169,691 results
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Plasmid#53116Purposeexpresses alpha hemolysin under the control of theophylline riboswitchDepositorInserttheophylline riboswitch and alpha hemolysin
ExpressionBacterialPromoterT7 promoterAvailable SinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
EF1a_CDX2_P2A_Hygro_Barcode
Plasmid#120432PurposeBarcoded lentiviral vector to express CDX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nrxn1beta AS4(+)-Fc
Plasmid#59314PurposeExpression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]DepositorInsertNrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)
TagsFc (from human IgG)ExpressionMammalianPromoterCMV, T7Available SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-HA-ERK2 GOF
Plasmid#53174PurposeExpresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vectorDepositorInsertERK2 (MAPK1 Human, Mustard Weed)
UseRetroviralTagsHAExpressionMammalianMutationR67S/D321NAvailable SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLENTI-CMV-GFP-TREX1 delta C (aa1-235) BLAST
Plasmid#164229Purposelentiviral plasmid for GFP-hTREX1 delta C (aa1-235)DepositorInsertTREX1 (TREX1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationdeleted amino acids 235-314PromoterCMVAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
scFv-GCN4-DNMT3a-DNMT3l
Plasmid#154140PurposeExpresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.DepositorInsertscFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
TagsHA and sfGFPExpressionMammalianPromoterSFFVAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EGFP-T2A-ACREB-WPRE
Plasmid#40867DepositorInsertACREB
UseAAVPromoterCMVAvailable SinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp
Plasmid#101349Purpose2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3DepositorInsertNL4-3
UseLentiviralMutationdelta tat delta TAR delta env GFP in Nef Tet ope…Available SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL1_37
Plasmid#199044PurposeLevel 1 plasmid, barcoded ORIDepositorInsertRSF1010 ORI plus NGS barcode 14
UseSynthetic BiologyAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II KS(+) ACMV DNA-A 1.4mer
Plasmid#159134PurposeInfectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-ADepositorInsertACMV DNA-A 1.4mer
UseInfectious clone for bombarding plantsAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mDIS3-FLAG
Plasmid#60046PurposeMammalian expression of FLAG-tagged mouse DIS3DepositorAvailable SinceNov. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
Gi-c001
Plasmid#173078PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL0_168 [pMB1 ORI]
Plasmid#210687PurposeLevel 0 partDepositorInsertpMB1 ORI
UseSynthetic BiologyAvailable SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR
Plasmid#136382PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion proteinDepositorInsertAaCas12b (D570A)-TV-T2A-MCP-VPR
UseCRISPRExpressionPlantMutationD570AAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV tat-P2A-rev
Plasmid#101340PurposeLTR drives GFP and internal SFFV promoter drives codon optimized tat and rev.DepositorInsertGFP, tat, rev
UseLentiviralAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDB070.iodo.5
Plasmid#99397PurposeExpression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanineDepositorInsertPN25 TetR; Ptet Iodo.5RS; PproK tyrT(Opt,CUA)
ExpressionBacterialPromoterPN25, Ptet, and PproKAvailable SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
miniTurbo-dCasRx
Plasmid#219821PurposeEF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)DepositorInsertdCasRx
UseCRISPRTagsminiTurboExpressionMammalianPromoterEF1a and hU6Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCBE3
Plasmid#133966PurposeExpresses BE3 CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertBE3
UseCRISPR and Synthetic BiologyAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pATZ-BFP-HA-GFP11
Plasmid#177721PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
TagsGFP11, HA, and TagBFPExpressionBacterial and MammalianMutationE342K (ATZ mutant)Available SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-DFCP1
Plasmid#86746PurposeN-terminal mCherry-tagged protein expression in mammalian cellsDepositorAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only