159,557 results

1. CAG-BFP-sesRNA-tTA2-WPRE-pA

   Plasmid

   #192070

   Purpose

   Expression of BFP, sesRNA in mammalian cells, with tTA2 as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for tdTom
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CL20_mEGFP_CBFB_MYH11

   Plasmid

   #205787

   Purpose

   Express mEGFP-tagged fusion protein, CBFB_MYH11 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   CBFB_MYH11 (CBFB, MYH11 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-mDyn3 pcDNA3

   Plasmid

   #36265

   Depositor

   Pietro De Camilli

   Article

   Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

   Insert

   Dynamin 3 (Dnm3 Mouse)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2012

   Availability

   Academic Institutions and Nonprofits only

4. HBV 1.3-mer X-null replicon

   Plasmid

   #65461

   Purpose

   containing 1.3 units of the X-null HBV genome (subtype ayw)

   Depositor

   Wang-Shick Ryu

   Article

   Hussain et al J Gen Virol. 2009 Sep;90(Pt 9):2272-81.

   Insert

   1.3 units of the X-null HBV genome
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-GFP-NMHC II-A

   Plasmid

   #11347

   Depositor

   Robert Adelstein

   Article

   Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

   Insert

   NMHC II-A (MYH9 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. myc-KIF5B

   Plasmid

   #127617

   Purpose

   N-terminal myc-tagged mouse KIF5B

   Depositor

   Josef Kittler

   Article

   Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

   Insert

   KIF5B (Kif5b Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-myc-GLUT4-mCherry

   Plasmid

   #64049

   Purpose

   3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation

   Depositor

   Weiping Han

   Article

   Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

   Insert

   GLUT4 (Slc2a4 Mouse)
   

   Use

   Lentiviral

   Tags

   c-Myc and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   July 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-7 asyn E46K

   Plasmid

   #105728

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

   Insert

   alpha-synuclein E46K (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E46K

   Promoter

   T7

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p5154

   Plasmid

   #175390

   Purpose

   High Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)

   Depositor

   Michael Wiles

   Article

   Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

   Type

   Empty backbone

   Use

   Mouse Targeting; Cloning vector, to be used to de…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHSN6I01

    Plasmid

    #50587

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-KRAB
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Expression

    Mutation

    dCas9 that is defective in DNA cleavage; the maiz…

    Promoter

    2×35Sp and AtU6-26p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLSB-HYG

    Plasmid

    #166699

    Purpose

    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.

    Depositor

    Robin Allshire

    Article

    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

    Inserts

    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    adh15 promoter and tRNA promoter

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTALdNC-AAVS1_T1

    Plasmid

    #80495

    Purpose

    AAVS1 TALEN expression vector (Left)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 TALEN (Left)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTALdNC-AAVS1_T2

    Plasmid

    #80496

    Purpose

    AAVS1 TALEN expression vector (Right)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 TALEN (Right)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pINDUCER21-ERG

    Plasmid

    #51301

    Purpose

    Expression of ERG after doxycycline administration

    Depositor

    George Daley

    Article

    Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

    Insert

    v-ets avian erythroblastosis virus E26 oncogene homolog (ERG Human)
    

    Use

    Lentiviral

    Tags

    n/a

    Expression

    Mutation

    full length ORF

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ144

    Plasmid

    #69296

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCJ136

    Plasmid

    #162665

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET51b-His-TEV-HaloTag7

    Plasmid

    #167266

    Purpose

    Overexpression of HaloTag7 in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HaloTag7
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. multi-shRNA vector

    Plasmid

    #12391

    Depositor

    Zuoshang Xu

    Article

    Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

    Insert

    mSOD2 shRNA (Sod2 Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACS131

    Plasmid

    #204596

    Purpose

    Histone H3.1K27M-V5

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    histone H3.1 K27M (H3C1 Human)
    

    Use

    Lentiviral

    Tags

    human histone H3.1-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST-mCherry-ARHGEF2

    Plasmid

    #207959

    Purpose

    Expression vector for ARHGEF2 (GEF-H1) with an N-terminal mCherry tag

    Depositor

    Johanna Ivaska

    Article

    Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

    Insert

    ARHGEF2 (ARHGEF2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. lentiCRISPR - EGFP sgRNA 1

    Plasmid

    #51760

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCerulean

    Plasmid

    #24756

    Depositor

    Martin Fraunholz

    Article

    Paprotka et al J Microbiol Methods. 2010 Aug 10. ():.

    Insert

    Cerulean
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pMSCV-FlagMLL-pl-ENL(5613)

    Plasmid

    #20873

    Depositor

    Robert Slany

    Article

    Bach et al Oncogene. 2009 Feb 12. 28(6):815-23.

    Insert

    MLL (KMT2A, MLLT1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCMV-hARΔ142–337

    Plasmid

    #89106

    Purpose

    Mammalian expression of human androgen receptor with deletion of amino acids 142-337 N-terminal transactivation function 1

    Depositor

    Elizabeth Wilson

    Article

    Titus et al PLoS One. 2012;7(1):e30192. doi: 10.1371

    Insert

    hAR delta 142-337 (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAR delta 142-337

    Promoter

    CMV

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

25. AAV hSYN DIO mGluR1-myc-FLAG

    Plasmid

    #166227

    Purpose

    AAV expressing cre-dependent mGluR1-myc-FLAG

    Depositor

    Jason Aoto

    Article

    Cai et al Neuron. 2021 Apr 7;109(7):1137-1149.e5.

    Insert

    metabotropic glutamate receptor 1 (Grm1 Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. LentiCRISPRv2R

    Plasmid

    #167932

    Purpose

    lentiCRISPRv2 variant with mScarlet-I fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET21a-GluRS-His

    Plasmid

    #124109

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glutamyl-tRNA ligase (gltX Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PB_iETV2_P2A_GFP_Puro

    Plasmid

    #168805

    Purpose

    PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    ETV2 (ETV2 Synthetic, Human)
    

    Use

    Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHCMV-VSVgSS-15F11-TM

    Plasmid

    #207317

    Purpose

    Functional incorporation of 15F11 scFv (anti-HA) into envelope of virions

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    15F11 ScFv
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-CAG-mBlueberry2-P2A-EGFP

    Plasmid

    #184937

    Purpose

    Expresses blue fluorescent protein mBlueberry2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    mBlueberry2-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge