159,428 results

1. pMD2-VSVGmut

   Plasmid

   #182229

   Purpose

   Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping

   Depositor

   Michael Birnbaum

   Article

   Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

   Insert

   VSVGmut
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47Q, R354A

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB2988

   Plasmid

   #63638

   Purpose

   pCfB2988 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW-Gata2

   Plasmid

   #193087

   Purpose

   constitutive expression of mouse Gata2 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

   Insert

   Gata2 (Gata2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PEP115E

   Plasmid

   #97401

   Purpose

   binary construct of Golgi-targeted mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Use

   Tags

   Golgi target

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLNHA-C1-Hs4E-BP1

   Plasmid

   #79438

   Purpose

   Expresses lambdaN-HA-tagged human 4E-BP1 in Mammalian cells

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   Hs4E-BP1 (EIF4EBP1 Human)
   

   Use

   Tags

   lambdaN-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PEP116E

   Plasmid

   #97402

   Purpose

   binary construct of plasma membrane-targeted mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Use

   Tags

   Plasma membrane target

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. EFR WT

   Plasmid

   #102413

   Purpose

   fluorescent tagging. Plant expression of EFR WT

   Depositor

   Silke Robatzek

   Insert

   EFR (EFR Mustard Weed)
   

   Use

   Tags

   6xHis and mgfp5

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF1a_NEUROG3_P2A_Hygro_Barcode

   Plasmid

   #120468

   Purpose

   Barcoded lentiviral vector to express NEUROG3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   NEUROG3 (NEUROG3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXRM15

   Plasmid

   #63150

   Purpose

   Met15 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

   Depositor

   Marc Gartenberg

   Article

   Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. p35S::AtSOBIR1-mCherry

    Plasmid

    #86166

    Purpose

    SOBIR1-mCherry expression

    Depositor

    Silke Robatzek

    Article

    Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

    Insert

    SOBIR1 (SOBIR1 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pαH-S-GSAS/D614G

    Plasmid

    #164566

    Purpose

    SARS-CoV-2 Spike protein with D614G mutation (S-GSAS-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-GSAS-D614G variant)
    

    Use

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQCXIZ GFP C1

    Plasmid

    #202424

    Purpose

    Encodes GFP alone

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQCXIZ GFP PODXL delta EC

    Plasmid

    #202423

    Purpose

    Expression of GFP-tagged PODXL without extracellular domain

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    deleted extracellular domain

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQCXIZ GFP PODXL 5N>Q

    Plasmid

    #202419

    Purpose

    Expression of GFP-tagged PODXL with mutated N-linked glycosylation sites

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    N33/43/104/144/360Q

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCfB2796

    Plasmid

    #63641

    Purpose

    pCfB2796 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mTagRFP-T-Paxillin-22

    Plasmid

    #58024

    Purpose

    Localization: Focal Adhesions, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Paxillin
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pRK5-HA-Ubiquitin-K48R

    Plasmid

    #17604

    Purpose

    Mammalian expression of HA tagged ubiquitin K48R

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K48R (expected to disrupt G76-K48 ubiquitin chain…

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLC6A19

    Plasmid

    #132231

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC6A19 (SLC6A19 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pDONR223-ULK4

    Plasmid

    #23849

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ULK4 (ULK4 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pLO99

    Plasmid

    #90219

    Purpose

    Complements mutations in Aspergillus nidulans gene lysB (AN5206) (mutants require lysine)

    Depositor

    Berl Oakley

    Article

    Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

    Insert

    ATET_03691
    

    Use

    Aspergillus

    Tags

    Expression

    Mutation

    Promoter

    pLac

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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