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Ad-Flag-XBP1s

Plasmid

#63678

Purpose

Adenoviral vector for XBP1s expression

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

Insert

XBP1 (XBP1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

spliced

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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EF1a-TET1-dCas9

Plasmid

#235593

Purpose

Tet1CD fused to N-terminus of dCas9

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

TET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)

Use

CRISPR

Tags

3xNLS, TET1 CD, and tagBFP

Expression

Mammalian

Promoter

EF1a

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pFZ1035

Plasmid

#246938

Purpose

GmWUS along with RUBY for soybean genome editing

Depositor

Feng Zhang

Article

Alok et al Plant Physiol. 2025 Dec 10:kiaf640. doi:

Insert

GmWUS, Cas9, RUBY

Use

CRISPR

Expression

Plant

Promoter

NOS, AtUbi10

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pETSXM2-pLacI-mtrCAB

Plasmid

#174618

Purpose

Bacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.

Depositor

I-Son Ng

Article

I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

Insert

mtrCAB

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLacI

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 puro - CRBN (#1)

Plasmid

#166240

Purpose

sgRNA (#1) to generate a knockout of human CRBN by targeting the middle region of Exon1

Depositor

Günter Schneider

Article

Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

Insert

CRBN (CRBN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pUHD.NFATc1nuc

Plasmid

#40616

Depositor

Jerry Crabtree

Article

Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

Insert

NFATc1nuc (NFATC1 Human)

Tags

HA

Expression

Mammalian

Mutation

S172A S175A S176A S178A S179A S181A S184A S187A S…

Promoter

CMV2

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pBoBi-hLAMP2-C-GC6s

Plasmid

#154151

Purpose

To detect the calcium ion releases close to the lysosomal surface

Depositor

Sheng-cai Lin

Article

Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

Insert

LAMP2 and GCamp6s (LAMP2 Human)

Use

Lentiviral

Mutation

WT

Promoter

CMV

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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Raf-1 GST RBD 1-149

Plasmid

#13338

Depositor

Channing Der

Article

Brtva et al J Biol Chem. 1995 Apr 28. 270(17):9809-1

Insert

Raf-1 (RAF1 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

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pCWXPGR-pTF-Kir2.1

Plasmid

#114284

Purpose

TET-inducible expression of the Kir2.1 protein

Depositor

Patrick Salmon

Insert

Kir2.1 (KCNJ2 Human)

Use

Lentiviral

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMBP-AsCas12a

Plasmid

#113430

Purpose

Expresses 10xHis-MBP fused to AsCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AsCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-f:PGC1

Plasmid

#1026

Depositor

Bruce Spiegelman

Article

Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

Insert

PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pmCer-DHX9

Plasmid

#211408

Purpose

mammalian expression of human DHX9 N-terminally fused to mCerulean

Depositor

Helmut Pospiech

Insert

DHX9 (DHX9 Human)

Tags

mCerulean

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP10

Plasmid

#22543

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP10 (USP10 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GINKO1

Plasmid

#113112

Purpose

Mammalian expression of genetically encoded green fluorescent potassium ion indicator GINKO1

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

GINKO1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pHS2

Plasmid

#81037

Purpose

HO endonuclease expression

Depositor

John McCusker

Insert

HYGMX

Expression

Yeast

Promoter

TEF

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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SIRT1.1

Plasmid

#13735

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

SIRT1 (SIRT1 Human)

Tags

His

Expression

Bacterial

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_HA1m

Plasmid

#190474

Purpose

Express the medium-affinity histamine sensor GRAB_HA1m in neurons

Depositor

Yulong Li

Article

Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

Insert

Histamine sensor GRAB_HA1m (medium affinity)

Use

AAV

Promoter

hSyn

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBb(RSF1010)1k-GFPuv

Plasmid

#106395

Purpose

IPTG-inducible trc promoter expressing gfpuv on RSF1010 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

trc

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLeAPS-GFP-blast

Plasmid

#182230

Purpose

Lentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgene

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

GFP-P2A-Blasticidin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAJM.773

Plasmid

#108527

Purpose

VanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

VanRAM + PVanCC-YFP

Expression

Bacterial

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCas3cRh

Plasmid

#133773

Purpose

Expresses all necessary components of Type I-C CRISPR-Cas system

Depositor

Joseph Bondy-Denomy

Article

Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

Inserts

RhaR (rhaR )
RhaS (rhaS )
pRhaBAD
I-C crRNA
Cas3
Cas5
Cas8
Cas7

Expression

Bacterial

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCD5-H3TX12-CO-GCN4-TEV-sfGFP-TS

Plasmid

#158739

Purpose

Expresses influenza A virus trimeric hemagglutinin A/H3/TX/12 fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Hemagglutinin A/TX/50/12 H3

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

G186V S219F

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM1-Tmem192-mRFP-3xHA

Plasmid

#134631

Purpose

bait for affinity-isolation of lysosomes

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

3xHA, TEV, and mRFP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP SNAP23

Plasmid

#101914

Purpose

Expresses SNAP23 in mammalian cells

Depositor

Thierry Galli

Article

Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

Insert

SNAP 23 (SNAP23 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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HaloTag-STAG1 Vector

Plasmid

#238689

Purpose

Express HaloTag-STAG1 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

STAG1 (STAG1 Human)

Tags

HaloTag (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDual_dsCas9_Puro

Plasmid

#214680

Purpose

Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two empty sgRNA cloning sites

Depositor

Kevin Janes

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA

Plasmid

#171619

Purpose

AAV floxed Soma-targeted Cal-Light with KA2

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_BiCHATe27_dTomato_nlsdTomato

Plasmid

#213829

Purpose

AAV construct expressing dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-mCherry-(F30-2xPepper)10

Plasmid

#129401

Purpose

mCherry mRNA reporter tagged with (F30-2xPepper)10

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

mCherry-(F30-2xPepper)10

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1 3xHA-KillerRed-OMP25

Plasmid

#174544

Purpose

localizes KillerRed to mitochondria

Depositor

Minna Roh-Johnson

Article

Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

Insert

3xHA-KillerRed-OMP25

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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