We narrowed to 44,096 results for: cha
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Plasmid#85465PurposeNatural anion conducting Channelrhodopsin with red shifted absorption. Closing kinetics 80ms. Codon optimized for mammalian expression (human/mouse)DepositorInsertSynthetic construct PsChR1 gene
UseTagsmCherryExpressionMammalianMutationPromoterCMV (+enhancer)Available SinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6-LRRC26-V5-His
Plasmid#127817PurposeExpression of human LRRC26 in mammalian cellsDepositorAvailable SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDUP1
Plasmid#223516Purposeinactive cogfp under control of Ptet, cogfp under control of PtacDepositorArticleInsertsCoGFP
CoGFP inactivated
UseTagsHis tagExpressionBacterialMutationanti-dimerization mutations S129G, 22 C154S, D156…PromoterPtac, Ptac and Ptet, and PtetAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En01
Plasmid#71534PurposeUsed to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable SinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt11
Plasmid#184512PurposeMammalian expression of Synaptotagmin 11 with pH-sensitive fluorescent proteinDepositorInsertSynaptotagmin 11 (Syt11 Mouse)
UseTagsSuper Ecliptic pHluorinExpressionMammalianMutationPromoterAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pALD4 T-
Plasmid#161481PurposeNon targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Non targeting control NTC)
UseTagsExpressionYeastMutationPromoterpALD4, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB405
Plasmid#68670PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
CLPIT Cry2PHR-mCh-RhoA
Plasmid#42959DepositorInsertCry2PHR-mCherry-RhoA (RHOA Mustard Weed, Human)
UseRetroviralTagsExpressionMammalianMutationPromoterTet-Off promoterAvailable SinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41
Plasmid#172757PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-41
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB402
Plasmid#68667PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-26
Plasmid#172751PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-26
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)
Plasmid#47387Purposeallow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectinDepositorInsertglutamate-gated chloride Channels
UseTagsExpressionMammalianMutationLeu9 prime mutated to F (Phenylalanine in M3-M4 l…PromoterCMVAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-yibDp-Erv1p
Plasmid#202483PurposeExpresses sulfydryl oxidase (Erv1p) after phosphate depletionDepositorInsertsulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoteryibDpAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Drp1 V2 S600A
Plasmid#46344DepositorAvailable SinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Drp1 V2 S600D
Plasmid#46345DepositorAvailable SinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-mCD59-T2A-GFP
Plasmid#205451Purposelentiviral plasmid for expression of mouse CD59DepositorAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKV015
Plasmid#64766PurposeRecombination-Induced Tag Exchange (RITE) Cassette, HA-GFP to a T7-RFP tag, invariable V5 tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassetteTagsExpressionMutationPromoternoneAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB131
Plasmid#68585PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsEmerald Luc fused with PEST sequence [ELuc(PEST)]ExpressionPlantMutationPromoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB435
Plasmid#68700PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-21
Plasmid#172749PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-21
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-37
Plasmid#172756PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-37
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
polyPRM5
Plasmid#175232PurposeBacterial expression and purification, mixed with polySH3 to form condensatesDepositorAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-11
Plasmid#172762PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-11
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-mRuby2
Plasmid#99117PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mRuby2DepositorInsertDIO-mRuby2
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable SinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5myc Rac1 wt
Plasmid#37030DepositorAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-ZipACR-EYFP
Plasmid#88844PurposeFast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spikingDepositorInsertZipACR-NotI-EYFP
UseLentiviralTagsEYFPExpressionMutationPromoterAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB331
Plasmid#68659PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsEmerald Luc fused with PEST sequence [ELuc(PEST)]ExpressionPlantMutationPromoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJL1-MBP-4xDQNAT
Plasmid#128390PurposeIn vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sitesDepositorInsertMaltose binding protein
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationA302T- please see depositor commentPromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Drp1 V2 S579D
Plasmid#46343DepositorAvailable SinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-1
Plasmid#172767PurposeBacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-1
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Drp1 V2 S579A
Plasmid#46342DepositorAvailable SinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB316
Plasmid#68644PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged tdTomato (tdTo…ExpressionPlantMutationPromoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-29
Plasmid#172753PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-29
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-GW182delta1
Plasmid#11592DepositorInsertGW182 (TNRC6A Human)
UseTagsGFPExpressionMammalianMutationcontains only aa1-498; SEE DEPOSITOR NOTES BELOWPromoterAvailable SinceJune 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro
Plasmid#31480DepositorInsertTraffic Light Reporter 1.1 (ani target)
UseLentiviralTagsExpressionMutationmCherry has M9S, M16L to reduce background mCherr…PromoterAvailable SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMTH PKC zeta
Plasmid#8414DepositorAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn PP-HaloTag-SYT7alpha
Plasmid#179714PurposeLentiviral expression of mouse Syt7 with N terminal HaloTagDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn PM-SYT7alpha deltaTMD
Plasmid#179720PurposeLentiviral expression of plasma membrane-targeted mouse Syt7DepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-'S-mito
Plasmid#158765PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseTagsExpressionMammalianMutationFlexible linkers between the protease and msGFP a…PromoterAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGCNK58
Plasmid#202461PurposePlasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cellsDepositorInsertResidues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable SinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 CASTOR2-HA Cterm
Plasmid#84489Purposeoverexpression of CASTOR2 with HA tagDepositorAvailable SinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB_KanR neo
Plasmid#167877PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
prp8-G736E
Plasmid#108450Purposeprp8-G736E in pRS313 backbone for expression in yeastDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationchanged G736 to EPromoterAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
ShadowG-SUMO1
Plasmid#175239PurposeBacterial expression and purification, conjugatable SUMO for SUMOylationDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA KanR neo
Plasmid#167909PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable SinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-17
Plasmid#172747PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-17
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6-LRRC55-V5-Flag-His
Plasmid#127819PurposeExpression of human LRRC55 in mammalian cellsDepositorAvailable SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-ATG13
Plasmid#171413PurposeFor the purifcation of human ULK1 complexDepositorAvailable SinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS3-lim-4P_gtACR2_SL2_eGFP(65C)_unc-54 3'UTR
Plasmid#195853PurposeExpresses inhibitory opsin gtACR2 and GFP in turning associated neuronsDepositorInsertGuillardia theta anion-conducting channelrhodopsin-2 (GtACR2)
UseTagsExpressionWormMutationPromoterlim-4Available SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsXRN1-del1174-1649_AH
Plasmid#148904PurposeMammalian Expression of HsXRN1-del1174-1649DepositorAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only