
    Tags
    Expression
    Mutation
    Promoter
    pTrc
    Available since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    71722-plasmid-map-sequence-id-129984

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  38. pCas9-beta-mmr

    Plasmid
    #169241
    Purpose
    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations
    Depositor
    Brian Pfleger
    Article
    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:
    Inserts
    cas9
    beta
    mutL-E36K
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Changed glutamic acid 36 to lysine
    Promoter
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169241-plasmid-map-sequence-id-333575

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  40. p168

    Plasmid
    #62618
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62618-plasmid-map-sequence-id-103064

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  42. pTS2337_Tier3(SB)-TagBFP2_Puro

    Plasmid
    #169635
    Purpose
    Tier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven mTagBFP2 and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169635-plasmid-map-sequence-id-366786

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  43. Keasling-2187

    Plasmid
    #100167
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    MevT and MBIS operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lacUV5
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100167-plasmid-map-sequence-id-193500

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  48. JBEI-3716

    Plasmid
    #100169
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    codon-optimized MK
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100169-plasmid-map-sequence-id-193493

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  49. p148

    Plasmid
    #62616
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62616-plasmid-map-sequence-id-102932

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Showing: 851 - 900 of 30762 results