We narrowed to 10,430 results for: yeast

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p404-MTS-mCherry-GFP1-10

Plasmid

#91958

Purpose

Expresses mCherry-GFP1-10 in yeast cell mitochondria

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

MTS-mCherry-GFP1-10

Expression

Yeast

Promoter

GAP

Available Since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAGM9121_His2-GFP11

Plasmid

#153511

Purpose

His2-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

His2-GFP11

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pWS5399-CRISPRai-ZeoR

Plasmid

#182838

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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HYG-TET-OFF – ubiquitin – LEU – 4FLAG

Plasmid

#193315

Purpose

Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

Ubiquitin - Leucine - 4FLAG N-terminal tagging

Tags

4FLAG

Expression

Bacterial

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pWS5390-CRISPRai-URA3

Plasmid

#182829

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - URA3 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pJO629

Plasmid

#104342

Purpose

Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

Depositor

Alexander Varshavsky

Article

Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2

Insert

pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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PP244

Plasmid

#78978

Purpose

Promoter: ppTEF1, minimal Promoter: GAP. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pIW447-CRISPR XYL2

Plasmid

#98906

Purpose

K. marxianus CRISPR Plasmid for XYL2 editing

Depositor

Ian Wheeldon

Article

Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

Inserts

Codon optimized Cas9
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Tags

SV40

Expression

Yeast

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a-trAtCCD8(pYL735)

Plasmid

#178286

Purpose

Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

trAtCCD8

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Plasmid

#58440

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pWS5396-CRISPRai-KanR

Plasmid

#182835

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - KanR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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YIPlac204TKC-E2-Crimson-HDEL

Plasmid

#38771

Depositor

Benjamin Glick

Article

Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

Insert

E2-Crimson

Tags

Linker peptide and yeast ER retention signal sequ…

Expression

Yeast

Promoter

TPI1

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pYR69 (X-4:4xUAS-SSA1p-365:yEGFP3-CYC1t)

Plasmid

#167355

Purpose

Integrative plasmid for promoter-reporter 4xUAS-SSA1p-365:yEGFP3-CYC1t

Depositor

Michael Jensen

Article

Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

Insert

4xUAS-SSA1p-365:yEGFP3-CYC1t

Use

Unspecified

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Plasmid

#58439

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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PP78

Plasmid

#78948

Purpose

Supplemental Figure 3. Integration Site 5.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

Integration Site 5

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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dCas9v2

Plasmid

#103140

Purpose

TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

dCas9-VPR

Expression

Yeast

Mutation

Removed scaffold gRNA from original plasmid

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCORE-Kp53

Plasmid

#72241

Purpose

Contains the CORE cassette with KanMX4 and human p53 gene markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

p53 (TP53 Human)
kanMX4

Expression

Bacterial

Mutation

V122A mutation

Available Since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Plasmid

#58441

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 3 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pJL-mGold

Plasmid

#157997

Purpose

Expression of mGold in yeast cells

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

mGold

Expression

Yeast

Mutation

mGold is mVenus with L46F;T63S mutations

Promoter

pTDH(GAP promoter)

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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BSP188

Plasmid

#110917

Purpose

For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

unc54 Terminator

Use

Synthetic Biology; Yeast homologous recombination…

Expression

Worm

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pWS5394-CRISPRai-MET17

Plasmid

#182833

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - MET17 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS8-HaloTagx2

Plasmid

#177113

Purpose

Expresses Vps8-HaloTagx2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

VPS8 (VPS8 Budding Yeast)

Expression

Yeast

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDZ585 pKAN 2x-mCherry

Plasmid

#72236

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherry

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

2x-mCherry

Expression

Bacterial

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG417

Plasmid

#105341

Purpose

Yeast two hybrid

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG418

Plasmid

#105342

Purpose

Yeast two hybrid

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-ySet2

Plasmid

#51698

Purpose

Expresses full length yeast Set 2 gene in a mamalian expression vector

Depositor

Tom Misteli

Article

Luco et al Science. 2010 Feb 19;327(5968):996-1000.

Insert

yeast Set2 (SET2 Budding Yeast)

Tags

C-terminal FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only
PP255

Plasmid

#78934

Purpose

Promoter: scTEF1, minimal Promoter: GAP. Figure 2 and 4.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Dhh1-EGFP

Plasmid

#201418

Purpose

Bacterial protein expression of Dhh1

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dhh1 (DHH1 Budding Yeast)

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Promoter

tac

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD6-pGRR-r11-r10-yeGFP

Plasmid

#74368

Purpose

pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.

Depositor

Eric Klavins

Article

Gander et al bioRxiv 041871

Insert

yeGFP

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

pGRR-r11-r10

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pMAD-Gate2

Plasmid

#25941

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

Insert

Tags

VP16 Fusion Protein

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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