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Plasmid#228487Purpose10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expressionDepositorTypeEmpty backboneUseTags10xHis-SUMO(Smt3)-GST-HRVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only
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pGP-Tn7-Pcym
Plasmid#225280PurposeMini-Tn7 pGP-Tn7-Cm vector with inducible Pcym cumate promoter for the inducible/controlled expression of target genes inserted into the attTn7 chromosomal site.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPcym cumate (p-isopropylbenzoate)-inducible promo…Available sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-T4uvsX
Plasmid#228213PurposeExpression in BL21(DE3) of T4 recombinaseDepositorInsertuvsX (uvsX Escherichia phage T4)
UseTagsSce VMA intein-chitin binding domainExpressionBacterialMutationPromoterT7Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
TagBFP(S2)2(S4)2-gs
Plasmid#228072PurposeMammalian expression of coiled coil segments (S2)x2-(S4)x2, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertBFP, S2, S4
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-CHMP4B gRNA2
Plasmid#211629PurposesgRNA-2 against CHMP4BDepositorInsertsgRNA CHMP4B
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-CHMP4B gRNA1
Plasmid#211628PurposesgRNA-1 against CHMP4BDepositorInsertsgRNA CHMP4B
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
G421C_BattB(GA)_mCherry
Plasmid#200633PurposeStrong mCherry fluorescence recombination plasmid for Matreyek Bxb1(GA) landing padDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOttc1472 - pAAV CaMKII KDELR1-Myc-DDK
Plasmid#192599PurposeAn AAV packaging vector that expresses KDELR1 under control of the CaMKII promoter.DepositorInsertKDELR1 (KDELR1 Human)
UseAAVTagsExpressionMutationPromoterCaMKIIAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2098
Plasmid#141921PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZKSCAN1 (ZKSCAN1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPPC019.AAV
Plasmid#171147PurposeExpression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmidDepositorInsertshAAVS1 scRNA
J1-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase INF enhancer vector_ORI_MX1
Plasmid#99323PurposeLuciferase validation vector with MX1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr21: 42791443 -42793515
UseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX307 P2A GFP Luciferase
Plasmid#193674PurposeConstitutive lentiviral expression of LuciferaseDepositorInsertLuciferase
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PARP1
Plasmid#215136PurposeExpresses mCherry-PARP1 in mammalian cells from a retroviral vector.DepositorInsertPARP1 (PARP1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5154
Plasmid#175390PurposeHigh Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)DepositorTypeEmpty backboneUseMouse Targeting; Cloning vector, to be used to de…TagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-Brainbow-2.1 R
Plasmid#18723DepositorInserthrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET21a-LysRS-His
Plasmid#124114PurposeBacterial expression plasmid for PURE system componentsDepositorInsertLysine-tRNA ligase (lysU Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVS1-VIR2
Plasmid#134745PurposeVirulence and replication helperDepositorInsertvir gene cluster
UseVir gene expressionTagsExpressionMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
DRD2-3xHA/pCDNA3.1
Plasmid#196940PurposePlasmid expressing an antigen targeting the D2 dopamine receptorDepositorInsertDRD2 (DRD2 Human)
UseTags3xHAExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX208 CMV sTurboID (C)-HA-FRB-ERM
Plasmid#153007PurposeExpresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membraneDepositorInsertsTurboID (C) (G74)-ERM (Cb5)
UseLentiviralTagsFRB and HAExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lambda Phosphatase
Plasmid#79748PurposeIntended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.DepositorInsertLambda Phosphatase
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only