We narrowed to 35,392 results for: CaS;
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Plasmid#105983PurposeThis AAV plasmid in the presence of Cre recombinase expresses an extracellular SYFP2 fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.DepositorInsertFLEX-SYFP2-POST-T2A-dTomato.FLEX
UseAAV and Cre/LoxTagsSYFP2-POST, T2A-dTomato, and mycExpressionMammalianPromoterhuman Synapsin 1Available SinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE hygro MEN1 A242V
Plasmid#11022DepositorAvailable SinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
TKTL1
Plasmid#72419PurposeStudy the role of aberrant expression of TKTL1 in HNSCC tumorigenesisDepositorInsertTKTL1 (TKTL1 Human)
ExpressionMammalianMutationmay have one or two synonymous substitutionsPromoterCMVAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED4
Plasmid#15425DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHS1859
Plasmid#205972PurposeFLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorInsertCsy3/Cas7 from JAGBLH010000220, Cas8-HNH
TagsFLAG-SV40 NLSExpressionMammalianPromoterCMVAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1860
Plasmid#205973PurposeFLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorInsertCas6/Csy4 from JAGBLH010000220, Cas8-HNH
TagsFLAG-SV40 NLSExpressionMammalianPromoterCMVAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-MICU1-mEF
Plasmid#31765DepositorInsertMICU1 (MICU1 Human)
UsePdonr gatewayTagsNoneMutationMutated in both EF-hand domains: D231A, E242K, D4…Available SinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBUN501
Plasmid#50582PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistanceDepositorInsertszCas9D10A
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG and NLSMutationCas9D10A nickase, was derived from the zCas9 and …PromoterAtU6-26p and Ubi1pAvailable SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
MIGR1-CD5
Plasmid#159329PurposeOver-expression of mouse CD5DepositorAvailable SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
AA025
Plasmid#216005PurposeFragmid fragment: (Cas protein) NG PAMDepositorHas ServiceCloning Grade DNAInsertCas9-NG_v1.1 [Sp]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn3
Plasmid#173936PurposeBacterial expression of PARP1 lacking Zn3 domainDepositorInsertPARP1delZn3 (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 214-373; V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
AA180
Plasmid#216023PurposeFragmid fragment: (Cas protein) NGRR(T) PAMDepositorHas ServiceCloning Grade DNAInsertCas9_v1.1 [Sa]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA027
Plasmid#216007PurposeFragmid fragment: (Cas protein) nearly PAM-lessDepositorHas ServiceCloning Grade DNAInsertCas9-SpRY_v1.1 [Sp]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA026
Plasmid#216006PurposeFragmid fragment: (Cas protein) NG PAMDepositorHas ServiceCloning Grade DNAInsertCas9-SpG_v1.1 [Sp]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-KLF7-T2A-PuroR
Plasmid#162346PurposeLentiviral expression of KLF7 under the control of the TetON promoterDepositorAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED10
Plasmid#15417DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
nCre-ST-NLS-P2A-NLS-pSC-cCre
Plasmid#207638PurposeA plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequenceDepositorInsertnCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre
ExpressionMammalianMutationAmber stop codon at SpyCatcher’s critical lysinePromoterCMVAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(WT)
Plasmid#177131PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(WT) with a TEV protease site located between the GST tag and PolB(WT)DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2-P14P5K
Plasmid#79570Purposeexpression of mCherry-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1cDepositorInsertPip5k1c (Pip5k1c Mouse)
TagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationR445E and R446E; truncation of amino acids 636-66…PromoterCMVAvailable SinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcw107
Plasmid#64648Purpose(control) when used to produce lentivirus, express physiological levels of insertDepositorInsertn/a
UseLentiviralPromoterPGKAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-NEUROG3-T2A-PuroR
Plasmid#162341PurposeLentiviral expression of NEUROG3 under the control of the TetON promoterDepositorAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TUBG2
Plasmid#171966PurposeExpression in cell lines of the TUBG2 geneDepositorInsertTUBG2 (TUBG2 Human)
ExpressionMammalianMutationRemoval of the internal TUBG2-EcoRI site by chang…Available SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
BMR1_01g02031-bio-His
Plasmid#108116Purpose[Edit] Expresses enzymatically monobiotinylated full-length BMR1_01g02031 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tagDepositorInsertBMR1_01g02031
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-MCART1K91A
Plasmid#133248PurposeExpress FLAG-tagged MCART1K91A in mammalian cellsDepositorInsertMCART1 (SLC25A51 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationcodon-optimized for expression in human cells, K9…Available SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-dHer1cys
