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Showing: 5581 - 5610 of 167150 results

  3. pJAT31

    Plasmid
    #204291
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-DsRed
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204291-plasmid-map-sequence-id-405841

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  5. pCALNL-DsRed

    Plasmid
    #13769
    Purpose
    Cre recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.
    Depositor
    Connie Cepko
    Article
    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10
    Insert
    DsRed2
    Use
    Cre/Lox; Flp/frt
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 20, 2007
    Availability
    Academic Institutions and Nonprofits only
    13769-plasmid-map-sequence-id-489004

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  6. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid
    #185919
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185919-plasmid-map-sequence-id-367356

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  8. pJAT30

    Plasmid
    #204294
    Purpose
    For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    MHC-DsRed
    Use
    CRISPR
    Promoter
    MHC
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204294-plasmid-map-sequence-id-405490

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  21. mCherry-ER-3

    Plasmid
    #55041
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55041-plasmid-map-sequence-id-173833

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  24. LTBP1-bio-His

    Plasmid
    #53421
    Purpose
    Expresses full-length Latent-transforming growth factor beta-binding protein 1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    LTBP1 (LTBP1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53421-plasmid-map-sequence-id-83210

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  25. pCMV-2E2-HA-mCh

    Plasmid
    #129596
    Purpose
    The encoded protein is the anti-HA frankenbody variant fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody variant-mCherry (2E2-HA scFv-mCherry)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 27, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129596-plasmid-map-sequence-id-255026

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  27. pSLIK-Hygro

    Plasmid
    #25737
    Purpose
    3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.
    Depositor
    Iain Fraser
    Article
    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available Since
    Aug. 13, 2010
    Availability
    Academic Institutions and Nonprofits only
    25737-plasmid-map-sequence-id-202030

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Showing: 5581 - 5610 of 167150 results