We narrowed to 10,522 results for: SEC
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Plasmid#39825DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_175_Venus_195
Plasmid#39831DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
U6b-T
Plasmid#117210PurposeExpresses gRNA in Aedes aegyptiDepositorInsertu6b promoter-gRNA
UseSynthetic BiologyExpressionInsectPromoterU6Available SinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTF3-AK (clone3)
Plasmid#21165DepositorInsertPOUF5F1 (genomic DNA) (POU5F1 Human)
UseCre/Lox; Homologous recombinationMutationNeoR, EGFP, and SV40 polyA are between the two ge…Available SinceJune 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
TOPO CCSP
Plasmid#68453PurposeTOPO construct expressing CCSP for generation of GMAP-compatible promoterDepositorInsertClara Cell Secretory Protein Promoter
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-R896K::NG
Plasmid#138957PurposeUsed to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv-R896K::NG (kkv Fly)
TagsNeon Green fused to C terminus of KkvExpressionInsectMutationR896KPromoterUASAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAct5C-Hh
Plasmid#37382DepositorAvailable SinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-sGFP
Plasmid#79508PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Super GFP CDSDepositorInsertsuperGFP
UseMultisite gateway entry cloneTags6xHisPromoterno PromoterAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_227_Venus_173
Plasmid#39835DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASTattb-cAMPr Y205A, R626A
Plasmid#84932PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.DepositorInsertcAMPr Y205A R626A
ExpressionInsectMutationPKA-C Y205A, PKA-R R626APromoter5X UASAvailable SinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHD-mW-attPCC-FRT (pTL717)
Plasmid#115158PurposeVector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.DepositorInsertmW attP-CC FRT
ExpressionInsectAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.scr_2
Plasmid#190704PurposeExpresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertScrambled sgRNA 2
UseCRISPRExpressionInsectPromoterDrosophila U6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK_ToeRepG2_N02_trigger
Plasmid#132745PurposeT7 RNAP-driven expression of second-generation toehold repressor index 2 trigger RNADepositorInsertToeRepG2_N02_trigger
ExpressionBacterialAvailable SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac K-M33
Plasmid#1986DepositorAvailable SinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGL3-DSCP-Gateway
Plasmid#71507PurposeflySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the DSCP core promoterDepositorTypeEmpty backboneUseFlystarr-seq validation vectorTagsluciferaseExpressionInsectAvailable SinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-OSF-TRIM5-21R
Plasmid#79063PurposeGateway entry vector for cloning and propagation in DH5alphaDepositorInsertOSF-TRIM5-21R
TagsOneSTrEP-FLAGExpressionInsectPromoterT7Available SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-scFv
Plasmid#78900PurposeExpresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cellsDepositorInsertscFV-VP64
UseCRISPRTagsGFPExpressionInsectPromoterpActin (Drosophila)Available SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD)
Plasmid#176199PurposeEncodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasmDepositorInsertgfcD
TagspelC leader-6xHisExpressionBacterialPromoterT7Available SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD-10xHis)
Plasmid#176200PurposeEncodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasmDepositorInsertgfcD
TagspelC leader-10xHisExpressionBacterialPromoterT7Available SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-His-VHL-Flag
Plasmid#29497DepositorAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HFW-CRY2-VHH(GFP)
Plasmid#126027Purposeexpress Flag-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHR-VHH(GFP)
TagsFlagExpressionInsectMutationWTPromoterHsp70Available SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-tgRNA-nlsBFP
Plasmid#169029PurposegRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6:3Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJUMP27ts[mCherry]-1A(lacZ)
Plasmid#126980PurposeLevel 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationRepA A56V thermosensitive mutationAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B
Plasmid#176665PurposeExpression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-WingGFP-tan
Plasmid#83473PurposeEGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.DepositorInsertWingGFP-tan
UseCRISPRExpressionBacterial and InsectPromoterHsp70 and enhancer from D. melanogaster yellow ge…Available SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab4 DN
Plasmid#37691DepositorInsertRab4 (Rab4 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS22N (dominant negative)PromoterGAL4Available SinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmEGFP-C
Plasmid#113751PurposeGateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags3xmEGFPAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His-Flag-Tipin
Plasmid#22890DepositorAvailable SinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLib6.6B
Plasmid#176666PurposeExpression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGE-attBCC-FRT-mW (pTL788)
Plasmid#115159PurposeIntegration vector for phiC31-mediated transgenesis using a genomic attP-CC site. Contains an attB-CC site, the visible GMR-white flanked by a FRT site for FLP-mediated removal.DepositorInsertattB-CC FRT
ExpressionInsectAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_wt_Venus_157
Plasmid#39814DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT eGFP-RVFV NSs HhRz
Plasmid#91801PurposeExpresses a non-polyadenylated, translatable EGFP mRNA in DrosophilaDepositorInsertEGFP
ExpressionInsectPromoterMetallothionein Promoter (pMT)Available SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRIV-Gal4DBD:2xnMagHigh1
Plasmid#182031PurposeDonor plasmid for conversion of KO attP Drosophila lines into ShineGal4 driversDepositorInsertGal4DBD2xHighMag1-p103'UTR
UseDonor plasmidExpressionInsectAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Egl-Trbo
Plasmid#179993PurposeExpresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.DepositorInsertEgl-Trbo
TagsTurboIDExpressionInsectPromoterUAS-Gal4Available SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Glt1b
Plasmid#98706Purposemammalian expression of Glt1bDepositorInsertGlt1b (Slc1a2 Rat)
ExpressionMammalianMutationI348V and N365Y (please see depositor comments se…PromoterCMVAvailable SinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
BacPAK Flag-NFRKB
Plasmid#19791DepositorAvailable SinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design2_mTFP_105_Venus_195
Plasmid#40002DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-mos::NG
Plasmid#138958PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4DepositorArticleInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectMutation8 bp that are in the region that encodes the cata…PromoterUASAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmRuby-N
Plasmid#113754PurposeGateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags3xmRuby2Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC
Plasmid#71812PurposeGenerating transgenic flies for FLP-inducible DamIDDepositorInsertmin.hsp70P-FRT-STOP#1-FRT-Dam-Myc-PC
ExpressionInsectAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only