We narrowed to 14,039 results for: TIM

1. pBXNH3LCA_TM287/288

   Plasmid

   #132701

   Purpose

   expresses TM287/288 in E.coli, to use as positive control in ELISA

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   TM287/288 (TM0288, TM0287 Thermotoga maritima)
   

   Tags

   Avi and His

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIII-Neo-tdTomato

   Plasmid

   #153424

   Purpose

   Retroviral (MSCV) expression of tdTomato fluorescent protein

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   tdTomato
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEB2-mRFP1*

   Plasmid

   #104000

   Purpose

   Plasmid encoding mRFP1*

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mRFP1*
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (MSKGEE NNLAVIKEF...T…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMIR-Dsred-IRES-His Keap

   Plasmid

   #62457

   Purpose

   expresses his tagged human Keap1 protein

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Tags

   HisTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. RT3REVIN

   Plasmid

   #111167

   Purpose

   Tet-regulated miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Retroviral

   Tags

   DsRed and mVenus

   Mutation

   WT

   Promoter

   T3G

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS

   Plasmid

   #63170

   Purpose

   GAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-OLLAS"
   

   Tags

   N-myristoylation

   Expression

   Insect

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. GADD45 P-3m-luc

   Plasmid

   #8358

   Depositor

   Michael Greenberg

   Article

   Tran et al Science 2002 Apr 19;296(5567):530-4.

   Insert

   GADD45 promoter P-3m (GADD45A Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   P-3m

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

8. ScFv-2ERT2-V

   Plasmid

   #120553

   Purpose

   3rd gen transfer vector. Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP, 2 tandem ERT2 and VP64.

   Depositor

   Yu Wang

   Article

   Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

   Insert

   scFvGCN4, sfGFP, GB1, ERT2, VP64
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPS0384

   Plasmid

   #133234

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   April 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEB2-mRFP1

    Plasmid

    #104001

    Purpose

    Plasmid encoding mRFP1

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mRFP1
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWL502-crRNA

    Plasmid

    #174383

    Purpose

    The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei

    Depositor

    Jing Han

    Article

    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

    Insert

    a crRNA targeting the template strand
    

    Use

    CRISPR; Archaeal expression

    Expression

    Bacterial

    Promoter

    PhaR promoter

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.2/2K-NS4B

    Plasmid

    #120827

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    2K-NS4B ZIKV
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB80-FRB-GFP-ppKin14VIb(861-1321)

    Plasmid

    #174638

    Purpose

    Rapalog-inducible coupling to dimeric moss kinesin-14 for retrograde transport via FRB

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    FRB-GFP-ppKin14VIb(861-1321)
    

    Tags

    FRB-GFP

    Expression

    Mammalian

    Mutation

    EGFP: Met1Del; ppKin14VIb(aa861-1321)

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.2/NS4A-2K

    Plasmid

    #120820

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS4A + 2K ZIKV
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBI-CMV1-mir421-CMV2-mir1

    Plasmid

    #164673

    Purpose

    Express miR-421 and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-421 (MIR421 Human)
    miR-1-1 (MIR1-1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDY-HA1-HA2-neo

    Plasmid

    #109123

    Purpose

    FR cassette provider to introduce FR cassette to a BAC by recombineering

    Depositor

    David Gilbert

    Article

    Sima et al Nucleic Acids Res. 2018 Feb 28;46(4):181

    Inserts

    HA1
    HA2
    

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOPS0385

    Plasmid

    #133235

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with NoP (pOGG113), gusA (pOGG083) and T-pharma (pOGG003)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOPS0386

    Plasmid

    #133236

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with NoP (pOGG113), celB (pOGG050) and T-pharma (pOGG003)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOPS0383

    Plasmid

    #133233

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with NoP (pOGG113), sfGFP (pOGG037) and T-pharma (pOGG003)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-HsSENP5

    Plasmid

    #71468

    Purpose

    Encodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 5 (SENP5 Human)
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only