163,606 results

1. pL1P4 ZmUbiP:GRF-GIF:NosT

   Plasmid

   #198048

   Purpose

   MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

   Depositor

   Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

   Insert

   ZmUbiP::TaGRF4-GIF1::NosT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Zea mays (maize) ubiquitin promoter

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Hygro

   Plasmid

   #127763

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lv EF1a dCas9-HA 2A Blast

   Plasmid

   #102812

   Purpose

   Lentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion protein

   Depositor

   Jerry Crabtree

   Article

   Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

   Insert

   dCas9-HA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A (dCas9)

   Promoter

   EF1a

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

   Plasmid

   #175547

   Purpose

   Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag7 and Tomm20-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-VSVM

   Plasmid

   #64086

   Purpose

   Expression vector for Vesicular Stomatitis Virus Indiana strain matrix gene

   Depositor

   Ian Wickersham

   Insert

   VSV matrix protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Arl13b-EGFP

   Plasmid

   #234844

   Purpose

   A mammalian expression plasmid encoding Arl13b with EGFP at C-terminus.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Arl13b (ARL13B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3/er-(n2)oxStayGold(c4)v2.0

   Plasmid

   #186296

   Purpose

   A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.

   Depositor

   Atsushi Miyawaki

   Article

   Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

   Insert

   er-(n2)oxStayGold(c4)v2.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET30-2-GAPDH

   Plasmid

   #83910

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
   

   Use

   Tags

   6x His tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_CBFB_WT

   Plasmid

   #81929

   Purpose

   Gateway Donor vector containing CBFB, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   CBFB (CBFB Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-CANX

    Plasmid

    #227279

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of CANX (CANX Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRT029

    Plasmid

    #127969

    Purpose

    double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTubulin-Electra2

    Plasmid

    #184930

    Purpose

    Tubulin labeling with blue fluorescent protein Electra2

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    TUBA1B-Electra2
    

    Use

    Tags

    Electra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL4393 (pClpX_Eco)

    Plasmid

    #200907

    Purpose

    Expresses human codon-optimized E. coli ClpX from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    EcoClpX
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234453

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV; Flp/frt

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLNCX2 ER:ras

    Plasmid

    #67844

    Purpose

    4-hydroxytamoxifen inducible ER:ras fusion protein

    Depositor

    Masashi Narita

    Article

    Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

    Insert

    ER1a H-RasG12V fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G525R renders the HBD largely insensitive to 17ß‐…

    Promoter

    CMV

    Available Since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2 Notch1 Full Length-6MT

    Plasmid

    #41728

    Depositor

    Raphael Kopan

    Article

    Schroeter et al Nature. 1998 May 28;393(6683):382-6.

    Insert

    Notch-1 Full Length (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    L1997V; Contains murine Notch-1 protein up to ami…

    Promoter

    Available Since

    Dec. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA

    Plasmid

    #105648

    Purpose

    Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks

    Depositor

    Sandra Rieger

    Insert

    Tol2-Isl2b:GFP:polyA (isl2b Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS31-cCARLIN

    Plasmid

    #160928

    Purpose

    Contains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)

    Depositor

    Fernando Camargo

    Article

    Bowling et al Cell. 2020 Jun 11;181(6):1410-1422.e27.

    Insert

    CARLIN gRNAs and CAG-GFP-CARLIN array
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for gRNAs; CAG promoter for GFP

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVT100U-mtGFP

    Plasmid

    #45054

    Depositor

    Benedikt Westermann

    Article

    Westermann et al Yeast. 2000 Nov;16(15):1421-7.

    Insert

    mtGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available Since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP SMG1 WT

    Plasmid

    #199571

    Purpose

    Expresses WT SMG1 fused to GFP

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    SMG1 WT (SMG1 Human)
    

    Use

    Tags

    GFP fusion protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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