Plasmid#73218PurposeBacterial expression vector for Z1907 affibody protein (A) with N-terminal His10, GST and HRV3C cleavage site (cysteine tag)DepositorInsert10XHis-GST-HRV-dHer1cys
Tags10XHis-GST-HRV and The dimer of the Her1 Affibody…ExpressionBacterialPromoterT7Available SinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHS1801
Plasmid#205966PurposeJAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promotersDepositorInsertCas8-HNH proteins and CRISPR array
ExpressionBacterialPromoterLac (proteins), pJ23119 (crRNA)Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
LB335 (Bcl-2 from ATG to -1281)
Plasmid#15382DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationBcl-2 fragment from ATG to -1281 with P2Available SinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED27
Plasmid#15424DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET41a-MED19
Plasmid#15430DepositorAvailable SinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-AC8(1-106)
Plasmid#181958PurposeResidues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.DepositorInsertEGFP-AC8(1-106) (Adcy8 Rat)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-MAPTau-C-10
Plasmid#55431PurposeLocalization: Microtubules, Excitation: 433, Emission: 475DepositorAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS
Plasmid#158628PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVSDepositorAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn1
Plasmid#173933PurposeBacterial expression of PARP1 lacking Zn1 domainDepositorInsertPARP1delZn1 (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 1-96; V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-HD
Plasmid#174809PurposeBacterial expression of isolated PARP1 HD subdomainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED21
Plasmid#15422DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
FN-Ets2
Plasmid#44603DepositorAvailable SinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)
Plasmid#115137PurposeCMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLSDepositorInserthuman codon optimized AcrVA2.1 anti-CRISPR protein
TagsNLS(SV40)ExpressionMammalianAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
AA007
Plasmid#216002PurposeFragmid fragment: (Cas protein) canonical Cas9; NGG PAMDepositorHas ServiceCloning Grade DNAInsertCas9_v1.1 [Sp]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
F-MED28
Plasmid#15368DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
HcRed-pcw107-V5
Plasmid#64647Purpose(control) when used to produce lentivirus, express physiological levels of insertDepositorInsertn/a
UseLentiviralTagsV5PromoterPGKAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
HT116_pAAV_hSyn-DiO-SomQuasAr6b_EGFP
Plasmid#190879PurposeCre-on expression of soma-targeted QuasAr6b under an neuronal promoterDepositorInsertsoma-targeted QuasAr6b
UseAAVTagsEGFPPromoterhSynAvailable SinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant
Plasmid#149507PurposeTransient expression of human MFSD12 with recoded nucleotide sequence. Dileucine motif LL256-257 has been mutated to AA.DepositorInsertMFSD12 (MFSD12 Human)
UseTransient expressionTagsTEV-3XHA-3XFLAGExpressionMammalianMutationCodon Optimized Sequence, p.LL256-257AAPromoterCMVAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nanog-luciferase targeting construct
Plasmid#26591DepositorInsertNanog promoter-luciferase-lox-pgk-neomycin-loc
ExpressionBacterialAvailable SinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pOTTC814 - pAAV-SYN1-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL
Plasmid#63887PurposeAn AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the SYN1 promoter.DepositorInsertER-localized low-affinity GCaMP3(10.19)
UseAAVPromoterhuman SYN1Available SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40
Plasmid#105982PurposeThis AAV plasmid in the presence of Cre recombinase expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.DepositorInsertFLEX-FAPdL5-POST-T2A-dTomato.FLEX
UseAAV and Cre/LoxTagsFAPdL5-POST, T2A-dTomato, and mycExpressionMammalianPromoterhuman synapsin 1Available SinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Doc2beta C2AB(125) G361C
Plasmid#134694PurposeEncodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labelingDepositorInsertDouble C2 protein beta (Doc2b Rat)
TagsGSTExpressionBacterialMutationG361C, C145A, C217A, C249A, C290A, C338A, C387AAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,Δ42a] mut
Plasmid#26577DepositorInsertCACNA1D (Cacna1d Rat)
ExpressionMammalianMutationContains exons 8a, 11, and 31b.Exons 32 and 42a a…Available SinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
HOXB7 (human) pCMV
Plasmid#8539DepositorInsertHOXB7 (HOXB7 Human)
TagsFLAGExpressionMammalianMutationstarts at AA#1 preceeded by FLAG and Ser-Arg-Ile…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED11
Plasmid#15418DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-Orai1
Plasmid#89818PurposeExpress Orai1 in mammalian cellsDepositorInsertOrai1 (ORAI1 Human)
UseRetroviralAvailable SinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only