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Showing: 851 - 900 of 32226 results

  1. pTS2355_Tier3(PB)-iRFP_PuroR

    Plasmid
    #169654
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven iRFP670 and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169654-plasmid-map-sequence-id-365633

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  6. pFNC-3

    Plasmid
    #233296
    Purpose
    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Inserts
    BxbI phage integrase
    sacB
    Expression
    Bacterial
    Promoter
    PEM7 and unknown
    Available Since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    233296-plasmid-map-sequence-id-467796

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  7. pTS2340_Tier3(SB)-iRFP_Puro

    Plasmid
    #169638
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven iRFP670 and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    169638-plasmid-map-sequence-id-364985

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  8. pTS2362_Tier3(SB)-rtTA-PuroR

    Plasmid
    #169658
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169658-plasmid-map-sequence-id-365631

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  12. pTS2303-Tier1-CoTC-PPGK-NLuc_TB

    Plasmid
    #169548
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    CoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Expression
    Mammalian
    Promoter
    PPGK
    Available Since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169548-plasmid-map-sequence-id-364524

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  19. p168

    Plasmid
    #62618
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62618-plasmid-map-sequence-id-103064

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  21. Keasling-2187

    Plasmid
    #100167
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    MevT and MBIS operon
    Expression
    Bacterial
    Promoter
    lacUV5
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100167-plasmid-map-sequence-id-193500

    Enlarge

  24. pTS2337_Tier3(SB)-TagBFP2_Puro

    Plasmid
    #169635
    Purpose
    Tier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven mTagBFP2 and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169635-plasmid-map-sequence-id-366786

    Enlarge

  25. pChlr-8CQ

    Plasmid
    #52171
    Purpose
    Encodes module 8 with amino acids 12C-16Q for recognition of nt 1A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C in module 8
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. JBEI-3716

    Plasmid
    #100169
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    codon-optimized MK
    Expression
    Bacterial
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100169-plasmid-map-sequence-id-193493

    Enlarge

  27. p148

    Plasmid
    #62616
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62616-plasmid-map-sequence-id-102932

    Enlarge

  32. pTS2353_Tier3(PB)-YPet_PuroR

    Plasmid
    #169652
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven YPet and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169652-plasmid-map-sequence-id-366196

    Enlarge

  33. pChlr-8SE

    Plasmid
    #52172
    Purpose
    Encodes module 8 with amino acids 12S-16E for recognition of nt 1G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in module 8
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  34. pChlr-1SE

    Plasmid
    #52140
    Purpose
    Encodes module 1 with amino acids 12S-16E for recognition of nt 8G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 25-60 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 16Q to 16E in Module 1
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. pChlr-2SE

    Plasmid
    #52144
    Purpose
    Encodes module 2 with amino acids 12S-16E for recognition of nt 7G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 61-96 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in Module 2
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  36. pChlr-3SE

    Plasmid
    #52148
    Purpose
    Encodes module 3 with amino acids 12S-16E for recognition of nt 6G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 97-132 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12S and 16Q to 16E in Module 3
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. pChlr-4SYR

    Plasmid
    #52158
    Purpose
    Encodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S, 13H to 13Y, and 16Q to 16R in…
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  38. pChlr-5SR

    Plasmid
    #52162
    Purpose
    Encodes module 5 with amino acids 12S-16R for recognition of nt 4C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12S and 16Q to 16R in Module 5
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  39. pChlr-6SE

    Plasmid
    #52164
    Purpose
    Encodes module 6 with amino acids 12S-16E for recognition of nt 3G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in Module 6
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc

    Plasmid
    #129105
    Purpose
    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone production
    Depositor
    Eleftherios Terry Papoutsakis
    Article
    Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10
    Insert
    BsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC
    Expression
    Bacterial
    Available Since
    Nov. 11, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129105-plasmid-map-sequence-id-255085

    Enlarge

  42. pChlr-4SE

    Plasmid
    #52152
    Purpose
    Encodes module 4 with amino acids 12S-16E for recognition of nt 5G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in Module 4
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  43. pChlr-8SR

    Plasmid
    #52174
    Purpose
    Encodes module 8 with amino acids 12S-16R for recognition of nt 1C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16R in Module 8
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  44. pChlr-1SR

    Plasmid
    #52142
    Purpose
    Encodes module 1 with amino acids 12S-16R for recognition of nt 8C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 25-60 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 16Q to 16R in Module 1
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  45. pChlr-7CQ

    Plasmid
    #52167
    Purpose
    Encodes module 7 with amino acids 12C-16Q for recognition of nt 2A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 241-283 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12S to 12C and 16E to 16Q in Module 7
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  46. pChlr-7NQ

    Plasmid
    #52169
    Purpose
    Encodes module 7 with amino acids 12N-16Q for recognition of nt 2U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 241-283 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12S to 12N and 16E to 16Q in module 7
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  47. pChlr-7SYR

    Plasmid
    #52170
    Purpose
    Encodes module 7 with amino acids 12S-13Y-16R for recognition of nt 2C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 241-283 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 13N to 13Y and 16E to 16R in module 7
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  48. pChlr-1NQ

    Plasmid
    #52141
    Purpose
    Encodes module 1 with amino acids 12N-16Q for recognition of nt 8U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 25-60 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12S to 12N in Module 1
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  49. pChlr-2CQ

    Plasmid
    #52143
    Purpose
    Encodes module 2 with amino acids 12C-16Q for recognition of nt 7A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 61-96 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C in Module 2
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  50. pChlr-2SR

    Plasmid
    #52146
    Purpose
    Encodes module 2 with amino acids 12S-16R for recognition of nt 7C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 61-96 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16R in Module 2
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 851 - 900 of 32226 results