We narrowed to 5,897 results for: pcDNA 3.1

1. FRT2-H2B-EGFP-V5-2A (IG157)

   Plasmid

   #99623

   Purpose

   To clone gene of interest downstream of FRT2-H2B-EGFP-V5-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   H2B-EGFP-V5
   

   Use

   Tags

   T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGAL4-DBD-HOXA13wt-IDR

   Plasmid

   #145240

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and HOXA13wt IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXA13wt-IDR (HOXA13 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTE3320

   Plasmid

   #107529

   Purpose

   Expresses human codon optimized LbCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hLbCpf1(RVR)
   

   Use

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   G532R, K538V, Y542R

   Promoter

   CMV

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Opto-GPR88

   Plasmid

   #106039

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR88; Opto-GPR88; D5)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR88 (GPR88 Human, Bovine)
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Opto-GPR119

   Plasmid

   #106040

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR119; Opto-GPR119; D6)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR119 (GPR119 Human, Bovine)
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTBL649 CHYRON2 integration construct

   Plasmid

   #126446

   Purpose

   To integrate the CHYRON2 locus at AAVS1 in human cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6-CHYRON2 hgRNA
   pCMV-puro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   CMV and human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Opto-GPR135

   Plasmid

   #106043

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR135; Opto-GPR135; D9)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR135 (GPR135 Human, Bovine)
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FRT3-Mb2-HA-mTfp1-2A (IG155)

   Plasmid

   #99621

   Purpose

   To clone gene of interest downstream of FRT3-Mb2-HA-mTfp1-2A

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2-HA-mTfp1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FRT1-Mb2YFP-2A (IG153)

   Plasmid

   #99619

   Purpose

   To clone gene of interest downstream of FRT1-Mb2YFP-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2EYFP
   

   Use

   Tags

   T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FRT2-Mb2Tomato-2A (IG154)

    Plasmid

    #99620

    Purpose

    To clone gene of interest downstream of FRT2-Mb2Tomato-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2Tomato
    

    Use

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FRT3-HA-H2B-Cerulean-2A (IG158))

    Plasmid

    #99624

    Purpose

    To clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    HA-H2B-Cerulean
    

    Use

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. VPS13C^Halo

    Plasmid

    #232864

    Purpose

    Expresses human VPS13C^Halo in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

    Insert

    VPS13C (VPS13C Human)
    

    Use

    Tags

    internal Halo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mCGN-Myc-His

    Plasmid

    #236510

    Purpose

    For expression of Cingulin (mouse) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

    Insert

    CGN (Cgn Mouse)
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pStreptagII 3C SMO

    Plasmid

    #234995

    Purpose

    Expression of a Strep-tag II labeled on its N-terminus receptor, with the Smoothened (Smo) receptor

    Depositor

    Monica Gonzalez-Magaldi, Daniel Leahy

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    SMO (SMO Human)
    

    Use

    Tags

    Streptag-II, HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hCGNL1-Myc-HA

    Plasmid

    #236515

    Purpose

    For expression of hParacingulinL1 in mammalian cells

    Depositor

    Sandra Citi

    Article

    Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

    Insert

    CGNL1 (CGNL1 Human)
    

    Use

    Tags

    Myc-HA

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP-mCGNL1-Myc

    Plasmid

    #236514

    Purpose

    For expression of Paracingulin (mouse) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

    Insert

    CGNL1 (Cgnl1 Mouse)
    

    Use

    Tags

    GFP and Myc

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-cCGNL1-HA

    Plasmid

    #236517

    Purpose

    For expression of Paracingulin (dog) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

    Insert

    CGNL1 (CGNL1 Canis lupus familiaris (dog))
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-mCGN-Myc

    Plasmid

    #236509

    Purpose

    For expression of Cingulin (mouse) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

    Insert

    CGN (Cgn Mouse)
    

    Use

    Tags

    GFP and Myc

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-mCGNL1

    Plasmid

    #236519

    Purpose

    For expression of Paracingulin (mouse) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

    Insert

    CGNL1 (Cgnl1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-cCGN-Myc

    Plasmid

    #236506

    Purpose

    For expression of CGN (dog) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

    Insert

    CGN (CGN Canis lupus familiaris (dog))
    

    Use

    Tags

    GFP and Myc

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFP-mCGN-mCGNL1-Rod1-Myc

    Plasmid

    #236526

    Purpose

    For expression of chimeric CGN containing the Rod1 domain from CGNL1 (mouse) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Flinois et al J Cell Sci. 2024 Mar 1;137(5):jcs260745.

    Insert

    CGN-CGNL1 (Cgn Mouse)
    

    Use

    Tags

    GFP and Myc

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. hA3A*-eIscBn-D

    Plasmid

    #227147

    Purpose

    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant and HMG-D domain was inserted between hA3A* and eIscBn driven by CMV promoter

    Depositor

    Dali Li

    Article

    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

    Insert

    bpNLS-hA3A*-linker-HMG-D-linker-nOgeuIscB-v3-nucleoplasmin NLS-2XUGI-bpNLS
    

    Use

    Tags

    Expression

    Mutation

    D96R/E84R/V159R/H339A

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

    Plasmid

    #191572

    Purpose

    Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.

    Depositor

    Joshua Leonard

    Article

    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

    Insert

    SARS-CoV-2 Spike Protein (S Synthetic)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    D614G and F486S (confers reduced binding affinity…

    Promoter

    CMV

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pC1-lifeact-oROS-HT

    Plasmid

    #216420

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    lifeact-oROS-HT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CCM2_d410-444_eGFP

    Plasmid

    #208877

    Purpose

    Express eGFP-CCM2 lacking amino acids 410-444 in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM2 (CCM2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    amino acids deletion : 410-444

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. KCC2 scFv [N1/12]

    Plasmid

    #190520

    Purpose

    Mammalian Expression of KCC2 scFV. Derived from hybridoma N1/12.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    KCC2 (Rattus norvegicus) recombinant scFV (Slc12a5 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGlomyc-Grhl3

    Plasmid

    #172869

    Purpose

    Mammalian expression vector with myc-tagged mouse Grhl3 coding sequence

    Depositor

    Renee van Amerongen

    Article

    Mourao et al bioRxiv 2021.06.22.449461

    Insert

    Grhl3 (Grhl3 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. nSaCas9 P742 miniABEMax V82G

    Plasmid

    #135385

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (P742 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 P742 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. nSaCas9 Q731 miniABEMax V82G

    Plasmid

    #135386

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (Q731 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 Q731 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. nSaCas9 Q737 miniABEMax V82G

    Plasmid

    #135387

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (Q737 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 Q737 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge