We narrowed to 4,854 results for: U6...

1. pSicoR-Ef1a-mCh-GFPi

   Plasmid

   #31849

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   GFP RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVscCBPTuDmiR122 Gpluc

   Plasmid

   #35654

   Depositor

   Phillip Zamore

   Article

   Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

   Insert

   TuD miR-122
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   May 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSicoR-Ef1a-mCh-Puro-Luci

   Plasmid

   #31850

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   Luciferase RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry-puromycin

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLL5.0 hCortKD-Flcortmut3YF EGFP

   Plasmid

   #187263

   Purpose

   Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

   Depositor

   Alpha Yap

   Article

   Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

   Insert

   Cortactin (CTTN Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   3YF (Y412, Y470, Y486)

   Promoter

   U6, 5'LTR

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2T CAG dCas9-10XGcn4-P2A-mCherry BlastR

   Plasmid

   #186745

   Purpose

   dCas9-10XGcn4-P2A-mCherry expression construct

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   Cas9-10XGcn4-P2A-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgRNA BRCA1 Q1779Stop

   Plasmid

   #139331

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA1 Q1779Stop using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA1 Q1779Stop using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pORANGE GSG1l-GFP KI

   Plasmid

   #131492

   Purpose

   Endogenous tagging of GSG1-l: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Gsg1l Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgCRY2

   Plasmid

   #197457

   Purpose

   Expresses sgRNA(CRY2 gene) in mammalian cells

   Depositor

   Han Kyoung Choe

   Article

   Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

   Insert

   hU6-sgCRY2-1-hU6-sgCRY2-2 (Cry2 Mouse)
   

   Use

   AAV

   Tags

   mCherry (K14E, N210D, E249G)

   Expression

   Mutation

   Promoter

   U6, hSyn

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgRNA BRCA1 T922I

   Plasmid

   #139328

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA1 T922I using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA1 T922I using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgRNA BRCA2 V2102I

    Plasmid

    #139322

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA2 V2102I using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA2 V2102I using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA BRCA2 E2772K

    Plasmid

    #139323

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA2 E2772K using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA2 E2772K using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORANGE CACNG8-GFP KI #1

    Plasmid

    #131473

    Purpose

    Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: V376)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cacng8 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330 L-FKBP-Cas9

    Plasmid

    #162728

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVsc.U6-sgFah.CMV/CB-EGFP

    Plasmid

    #121508

    Purpose

    Expresses sgRNA targeting mouse Fah intron 7 (sgFah).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgFah
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid

    #121813

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_ACTB CRISPIE

    Plasmid

    #172853

    Purpose

    Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Inserts

    Actb intron 1 sgRNA
    DRS-2 sgRNA
    CRISPIE designer exon (phase 0-0) encoding mEGFP
    mRuby3
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PLL5.0-Anillin ShRNA-Cherry-AnillinA740D, E758K

    Plasmid

    #187273

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Anillin A740D, E758K

    Promoter

    U6, CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-sgCCR5

    Plasmid

    #83930

    Purpose

    Lentiviral vector expressing an sgRNA targeting CCR5 NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgCCR5
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJZC77

    Plasmid

    #62338

    Purpose

    sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA
    COM-KRAB
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMKO.1-shEsco2

    Plasmid

    #160957

    Purpose

    Esco2 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Esco2 shRNA (Esco2 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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