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PKC beta II dNPS

Plasmid

#16383

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

N-terminal pseudosubstrate domain deletion: aa 30…

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pMXs-Trib3

Plasmid

#131606

Purpose

expression of mouse Trib3 in mammalian cells

Depositor

Filipe Pereira, Kateri Moore

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Trib3 (Trib3 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-mSox10-2

Plasmid

#37007

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSox10-2 (Sox10 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
Emerald-MannII-N-10

Plasmid

#54150

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

MannII (Man2a1 Mouse)

Use

Tags

Emerald

Expression

Mammalian

Mutation

aa 1-112 of MannII aa 1-112 of MannII

Promoter

CMV

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
3578 pcDNA3 Bad S112A

Plasmid

#8797

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

Insert

Bad (Bad Mouse)

Use

Tags

Expression

Mammalian

Mutation

S112A

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pSECC-sgLKB1-A

Plasmid

#138663

Purpose

Expresses a mouse LKB1-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgLKB1 mouse (Stk11 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Dub3 mouse

Plasmid

#72682

Purpose

mammalian expression of mouse Dub3

Depositor

Domenico Maiorano

Article

van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

Insert

Dub3 (Usp17le Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only
MMTV-VEGF

Plasmid

#44625

Depositor

Robert Oshima

Article

Oshima et al Cancer Res. 2004 Jan 1;64(1):169-79.

Insert

VEGF121 (Vegfa Mouse)

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

contains aa160-368; G165E mutation

Promoter

Mouse mammary tumor virus promoter

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
tdTomato-SSTR3-N-17

Plasmid

#58132

Purpose

Localization: Cilium, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Insert

SSTR3

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
PB-hSyn-mSyp-RT1-24xGCN4

Plasmid

#106300

Purpose

The plasmid encodes mouse Synaptophysin sparsely labeled with SunTag24x_v4.

Depositor

James Zhe Liu

Article

Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

Insert

mSyp-RT1-24xGCN4 (Syp Mouse)

Use

Tags

24xGCN4

Expression

Mammalian

Mutation

Promoter

human Synapsin1

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLV V5-TbID-emerin

Plasmid

#175099

Purpose

Lentiviral expression of V5-TbID-tagged mouse Emd

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Emerin (Emd Mouse)

Use

Lentiviral

Tags

V5-TbID

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-His-myc-Rbx1

Plasmid

#29506

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Rbx1 (Rbx1 Mouse)

Use

Tags

His and myc

Expression

Insect

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
TetO-FUW-Bmi1

Plasmid

#61532

Purpose

Expresses Bmi1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Bmi1 (Bmi1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
mHDAC7-Unspliced (23-938) pEF6-V5 (MJS_017)

Plasmid

#73266

Purpose

Expression of mouse HDAC7-U (23-938) in mammalian cells (V5 tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Use

Tags

His/V5

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro

Plasmid

#61441

Purpose

Dox-inducible MyoD with mRNA tag for smFISH

Depositor

Charles Gersbach

Article

Gibson et al Integr Biol (Camb). 2015 Jun 8;7(6):663-

Insert

MyoD (Myod1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Tmem98-FLAG

Plasmid

#124501

Purpose

Expresses FLAG-tagged mouse TMEM98 in mammalian cells (C-terminal tag)

Depositor

Renee van Amerongen

Article

van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

Insert

Tmem98 (Tmem98 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MyD88-CpLxIS

Plasmid

#131348

Purpose

Expresses MyD88 with 2 copies of pLxIS motif from STING fused to its C-terminus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

MyD88-CpLxIS (Myd88 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KA1396_pGL4-UBCmin-luc2/Klf4-E'

Plasmid

#124187

Purpose

Luciferase reporter driven by the Klf4 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Klf4 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNotch2#2/Cre

Plasmid

#193226

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch2#2 (Notch2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TrpV1 scFv [N221/17]

Plasmid

#182082

Purpose

Mammalian Expression of TrpV1 scFv. Derived from hybridoma N221/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting TrpV1 (Rattus norvegicus) (Trpv1 Mouse)

Use

Tags

Sortase, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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Calbindin scFv [L109/57]

Plasmid

#182071

Purpose

Mammalian Expression of Calbindin scFv. Derived from hybridoma L109/57.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Calbindin (Homo sapiens) (CALB1 Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_7C11-1 light chain

Plasmid

#198252

Purpose

recombinant expression of light chain of monoclonal anti-AMP antibody 7C11-1

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

light chain of monoclonal anti-AMP antibody 7C11-1 (mouse kappa)

Use

Tags

IL10 signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_7C11-1 heavy chain

Plasmid

#198251

Purpose

recombinant expression of heavy chain of monoclonal anti-AMP antibody 7C11-1

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

heavy chain of monoclonal anti-AMP antibody 7C11-1 (mouse IgG2a)

Use

Tags

IL10 signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_FLAG-NFL(K211TAG)

Plasmid

#182660

Purpose

Expresses mouse neurofilament light chain with a TAG codon at the position K211 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

mouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)

Use

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Mutation

K211TAG

Promoter

CMV

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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PB-Pitx2

Plasmid

#60668

Purpose

PiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Pitx2 (Pitx2 Mouse)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c2x-MT2

Plasmid

#32102

Depositor

Chris Mercogliano, Cristina Risco Ortiz, David DeRosier

Article

Mercogliano et al J Struct Biol. 2007 Oct;160(1):70-82. Ep

Insert

MT (2x) (Mt1 Mouse)

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

P-lac

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only
pMXs-ms-Klf1

Plasmid

#50785

Purpose

retroviral expression of mouse Klf1

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Klf1 (Klf1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pyc128m

Plasmid

#158778

Purpose

soluble GCaMP expression

Depositor

Zachary Knight, Jennifer Garrison

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFIN-IRBP1783-CHER

Plasmid

#44357

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

IRBP1783-CHER

Use

Lentiviral

Tags

Expression

Mutation

Promoter

murine interphotoreceptor retinoid-binding protei…

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-mNox3

Plasmid

#58337

Purpose

expresses mouse Nox3 in mammalian cells

Depositor

Botond Banfi, Karl-Heinz Krause

Article

Banfi et al J Biol Chem. 2004 Oct 29;279(44):46065-7

Insert

NADPH oxidase 3 (Nox3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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